Gene expression profile of aspen hybrid cuttings in two developing stages of the adventitious root.

YAN Shao-peng; SHANG Yan-ru; LENG Shu-jiao; YANG Rui-hua; WANG Qiu-yu

doi:10.13332/j.1000-1522.20130230

Journal of Beijing Forestry University > 2015 > 37(10): 9-13. > DOI: 10.13332/j.1000-1522.20130230

YAN Shao-peng, SHANG Yan-ru, LENG Shu-jiao, YANG Rui-hua, WANG Qiu-yu. Gene expression profile of aspen hybrid cuttings in two developing stages of the adventitious root.[J]. Journal of Beijing Forestry University, 2015, 37(10): 9-13. DOI: 10.13332/j.1000-1522.20130230

Citation:

PDF (867 KB)

Gene expression profile of aspen hybrid cuttings in two developing stages of the adventitious root.

1.
1 College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang, 150040, P. R. China;
2.
2 Horticultural Branch, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, 150069, P. R. China.

More Information

Received Date: December 24, 2013
Published Date: October 30, 2015

Graphical Abstract

Abstract

Abstract

We used Populus tremula × P. tremuloides softwood cuttings as experimental material, and observed morphological and anatomical changes of the cuttings during the rooting process of adventitious roots. Solexa high-throughput sequencing was performed for the parts of cuttings where adventitious roots generated 6 days and 12 days after cutting (DAC) at transcriptional level. Anatomical observation showed that the induction stage of the adventitious root primordia was 6 days after cutting and the elongation stage of adventitious roots was 12 days after cutting. A total of 2 328 differentially expressed genes were obtained by the Solexa high-throughput sequencing. The significantly differential expression genes were classified after their functions annotation, and then the KEGG passway analysis was carried out. On this basis, the biochemical metabolic and signal transduction pathways of these differentially expressed genes were determined.
- hybrid aspen,
- gene expression profile,
- cutting,
- differential expression

FullText(HTML)

References (16)

References

[1]	闫绍鹏.欧美山杨杂种无性系低温胁迫下几种生理指标的遗传变异[D].哈尔滨:东北林业大学,2004.
[1]	YAN S P. Genetic variation among the hybrid clones of Populus tremula × P. tremloides in several physiological characteristics under low temperature stress[D].Harbin: Northeast Forestry University,2004.
[2]	ROSIER C L,FRAMPTON J, GOLDFARB B,et al. Growth stage,auxin type,and concentration,influence rooting of Virginia pine stem cuttings[J]. Hortscience,2004,39(6):1397-1402.
[2]	LENG S J. Analysis of the digital gene expression profiling of Populus tremula × P. tremloides cuttings development during adventitious roots induction and adventitious roots elongation[D]. Harbin: Northeast Forestry University,2012.
[3]	YAN S P, YANG R H, LENG S J, et al. High-fluxed DNA sequencing technology and its application in agricultural science research[J]. Chinese Agricultural Science Bulletin, 2012,28(30): 171-176.
[3]	SYROS T,YUPSANIS T,ZAFIRIADIS H,et al. Activity and isoforms of peroxidases,lignin and anatomy,during adventitious rooting in cuttings of Ebenus cretiea L[J]. Journal of Plant Physiology,2004,16(1):69-77.
[4]	冷淑娇.欧美山杨插穗不定根发育的诱导期和伸长期数字表达谱分析[D].哈尔滨:东北林业大学,2012.
[5]	BRINKER M, ZYL L, LIU W B, et al. Microarray analyses of gene expression during adventitious root development in Pinus contorta[J]. Plant Physiology, 2004, 135:1526-1539.
[6]	RIGAL A, YORDANOV Y S, PERRONE I, et al. The AINTEGUMENTA LIKE1 homeotic transcription factor PtAIL1 controls the formation of adventitious root primordia in poplar[J]. Plant Physiol, 2012, 160:1996-2006.
[7]	LI R Q,YU C,LI Y R,et al. SOAP2: an improved ultrafast tool for short read alignment[J]. Bioinformatics,2009, 25(15): 1966-1967.
[8]	MORTAZAVI A, WILLIAMS B A, MCCUE K, et al. Mapping and quantifying mammalian transcriptomes by RNA-Seq[J]. Nature Methods,2008,5(7):621-628.
[9]	THOMAS P, SCHIEFELBEIN J. Cloning and characterization of an actin depolymerizing factor gene from grape (Vitis inifera L.) expressed during rooting in stem cuttings[J]. Plant Science, 2002, 162:283-288.
[10]	SWARUP R, FRIML J, MARCHANT A, et al. Localization of the auxin permease AUX1 suggests two functionally distinct hormone transport pathways operate in the Arabidopsis root apex[J]. Genes Dev, 2001,15: 2648-2653.
[11]	WU G S, DANIEL R L, EDGAR P. Spalding, mutations in Arabidopsis multidrug resistance-like ABC transporters separate the roles of acropetal and basipetal auxin transport in lateral root development[J]. The Plant Cell, 2007,19(6): 1826-1837.
[12]	DING X H, CAO Y L, HUANG L L, et al. Activation of the indole-3-acetic acid-amido synthetase GH3-8 suppresses expansin expression and promotes salicylate-and jasmonate-independent basal immunity in rice[J]. The Plant Cell January,2008,20(1):228-240.
[13]	闫绍鹏,杨瑞华,冷淑娇,等. 高通量测序技术及其在农业科学研究中的应用[J].中国农学通报,2012,28(30):171-176.

Cited By

Cited by

Periodical cited type(0)

Other cited types(1)

Get Citation

PDF

XML

Article views (1510) PDF downloads (33) Cited by(1)

Turn off MathJax

Article Contents

Abstract

References

Gene expression profile of aspen hybrid cuttings in two developing stages of the adventitious root.

Abstract

References

Cited by

Periodical cited type(0)

Other cited types(1)

Catalog

Export File

Citation

Format

Content