Composition analysis and structural identification of polyphenols from Pinus sylvestris var. mongolica barks

ZHAO Yu-hong; ZHAI Ya-nan; WANG Zhen-yu

doi:10.13332/j.1000--1522.20140285

Journal of Beijing Forestry University > 2016 > 38(1): 125-130. > DOI: 10.13332/j.1000--1522.20140285

ZHAO Yu-hong, ZHAI Ya-nan, WANG Zhen-yu. Composition analysis and structural identification of polyphenols from Pinus sylvestris var. mongolica barks[J]. Journal of Beijing Forestry University, 2016, 38(1): 125-130. DOI: 10.13332/j.1000--1522.20140285

Citation:

PDF (1674 KB)

Composition analysis and structural identification of polyphenols from Pinus sylvestris var. mongolica barks

1.
1 College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040 , P. R. China;
2.
2 School of Food Science and Engineering, Harbin Institute of Technology,Harbin, Heilongjiang, 150090, P. R. China.

More Information

Received Date: November 14, 2014
Revised Date: January 04, 2015
Published Date: January 30, 2016

Graphical Abstract

Abstract

Abstract

The composition of polyphenols from Pinus sylvestris L. var. mongolica were analyzed and their structures were identified with UV-VIS, IR, HPLC and LC-MS. The UV-VIS results showed that there are absorption peaks in regions of 240-- 280 nm and 300-- 400 nm, indicating that flavonoids exist in the pine polyphenols. Fourier Transform Infrared Spectrum analysis showed that a strong and broad absorption peak at 3 400 cm-1 is generated from stretching and vibration of phenolic hydroxyl Ar-OH and absorption peaks at 1 390-- 1 315 cm-1 due to characteristics of in-plane bending vibration of hydroxyl δO—H, which indicated that hydroxyl groups existed in the pine polyphenols. Combined application of HPLC and LC-MS showed that there are seven components in P. sylvestris L. var. mongolica polyphenols, i.e., catechuic acid, rutin, chlorogenic acid, gallic acid, sinapic acid-dextrose glucoside, peony-3- glucoside or centaurea-3-(6-o-acetyl)-glucose glycoside, and sinapic acid peonidin-3-sophoroside composition.
- Pinus sylvestris var. mongolica,
- polyphenols,
- composition analysis,
- structural identification

FullText(HTML)

References (19)

References

[1]	LIAZID A, SCHWARZ M, VARELA R M, et al. Evaluation of various extraction techniques for obtaining bioactive extracts from pine seeds[J]. Food and Bioproducts Processing, 2010, 88(2): 247- 252.
[1]	ZHAO H T, WANG Z Y, CHENG C L, et al.Advances in antioxidant activity mechanism research and structure-activity relationship of pine polyphenols[J]. Science and Technology of Food Industry, 2012, 33(2): 458- 461.
[2]	PINELO M, RUBILAR M, SINEIRO J, et al. Extraction of antioxidant phenolics from almond hulls (Prunus amygdalus) and pine sawdust (Pinus pinaster)[J]. Food Chemistry, 2004, 85(2): 267- 273.
[2]	GU J, ZHANG Y.Effective components of the pine bark[J]. Heilongjiang Medical Science, 2009, 32(3): 4- 9.
[3]	ZHAO Y H, ZHAI Y N, WANG Z Y. Extraction of polyphenlols from Pinus sylvestris var mongolica and comparison of extraction method[J]. Science and Technology of Food Industry, 2013, 34(4): 304- 309.
[3]	赵海田,王振宇,程翠林,等. 松多酚类活性物质抗氧化构效关系与作用机制研究进展[J]. 食品工业科技, 2012, 33(2): 458- 461.
[4]	BRAGA M E M, SANROS R, SEABRA I J, et al. Fractioned SFE of antioxidants from maritime pine bark[J]. The Journal of Supercritical Fluids, 2008, 47(1): 37- 48.
[4]	ZHAO Y H, ZHAI Y N, DANG Y, et al. Optimization of purification conditions of polyphenols from Pinus sylvestris var. mongolica using response surface methodology[J].Journal of Beijing Forestry University, 2014, 36(1): 138- 142.
[5]	ANN S T, JAN O, ANETA W. Antioxidant activity of the phenolic compounds of hawthorn, pine and skullcap[J]. Food Chemistry, 2007,103:853- 859.
[5]	LIU R, HE J,WANG Z Y. Purification of polyphenols from Pinus sylvestris L.var. mongolica barks by macroporous resin[J]. Science and Technology of Food Industry, 2013, 34(11): 201- 209.
[6]	SEKIDO Y. Genomic abnormalities and signal transduction dysregulation in malignant mesothelioma cells[J]. Cancer Science, 2010, 101(1): 1- 6.
[7]	NIGAM N, BHUI K, PRASAD S, et al. [6]-gingerol induces reactive oxygen species regulated mitochondrial cell death pathway in human epidermoid carcinoma A431 cells[J]. Chemico-biological Interactions, 2009, 181(1): 77- 84.
[8]	BONELLO P, BLODGETT J T. Pinus nigra-Sphaeropsis sapinea as a model pathosystem to investigate local and systemic effects of fungal infection of pines[J]. Physiological and Molecular Plant Pathology, 2003, 63(5): 249- 261.
[9]	FREITAS A M, ALMEIDA M T R. ALMEIDA C R. Antiviral activity-guided fractionation from Araucaria angustifolia leaves extract[J]. Journal of Ethno Pharmacology, 2009, 126(3): 512- 517.
[10]	TURTOLA S, SALLAS L, HOLOPAINEN J K, et al. Long-term exposure to enhanced UV-B radiation has no significant effects on growth or secondary compounds of outdoor-grown Scots pine and Norway spruce seedlings[J]. Environmental Pollution, 2006, 144(1): 166- 171.
[11]	顾剑, 张宇. 樟子松树皮有效成分的初步研究[J]. 黑龙江医药科学, 2009, 32(3): 4- 9.
[12]	赵玉红, 翟亚楠, 王振宇. 樟子松树皮中松多酚的提取工艺研究及提取方法比较[J]. 食品工业科技, 2013, 34(4): 304- 309.
[13]	赵玉红, 翟亚楠, 党媛,等. 响应面法优化樟子松树皮松多酚纯化工艺研究[J]. 北京林业大学学报, 2014, 36(1): 138- 142.
[14]	刘荣, 何娇, 王振宇. 大孔树脂对樟子松树皮多酚的纯化工艺研究[J]. 食品工业科技, 2013, 34(11): 201- 209.

Cited By

Cited by

Periodical cited type(9)

1.	范越，王鹏，王静怡，郭庆启. 基于大数据统计分析松多酚的研究现状和热点展望. 食品工业科技. 2023(24): 34-42 .
2.	刘梦楠，李晓庆，周军，姚桢，彭茜，李斌，刘卫. 酚醛树脂基铝电解用炭素材料的静态浸润过程. 有色金属(冶炼部分). 2022(04): 49-56 .
3.	马妮，刘慧燕，方海田，胡海明，辛世华，杨小萍，刘洪涛. 红枣多酚提取工艺优化、成分及抗氧化活性分析. 食品工业科技. 2022(16): 246-254 .
4.	任维维，吴烨婷，梁宗瑶，李珉梦，魏园园，段旭昌. 青柿子提取物的抗氧化、抑菌、抑癌活性研究. 中国食品学报. 2021(11): 29-38 .
5.	贡小辉，朱益灵，魏渊，孔令东，韩邦兴，欧阳臻. 石斛甲醇提取物HPLC-MS分析及4种多酚测定. 中成药. 2020(05): 1223-1228 .
6.	蔡铭，陈思，骆少磊，杨开，孙培龙. 膜分离与醇沉技术纯化猴头菇粗多糖的比较. 食品科学. 2019(09): 83-90 .
7.	绰尔鹏，赵玉红. 热风干燥温度对老山芹品质的影响. 现代食品科技. 2019(07): 127-136 .
8.	王晓梅，张忠山，吴酬飞，张立钦. 马尾松松针多酚的提取及其抗氧化活性. 湖州师范学院学报. 2018(04): 30-34 .
9.	王菲儿，杨跃军，徐剑，巫岳，蔡剑峰，傅青，郑向炜，刘源才，金郁. 板栗壳棕色素的抗氧化活性及其活性成分的分离、鉴定. 食品工业科技. 2018(24): 86-91+96 .

Other cited types(20)

Get Citation

PDF

XML

Article views (2192) PDF downloads (37) Cited by(29)

Turn off MathJax

Article Contents

Abstract

References

Composition analysis and structural identification of polyphenols from Pinus sylvestris var. mongolica barks

Abstract

References

Cited by

Periodical cited type(9)

Other cited types(20)

Catalog

Export File

Citation

Format

Content