Selection of distant hybridization parents for triploid LA hybrid lilies

CAO Qin-zheng; GAO Xue; JIA Gui-xia

doi:10.13332/j.1000-1522.20150199

Journal of Beijing Forestry University > 2016 > 38(2): 96-104. > DOI: 10.13332/j.1000-1522.20150199

CAO Qin-zheng, GAO Xue, JIA Gui-xia. Selection of distant hybridization parents for triploid LA hybrid lilies[J]. Journal of Beijing Forestry University, 2016, 38(2): 96-104. DOI: 10.13332/j.1000-1522.20150199

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Selection of distant hybridization parents for triploid LA hybrid lilies

Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding; National Engineering Research Center for Floriculture; Beijing Laboratory of Urban and Rural Ecological Environment; College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, P. R. China.

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Received Date: May 28, 2015
Revised Date: October 09, 2015
Published Date: February 28, 2016

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Abstract

Abstract

In order to investigate the applicability of LA hybrids as the breeding parents and select parents with a better fertility in LA×Asiatic lilies, 62 crossings were conducted with 26 LA cultivars and 10 Asiatic cultivars used as parents. Genomic in situ hybridization (GISH) technique was applied for hybrid identification and analysis of chromosomal composition in some hybrid progenies. Main results were obtained as follows: 1) LA triploid hybrids are usually male-sterile, but they can be crossed with diploid or tetraploid Asiatic hybrids as a female parent. 2) Compared with diploid Asiatic hybrids (AA), tetraploid Asiatic hybrids (AAAA) have a better fertility when crossed with LA hybrids. 3) Seven cross combinations with a better fertility were selected: ‘Nashville’×‘Nello’,‘Freya’×‘ValdiSole’,‘Desiderio’×‘Detroit’,‘Samur’×‘Nello’,‘Batistero’×‘Regata’,‘Roussea-u’×‘Val di Sole’ and ‘Ercolano’×‘Benlica’. The results of 62 crossings revealed that 3 LA hybrids as female parents (‘Freya’,‘Nashville’ and ‘Orange Tycoon’) and 6 Asiatic hybrids as male parents (‘Val di Sole’,‘Nello’,‘Regata’,‘Detroit’,‘Benlica’ and ‘Black out’) had high seed-setting rates. 4) The chromosomes recombination between aneuploid eggs that allotriploid hybrids produced and the genomes has high potential in introgression breeding. This study further develops the breeding potential of triploid LA hybrids, and lays the foundation for breeding of commercial lily varieties.
- triploid LA hybrid lily,
- Asiatic hybrids,
- fertility of distant hybridization,
- GISH

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References

[1]	LI S L,SHI L,ZHANG J Z,et al.Recent advances in lily breeding [J].Acta Horticulturae Sinica,2006,33(1):203-210.
[1]	李守丽,石雷,张金政,等.百合育种研究进展[J].园艺学报,2006,33(1):203-210.
[2]	VAN TUYL J M,MAAS I W G M,LIM K B.Introgression in interspecific hybrids of lily[J].Acta Horticulturae,2002,570:213-218.
[2]	LI K H.Study on the potential of allotriploid lilies (Lilium) in Breeding[D].Hangzhou: Zhejiang University,2011.
[3]	XIE S L.Research on techniques of lily(Lilium) interspecific hybridization and resistant breeding to Fusarium wilt disease[D].Xian: Northwest AF University,2010.
[3]	ZHOU S J,RAMANNA M S,VISSER R G F,et al.Genome composition of triploid lily cultivars derived from sexual polyploidization of Longiflorum×Asiatic hybrids(Lilium)[J].Euphytica,2008,160:207-215.
[4]	李克虎.异源三倍体百合Lilium育种潜力研究[D].杭州:浙江大学,2011.
[4]	ZHOU S J.Discrimination of the genomes in BC1 progeny of Asiatic lily and Oriental lily using GISH[J].Acta Horticulturae Sinica,2003,30(4):485-486.
[5]	ZHOU G X, LI K H, ZHANG X X, et al. Studies on ploidy levels, pollen fertility and interploid hybridization of Asiatic lilies[J]. Acta Horticulturae Sinica,2011, 38(4): 733-739.
[5]	谢松林.百合远缘杂交与抗立枯病育种技术研究[D].西安:西北农林科技大学,2010.
[6]	VAN TUYL J M. Interspecific hybridization of flower bulbs:a review[J]. Acta Horticulturae,1997,430:465-476.
[6]	YUAN G L, XU P,GONG H X,et al. Lily introgression breeding using triploids cultivars as maternal parents[J]. Journal of Agricultural Biotechnology, 2013, 21(11): 1321-1327.
[7]	LIM K B, RAMANNA M S, DE JONG J H, et al.Indeterminate meiotic restitution (IMR): a novel type of meiotic nuclear restitution mechanism detected in interspecific lily hybrids by GISH[J]. Theoretical and Applied Genetics,2001,103:219-230.
[7]	ZHOU S J.Technical ways of breeding modern lily (Lilium) cultivars and the mechanisms of the special phenomena of their hybridizations[J].Journal of Agricultural Biotechnology,2014,22(10):1189-1194.
[8]	XU S C,GUAN J,CAO H Y,et al. Study on the relationship between lily crossbreeding affinity and ploidy levels[J].Molecular Plant Breeding,2014,12(2):316-322.
[8]	周树军.基因组荧光原位杂交区分百合回交一代的不同基因组[J].园艺学报,2003,30(4):485-486.
[9]	YU S W,ZHANG D P,SONG Y C.The uptodate advance and use of genomic in situ hybridization(GISH) in plant[J].Journal of Wuhan Botanical Research,2001,19(3):248-254.
[9]	ZHOU S J. Intergenomic recombination and introgression breeding in Longiflorum × Asiatic lilies(Lilium)[D]. Wageningen: Wageningen University, 2007.
[10]	ZHOU S,RAMANNA M S,VISSER R G F, et al.Analysis of the meiosis in the F1 Hybrids of Longiflorum×Asiatic(LA) of lilies(Lilium) using genomic in situ hybridization[J].Genet Genomles,2008,35:687-695.
[11]	ZHOU S J, YUAN G L, XU P, et al.Study on lily introgression breeding using allotriploids as maternal parents in interploid hybridizations[J]. Breeding Science, 2014,64: 97-102.
[12]	周桂雪,李克虎,张线线,等.亚洲百合品种倍性、花粉育性及其杂交研究[J].园艺学报,2011,38(4):733-739.
[13]	袁国良,徐萍, 宫红霞, 等.用三倍体作母本实现百合种质渗入育种[J]. 农业生物技术学报,2013,21(11):1321-1327.
[14]	周树军.现代百合品种培育的技术途径及其杂交特殊现象的机制[J].农业生物技术学报,2014,22(10):1189-1194.
[15]	徐顺超,管洁,曹荷艳,等.百合杂交育种亲和性与染色体倍性相关性研究[J].分子植物育种,2014,12(2):316-322.
[16]	ZHOU S J, ZHOU G X, LI K H. Euploid endosperm of triploid×diploid/tetraploide crosses results in aneuploid embryo survival in Lilium [J]. Hortscience, 2011, 46: 558-562.
[17]	ZHOU S J, LI K H, ZHOU G X. Analysis of endosperm development of allotriploid × diploid/tetraploid crosses in Lilium [J].Euphytica,2012, 184: 401-412.
[18]	余舜武,张端品,宋运淳.基因组原位杂交的新进展及其在植物中的应用[J].武汉植物学研究,2001,19(3):248-254.
[19]	LIM K B, RAMANNA M S, DE JONG J H, et al. Evaluation of BC2 progenies derives from 3x×2x and 3x×4x crosses of lilium hybrids: a GISH analysis[J]. Theoretical and Applied Genetics, 2003, 106(3): 568-574.
[20]	BARBA-GONZALEZ R, VAN SILFHOUT R A A, RAMANNA M S, et al.Progenies of allotriploids of Oriental×Asiaticlilies (Lilium) examined by GISH analysis[J]. Euphytica, 2006,151(2): 243-250.
[21]	XIE S, RAMANNA M S, VAN TUYL J M. Simultaneous identification of three different genomes in Lilium hybrids through multicolour GISH[J]. Acta Horticulturae, 2010,855: 299-303.

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