Genetic diversity of Machilus pauhoi assessed by SRAP markers.

ZHOU Peng; LIN Wei; ZHU Qin; ZHOU Xiang-bin; WU Lin-ying; CHEN Xiao-yang

doi:10.13332/j.1000-1522.20150423

Journal of Beijing Forestry University > 2016 > 38(9): 16-24. > DOI: 10.13332/j.1000-1522.20150423

ZHOU Peng, LIN Wei, ZHU Qin, ZHOU Xiang-bin, WU Lin-ying, CHEN Xiao-yang. Genetic diversity of Machilus pauhoi assessed by SRAP markers.[J]. Journal of Beijing Forestry University, 2016, 38(9): 16-24. DOI: 10.13332/j.1000-1522.20150423

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Genetic diversity of Machilus pauhoi assessed by SRAP markers.

1.
1 College of Forestry and Landscape Architecture, South China Agricultural University,Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou, Guangdong, 510642, P.R. China;
2.
2 Guangdong Vocational College of Ecological Engineering, Guangzhou, Guangdong, 510520, P.R. China;
3.
3 School of Life Science, Jiaying University, Meizhou, Guangdong, 514015, P.R. China.

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Received Date: November 20, 2015
Published Date: September 29, 2016

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Abstract

Abstract

The sequence-related amplified polymorphism(SRAP)markers were used to assess the genetic diversity and relationship of 23 Machilus pauhoi Kanehira provenances from seven provinces. Twelve primers were screened out which were used for SRAP-PCR. The results showed that: 1) a total of 157 clear bands were amplified, and 115 bands were polymorphic (the percentage of polymorphic band, PPB=73.24%); 2) the polymorphism information content (PIC) values ranged from 0.406 to 0.502, with an average of 0.476; 3) the molecular variance analysis (AMOVA) revealed that genetic variation mainly existed within provenance (72.19%) and few among provenances (27.81%), showing that the variation of M. pauhoi within provenances was the major source of genetic diversity; 4) the cluster analysis results suggest that 23 provenances should be divided into four categories: provenances from Hunan, Guangdong, Guangxi and Jiangxi are clustered in the same group; all provenances from Zhejiang Province are together; Wuyuan and Huangshan provenances are classed together; and all provenances from Fujian Province are clustered into one group, The classification results reflect geographic trends significantly. The result of this study provides theoretical basis for germplasm resources preservation and breeding programs of M. pauhoi.
- Machilus pauhoi Kanehira,
- sequence-related amplified polymorphism,
- genetic diversity

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References (45)

References

[1]	吴振伙,吴兆平,全尚龙.刨花润楠综合利用价值及其育苗技术[J].现代农业科技, 2008(20):55.
[1]	WU Z H, WU Z P, QUAN S L. Utilization value and breeding techniques of Machilus pauhoi Kanehira[J]. Modern Agricultural Sciences and Technology, 2008(20):55.
[2]	胡希华.刨花润楠的优良特性及育苗栽培技术[J].湖南林业科技,2006,33(1):65-66.
[3]	HU X H.Excellent characteristics and seedling cultivation techniques of Machilus pauhoi Kanehira[J]. Hunan Forestry Science Technology, 2006, 33(1): 65-66.
[4]	钟智群,谭梓峰,杨志玲,等.刨花楠生长发育特点及开发前景分析[J].湖南林业科技, 1997,24(2):50-51.
[5]	ZHONG Z Q, TAN Z F, YANG Z L, et al.The growth characteristics and development prospects of Machilus pauhoi Kanehira[J]. Hunan Forestry Science Technology, 1997, 24(2): 50-51.
[6]	LI G, QUIROS C F. Sequence-related amplified polymorpgism (SRAP) , a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J]. Theoretical and Applied Genetics, 2001, 103: 455-461.
[7]	王国泽,左福元,曾兵.SRAP分子标记的研究进展及在植物上的应用[J].畜牧与兽医, 2013,45(9):92-95.
[8]	WANG G Z, ZUO F Y, ZENG B.The development of SRAP molecular markers and the application in plant[J]. Animal Husbandry ＆Veterinary Medicine, 2013, 45(9): 92-95.
[9]	LI G, GAO M, YANG B, et al. Gene for gene alignment between the Brassica and Arabidopdsis genomes by direct transcriptome mapping[J]. Theoretical Applied Genetics, 2003, 107(1): 168-180.
[10]	董爱香,王涛,徐进, 等.用SRAP分子标记分析一串红品种资源的亲缘关系[J].北京林业大学学报,2012,34(5):134-138.
[11]	DONG A X, WANG T, XU J, et al. Genetic relationship of germplasm resources of Salvia splendens using SRAP marker[J]. Journal of Beijing Forestry University, 2012, 34(5):134-138.
[12]	李达,熊兴耀,于晓英, 等.红花檵木SRAP反应体系的建立及优化[J].中南林业科技大学学报,2008,28 (5) :22-27.
[13]	LI D, XIONG X Y, YU X Y, et al. Establishment and optimization of SRAP reaction system in Loropetalum chinense var. rubrum[J]. Journal of Central South University of Forestry Technology, 2008, 28(5):22-27.
[14]	姜树坤,马慧,刘君, 等.利用SRAP标记分析玉米遗传多样性[J].分子植物育种,2007,5(3):412-416.
[15]	JIANG S K, MA H, LIU J, et al.Genetic diversity in maize inbred lines revealed by SRAPs marker[J]. Molecular Plant Breeding, 2007, 5(3): 412-416.
[16]	王燕,龚义勤,赵统敏,等.番茄SRAP-PCR体系优化与品种分子鉴定[J].南京农业大学学报,2007,30(1):23-29.
[17]	WANG Y, GONG Y Q, ZHAO T M, et al.Optimization of SRAP-PCR system and cultivar molecular identification in tomato (Lycopersicon esculentum L.) [J]. Journal of Nanjing Agricultural University, 2007, 30(1): 23-29.
[18]	李明芳,卢诚,刘兴地,等.小桐子25个无性系遗传多样性的SRAP分析[J].中国农学通报,2014(1):26-31.
[19]	LI M F, LU C, LIU X D, et al.Genetic diversity analysis among 25 Clones of Jatropha curcas by SRAP markers[J]. Chinese Agricultural Science Bulletin, 2014(1): 26-31.
[20]	姜艳,刘进平.胡椒SRAP反应体系的建立和优化[J].中国农学通报,2012,28(31):141-145.
[21]	JIANG Y, LIU J P.Establishment and optimization of black pepper SRAP reaction system[J]. Chinese Agricultural Science Bulletin, 2012, 28(31):141-145.
[22]	YUKO K, CHUNLAN L, SHUNTARO W, et al. Development of microsatellites in Machilus thunbergii (Lauraceae), a warm-temperate coastal tree species in Japan[J]. American Journal of Botany,2012, 99(7):265-267.
[23]	TSUNEKI S, MORI K, KANEKO S, et al. Identification and characterization of eight microsatellite loci in Machilus pseudokobu (Lauraceae), an endemic species of the Bonin Islands[J].Conservation Genetics, 2009, 10(6):2009-2011.
[24]	ZHANG R, ZHOU Z C, JIN G Q, et al. Genetic diversity and differentiation within three species of the family Lauraceae in southeast China[J]. Biochemical Systematics and Ecology, 2012, 44:317-324.
[25]	刘玉香,宋晓琛,江香梅.润楠ISSR-PCR优化反应体系建立及引物筛选[J].林业科技开发, 2013,27(5):24-28.
[26]	LIU Y X, SONG X C, JIANG X M.Optimization of ISSR-PCR reaction system and primers screening in Machilus pingii[J]. China Forestry Science and Technology, 2013, 27(5):24-28.
[27]	姜荣波.红楠群体遗传多样性及优良种源选择[D].北京:中国林业科学研究院, 2011.
[28]	JIANG R B. Population genetic diversity and selection of superior provenances of Machilus thunbergii[D]. Beijing: Chinese Academy of Forestry, 2011.
[29]	YEH F C, YANG R C, BOYLE T. Microsoft window-based freeware for population genetic analysis, quick user guide: popgene version 1.31[EB/OL]. Edmonton: University of Alberta, 1999.
[30]	ROHLF F J. NTSYS-pc numerical taxonomy and multivariate analysis system: verson 2.1[EB/OL]. New York: Exeter Software, 2000.
[31]	PEAKALL R, SMOUSE P E. GenAlEx 6.5: genetic analysis in excel. population genetic software for teaching and research: an update[J]. Bioinformatics, 2012,28(19):2537-2539.
[32]	VAIMAN D, MERCIER D, MOAZAMI-GOUDARZI K, et al. A set of 99 cattle microsatellites, characterization, synteny mapping and polymorphism[J]. Mammalian Genome, 1994,5(5):288-297.
[33]	XIE W G, ZHANG X Q, CAI H W, et al. Genetic diversity analysis and transferability of cereal EST-SSR markers to orchardgrass (Dactylis glomerata L.)[J]. Biochemical Systematics and Ecology, 2010,38(4):740-749.
[34]	KUBITZKI K, ROHWER J G, BITTRICH V. The families and genera of vascular plants. Vol.2: flowering plants[M]. Berlin: Springer-Verlag, 1993.
[35]	WRIGHT S. The genetical structure of populations[J]. Nature, 1950,166: 247-249.
[36]	王峥峰,高山红,田胜尼,等.南亚热带森林片断化对厚壳桂种群遗传结构的影响[J].生物多样性, 2005, 13(4):324-331.
[37]	WANG Z F, GAO S H, TIAN S N, et al. Genetic structure of Cryptocarya chinensis in fragmented lower subtropical forests in China based on ISSR markers[J]. Biodiversity Science, 2005, 13(4): 324-331.
[38]	王中生,安树青,冷欣,等.岛屿植物舟山新木姜子居群遗传多样性的RAPD分析[J].生态学报, 2004, 24(3):414-422.
[39]	WANG Z S, AN S Q, LENG X, et al. Population genetic diversity of the insular plant Neolitsea sericea based on random amplified polymorphic DNA (RAPD) [J]. Acta Ecologica Sinica, 2004, 24(3):414-422.
[40]	陈俊秋,慈秀芹,李巧明,等.樟科濒危植物思茅木姜子遗传多样性的ISSR分析[J].生物多样性, 2006, 14(5):410-420.
[41]	CHEN J Q, CI X Q, LI Q M, et al. Genetic diversity of Litsea szemaois, an endangered species endemic to China, detected by inter-simple sequence repeat (ISSR) [J]. Biodiversity Science, 2006, 14(5):410-420.
[42]	慈秀芹.樟科濒危植物思茅木姜子(Litsea szemaois)的保护遗传学研究[D].西双版纳:中国科学院研究生院(西双版纳热带植物园), 2007.
[43]	CI X Q.Studies on conservation genetics of endangered species Litsea szemaois (Lauraceae) [D]. Xishuangbanna: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 2007.
[44]	WRIGHT S. Isolation by distance[J]. Genetics, 1943, 28(2): 114-138.

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