Over-expression of RPEase gene promotes the growth and development of Arabidopsis thaliana

WANG Dan; AN Yi; HAN Xiao; ZHOU Yang-yan; WANG Hou-ling; GUO Hui-hong; XIA Xin-li; YIN Wei-lun

doi:10.13332/j.1000-1522.20150507

Journal of Beijing Forestry University > 2016 > 38(5): 67-76. > DOI: 10.13332/j.1000-1522.20150507

WANG Dan, AN Yi, HAN Xiao, ZHOU Yang-yan, WANG Hou-ling, GUO Hui-hong, XIA Xin-li, YIN Wei-lun. Over-expression of RPEase gene promotes the growth and development of Arabidopsis thaliana[J]. Journal of Beijing Forestry University, 2016, 38(5): 67-76. DOI: 10.13332/j.1000-1522.20150507

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Over-expression of RPEase gene promotes the growth and development of Arabidopsis thaliana

National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, P. R. China.

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Received Date: December 27, 2015
Revised Date: December 27, 2015
Published Date: May 30, 2016

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Abstract

Abstract

The Calvin cycle is an irreplaceable pathway for photosynthetic organisms to assimilate CO2 from the air and therefore plays a crucial role in plant growth and development. The ribulose-phosphate 3-epimerase (RPEase: EC 5.1.3.1) is one of the integral enzymes in the Calvin cycle regeneration phase. In our study PdRPE gene was cloned from fast-growing hybrid poplar NE-19 (Populus nigra × (Populus deltoids × Populus nigra)), and then PdRPE was transformed into Arabidopsis thaliana by the floral dip method. Using PCR and Green Fluorescent Protein (GFP) detection methods, we obtained the CaMV35S:PdRPE:GFP transgenic plants. Then we validated the growth status and physiological indexes of wild type (Col-0), overexpressing (OxPdRPE), mutant (rpe) and complementary (rpe/PdRPE) lines. The results indicated that in overexpressing lines RPEase activity increased significantly (P<0.05). When well-watered, the overexpressing lines had lower leaf stomatal density, larger stomatal size, improved water use efficiency and higher net photosynthetic rate, showing a better growth condition and more starch accumulation. When exposed to 10 days of short-term drought, the net photosynthetic rate and water use efficiency of overexpressing lines were still significantly higher than those of the other three strains (P<0.01). Therefore, we reach the conclusion that over-expression of RPEase gene can promote the plant growth and development and enhance the resistance of short-duration drought in Arabidopsis thaliana.
- poplar,
- Calvin cycle,
- RPEase,
- growth,
- development,
- water use efficiency

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Over-expression of RPEase gene promotes the growth and development of Arabidopsis thaliana

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