Extraction and purification conditions of anthocyanin from the fruit of Aronia melanocarpa.

Anthocyanins were extracted from Aronia melanocarpa with water, 50%-100% ethanol and 50%-100% methanol. The results showed that the optimal yield of anthocyanins was with 80% ethanol. Four single factor experiments were designed for extracting time, solid-liquid ratio, pH and temperature. Based on the single factor experiment analysis, the mathematical model was established by Box-Behnken response surface design. The results showed that the optimum extracting conditions were: extracting time 110 min, pH 2, extracting temperature 34 ℃, solid-liquid ratio 1∶16.5. Under these conditions, anthocyanins yield reached 6.648 mg/g. AB-8 was chosen as the optimal resin after the static adsorption and desorption testing. The optimum purification conditions were 2 mg/mL of solution concentration, 1∶25 of diameter to length, 2.2 BV, pH 2 and 80% ethanol with the elution flow rate of 2.0 BV/h, the anthocyanins purity increased 11.5 times.