Upstream regulators of ThVHAc1.

YANG Gui-yan; ZHAO Zhen; ZHAO Yu-lin; ZHANG Feng-jiao; GAO Cai-qiu

doi:10.13332/j.1000-1522.20140486

Journal of Beijing Forestry University > 2015 > 37(12): 1-6. > DOI: 10.13332/j.1000-1522.20140486

YANG Gui-yan, ZHAO Zhen, ZHAO Yu-lin, ZHANG Feng-jiao, GAO Cai-qiu. Upstream regulators of ThVHAc1.[J]. Journal of Beijing Forestry University, 2015, 37(12): 1-6. DOI: 10.13332/j.1000-1522.20140486

Citation:

YANG Gui-yan, ZHAO Zhen, ZHAO Yu-lin, ZHANG Feng-jiao, GAO Cai-qiu. Upstream regulators of ThVHAc1.[J]. Journal of Beijing Forestry University, 2015, 37(12): 1-6. DOI: 10.13332/j.1000-1522.20140486

Citation:

YANG Gui-yan, ZHAO Zhen, ZHAO Yu-lin, ZHANG Feng-jiao, GAO Cai-qiu. Upstream regulators of ThVHAc1.[J]. Journal of Beijing Forestry University, 2015, 37(12): 1-6. DOI: 10.13332/j.1000-1522.20140486

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Upstream regulators of ThVHAc1.

1.
1 State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 150040, Harbin, Heilongjiang, P. R. China;
2.
2 Laboratory of Walnut Research Center, College of Forestry, Northwest A & F University, 712100, Yangling, Shaanxi, P. R. China.

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Received Date: January 04, 2015
Published Date: December 30, 2015

Graphical Abstract

Abstract

Abstract

ThVHAc1 gene was reported to be responsive to drought, salt and heavy metal stresses, and overexpressed ThVHAc1 yeast could improve effectively multi-stress tolerance. To further understand the stress regulation mechanism of ThVHAc1, we screened the upstream regulators that could specifically recognize the cis-elements DOF and MYB in the ThVHAc1 promoter by yeast one-hybrid assay. The results showed that the ThMYB3 gene could specifically recognize the MYB motif and the promoter segment that contains MYB motif, and the ThDof2 gene could specifically recognize the DOF motif and the promoter segment that includes DOF motif in the ThVHAc1 promoter. However, ThMYB3 and ThDof2 could not recognize the mutated motifs and promoter segments containing these mutated motifs. Then ThMYB3 and ThDof2 were independently constructed as effecter vector, and MYB and DOF motifs as well as their mutates and promoters were independently constructed as reporter vectors for co-expression assays. And the results of co-transient expression of the effecters and reporters confirmed that ThDof2 and ThMYB3 can specifically bind to the ThVHAc1 promoter. ThMYB3 and reporters containing MYB motif and ThVHAc1 promoter segments including MYB motif, ThDof2 and reporters containing DOF motif and the ThVHAc1 promoter segments including DOF motif could stain the GUS expression in tobacco leaves and showed high GUS activities. However, other co-expression referred to mutated motifs could not activate the GUS expression, indicating that only normal cis-elements and promoter segments could be recognized by effecters to activate the GUS expression. Our study suggests the effectiveness of ThMYB3 and ThDof2 as ThVHAc1 upstream regulators.
- Tamarix hispida,
- yeast one hybrid,
- VHAc gene,
- promoter,
- upstream regulator

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References

[1]	DIETZ K J, TAVAKOLI N, KLUGE C, et al. Significance of the V-type Atpase for the adaptation to stressful growth conditions and its regulation on the molecular and biochemical level[J] . Journal of Experimental Botany, 2001, 52: 1969-1980.
[1]	HAO Y L, ZHU B Z, LUAN C G, et al. Cloning and identification of sunflower seed-specific promoter Ha ds10 G1 [J] . Journal of Agricultural Biotechnology, 2006, 14(6): 922-925.
[2]	KANE P, KUEHN M, HOWALD-STEVENSON I, et al. Assembly and targeting of peripheral and integral membrane subunits of the yeast vacuolar H+-ATPase[J] . Journal of Biological Chemistry, 1992, 267(1): 447-454.
[3]	UMEMOTO N, YOSHIHISA T, HIRATA R,et al. Roles of the Vma3 gene product, subunit c of the vacuolar membrane H+-ATPase on vacuolar acidification and protein transport-a study with Vma3-disrupted mutants of Saccharomyces cerevisiae[J] . Journal of Biological Chemistry, 1990, 265(30): 18447-18453.
[4]	TYAGI W, RAJAGOPAL D, SINGLA-PAREEK S L, et al. Cloning and regulation of a stress-regulated Pennisetum glaucum vacuolar ATPase c gene and characterization of its promoter that is expressed in shoot hairs and floral Organs[J] . Plant and Cell Physiology, 2005, 46(8): 1411-1422.
[5]	NITZ I, BERKEFELD H, PUZIO P S,et al. Pyk10, a seedling and root specific gene and promoter from Arabidopsis thaliana[J] . Plant Science, 2001, 161(2): 337-346.
[6]	郝彦玲, 朱本忠, 栾春光, 等. 向日葵种子特异性启动子Ha ds10 G1的克隆及其功能验证[J] . 农业生物技术学报, 2006, 14(6): 922-925.
[7]	ZAREI A, KRBES A P, YOUNESSI P,et al. Two GCC boxes and AP2/ERF-domain transcription factor ORA59 in jasmonate/ethylene-mediated activation of the PDF1.2 promoter in Arabidopsis[J] . Plant Molecular Biology, 2011, 75(4-5): 321-331.
[8]	ZHENG L, LIU G, MENG X,et al. A WRKY gene from Tamarix hispida, ThWRKY4, mediates abiotic stress responses by modulating reactive oxygen species and expression of stress-responsive genes[J] . Plant Molecular Biology, 2013, 82(4-5): 303-320.
[9]	GAO C, WANG Y, JIANG B,et al. A novel vacuolar membrane H+-ATPase c subunit gene(ThVHAc1) from Tamarix hispida confers tolerance to several abiotic stresses in Saccharomyces cerevisiae[J] . Molecular Biology Reports, 2011, 38(2): 957-963.
[10]	WANG L, XU C, WANG C,et al. Characterization of a eukaryotic translation initiation factor 5A homolog from Tamarix androssowii involved in plant abiotic stress tolerance[J] . BMC Plant Biology, 2012, 12(1): 118.
[11]	GAO C, WANG Y, LIU G,et al. Expression profiling of salinity-alkali stress responses by large-scale expressed sequence tag analysis in Tamarix hispida[J] . Plant Molecular Biology, 2008, 66(3): 245-258.
[12]	YANAGISAWA S, SCHMIDT R J. Diversity andsimilarity among recognition sequences of Dof transcription factors[J] . The Plant Journal, 1999, 17(2): 209-214.
[13]	JI X, LIU G, LIU Y,et al. The bZIP protein from Tamarix hispida, ThbZIP1, Is ACGT elements binding factor that enhances abiotic stress signaling in transgenic Arabidopsis[J] . BMC Plant Biology, 2013, 13(1): 151,
[14]	HOSODA K, IMAMURA A, KATOH E,et al. Molecular structure of the GARP family of plant Myb-related DNA binding motifs of the Arabidopsis response regulators[J] . The Plant Cell Online, 2002, 14(9): 2015-2029,
[15]	WANG L, QIN L, LIU W, et al. A novel ethylene-responsive factor from Tamarix hispida, ThERF1, is a GCC-box and DRE-motif binding protein that negatively modulates abiotic sress tolerance in Arabidopsis[J] . Physiologia Plantarum, 2014, 152(1): 84-87
[16]	SUN C, PALMQVIST S, OLSSON H,et al. A novel WRKY transcription factor, Susiba2, participates in sugar signaling in barley by binding to the sugar-responsive elements of the iso1 promoter[J] . The Plant Cell Online, 2003, 15(9): 2076-2092.

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