Intrasectional and intersectional cross breeding of <i>Paeonia</i> and karyotype analysis and SSR identification of some hybrids

LIU Jian-xin; YANG Liu-hui; WEI Dong-xia; YU Xiao-nan

doi:10.13332/j.1000-1522.20160181

Journal of Beijing Forestry University > 2017 > 39(4): 72-78. > DOI: 10.13332/j.1000-1522.20160181

LIU Jian-xin, YANG Liu-hui, WEI Dong-xia, YU Xiao-nan. Intrasectional and intersectional cross breeding of Paeonia and karyotype analysis and SSR identification of some hybrids[J]. Journal of Beijing Forestry University, 2017, 39(4): 72-78. DOI: 10.13332/j.1000-1522.20160181

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Intrasectional and intersectional cross breeding of Paeonia and karyotype analysis and SSR identification of some hybrids

College of Landscape Architecture, National Engineer Research Center for Floriculture, Beijing Key Laboratory for Flower Germplasm Innovation and Molecular Breeding, Beijing Forestry University, Beijing, 100083, P.R.China

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Received Date: May 26, 2016
Revised Date: November 10, 2016
Published Date: March 31, 2017

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Abstract

Abstract

In order to select the proper cross combinations with high compatibility, hybridization was carried out between Paeonia lactiflora 'Zhu Sha Pan' as female parent and 6 cultivars of Sect. Paeonia and 4 cultivars of Sect. Moutan as male parents. Meanwhile, hybrids were identified by karyotype analysis and SSR markers. The results showed that:1) Hybridization in Sect. Paeonia had a high compatibility, with an average seed setting rate of 5 seeds per flower; interspecific hybridizations of Sect. Paeonia had a poor compatibility; sectional hybridizations were incompatible, only cross combination 'Zhu Sha Pan' ×'Dao Da Chen' seeded. 2) Karyotype analysis showed that hybrids ZC4, ZC5, ZC6 and ZC7 were triploid; Among 50 SSR primers, 7 polymorphic markers were amplified and the hybrids ZC4, ZC5, ZC6 and ZC7 contained specific bents of parents, which were true hybrids. The results suggested that interspecific hybridization between 'Zhu Sha Pan' and 'Garden Peace', 'Pink Teacup', 'Cream Delight' could seed; sectional hybridization 'Zhu Sha Pan' ×'Dao Da Chen' could break the cross incompatibility. SSR markers could be applied for the early hybrid identification of Paeonia.
- herbaceous peony,
- distant hybridization,
- karyotype,
- SSR,
- hybrid identification

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References (25)

References

[1]	HONG D Y, PAN K Y, ZHOU S L, et al. Peonies of the world[M]. Kew: Royal Botanic Gardens, 2010.
[2]	李嘉珏.中国牡丹与芍药[M].北京:中国林业出版社, 1995. LI J J. Peonies of China[M]. Beijing: China Forestry Publishing House, 1995.
[3]	邓衍明, 叶晓青, 佘建明, 等.植物远缘杂交育种研究进展[J].华北农学报, 2011, 26(增刊): 52-55. http://d.old.wanfangdata.com.cn/Periodical/hbnxb2011z1013 DENG Y M, YE X Q, SHE J M, et al. The research progress on distant hybridization of plant breeding[J]. Acta Agriculture Boreali-Sinica, 2011, 26(Suppl.): 52-55. http://d.old.wanfangdata.com.cn/Periodical/hbnxb2011z1013
[4]	陆光沛, 于晓南.美国芍药牡丹协会金牌奖探析[J].中南林业科技大学学报, 2009, 29(5): 191-194. doi: 10.3969/j.issn.1673-923X.2009.05.020 LU G P, YU X N. An analysis of the American Peony Society's gold medal[J]. Journal of Central South University of Forestry and Technology, 2009, 29 (5): 191-194. doi: 10.3969/j.issn.1673-923X.2009.05.020
[5]	SMITH D. ('Martha Washington'×'Golden Era') intersectional hybrids[J]. Paeonia, 2000, 30 (2): 6.
[6]	何桂梅, 孙菊芳, 成仿云.牡丹芍药组间杂交种的起源及其发展[J].北方园艺, 2006 (6): 106-108. doi: 10.3969/j.issn.1001-0009.2006.06.056 HE G M, SUN J F, CHENG F Y. The origin and development of intersectional cross of tree peony and herbaceous[J]. Northern Horticulture, 2006 (6): 106-108. doi: 10.3969/j.issn.1001-0009.2006.06.056
[7]	吴静, 成仿云, 张栋. '正午'牡丹的杂交利用及部分杂种AFLP鉴定[J].西北植物学报, 2013, 33 (8): 1551-1557. http://d.old.wanfangdata.com.cn/Periodical/xbzwxb201308008 WU J, CHENG F Y, ZHANG D. Utilizing 'High Noon' in the crossing breeding of tree peonies and early identification of some hybrids by AFLP markers[J]. Acta Botanica Boreali-Occidentalia Sinica, 2013, 33 (8): 1551-1557. http://d.old.wanfangdata.com.cn/Periodical/xbzwxb201308008
[8]	丁晓六, 刘佳, 赵红霞, 等.现代月季和玫瑰杂交后代的鉴定与评价[J].北京林业大学学报, 2014, 36 (5): 123-130. doi: 10.13332/j.cnki.jbfu.2014.05.005 DING X L, LIU J, ZHAO H X, et al. Hybrid identification and morphological evaluation of modern roses (Rosa hybrida)×Rosa rugosa [J]. Journal of Beijing Forestry University, 2014, 36 (5): 123-130. doi: 10.13332/j.cnki.jbfu.2014.05.005
[9]	刘伟.不同倍性燕麦种质核型鉴定和SSR指纹图谱构建[D].北京: 中国农业科学院, 2012. http://cdmd.cnki.com.cn/Article/CDMD-82101-1012415683.htm LIU W. Karyotype characterization and SSR fingerprint of different of different ploidy oat germplasm[D]. Beijing: Chinese Academy of Agricultural Sciences, 2012. http://cdmd.cnki.com.cn/Article/CDMD-82101-1012415683.htm
[10]	徐顺超, 管洁, 曹荷艳, 等.百合杂交育种亲和性与染色体倍性相关性研究[J].分子植物育种, 2014, 12 (2): 316-322. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201402015 XU S C, GUAN J, CAO H Y, et al. Study on the relationship between lily cross-breeding affinity and ploidy levels[J]. Molecular Plant Breeding, 2014, 12 (2): 316-322. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201402015
[11]	管洁, 焦雪辉, 吴锦娣, 等.用ISSR分子标记鉴定亚洲百合杂种F1代[J].分子植物育种, 2013, 11 (3): 415-420. http://d.old.wanfangdata.com.cn/Periodical/fzzwyz201303021 GUAN J, JIAO X H, WU J D, et al. Identification of Asian lily hybrid F1 by using ISSR[J]. Molecular Plant Breeding, 2013, 11 (3): 415-420. http://d.old.wanfangdata.com.cn/Periodical/fzzwyz201303021
[12]	YUAN J H, CHENG F Y, ZHOU S L. Hybrid origin of Paeonia×yananensis revealed by microsatellite markers, chloroplast gene sequences, and morphological characteristics[J]. International Journal of Plant Sciences, 2010, 171: 409-420. doi: 10.1086/651228
[13]	ZHANG J J, SHU Q Y, LIU Z A, et al. Two EST-derived marker systems for cultivar identification in tree peony[J]. Plant Cell Report, 2012, 31: 299-310. doi: 10.1007/s00299-011-1164-1
[14]	GUO X, LI Y, LI C, et al. Analysis of the Dendrobium officinale transcriptome reveals putative alkaloid biosynthetic genes and genetic markers[J]. Gene, 2013, 527(1): 131-138. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=e7fd90588afe74dfaa256c61bba79278
[15]	YU H P, CHENG F Y, ZHONG Y, et al. Development of simple sequence repeat (SSR) markers from Paeonia ostii to study the genetic relationships among tree peonies (Paeoniaceae)[J]. Scientia Horticulturae, 2013, 164: 58-64. doi: 10.1016/j.scienta.2013.06.043
[16]	WU J, CAI C F, CUI H L, et al. Characterisation and development of EST-SSR markers in tree peony using transcriptome sequences[J]. Molecular Breeding, 2014(34): 1853-1866. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=125369a8a183ee657b719aa49fa70283
[17]	马慧, 于晓南. 4个观赏芍药新品种染色体核型分析[J].河北农业大学学报, 2013, 36 (6): 61-65. http://d.old.wanfangdata.com.cn/Periodical/hbnydxxb201306012 MA H, YU X N. Studies on karyotypes of four cultivars in herbaceous peony[J]. Journal of Agricultural University of Hebei, 2013, 36 (6): 61-65. http://d.old.wanfangdata.com.cn/Periodical/hbnydxxb201306012
[18]	李懋学, 陈瑞阳.关于植物核型分析的标准化问题[J].武汉植物学研究, 1985, 3(4): 297-302. doi: 10.1002-polb.21779/ LI M X, CHEN R Y. A suggestion on the standardization of karyotype analysis in plants[J]. Journal of Wuhan Botanical Research, 1985, 3(4): 297-302. doi: 10.1002-polb.21779/
[19]	STEBBINS G L. Chromosomal evolution in higher plants[M]. London: Edward Aronld, 1971: 87-93.
[20]	ARANO H. Cytological studies in subfamily Carduoideae (Compositae) of Japan Ⅸ[J]. Botanical Magazine, 1963(76): 32-39.
[21]	GUO X R. Karyotype analysis of some formosan gymnosperms[J]. Taiwania, 1972, 17(1): 66-80. http://www.twscholar.com/Publication/alDetailedMesh?DocID=0372333X-197203-201303080035-201303080035-66-80
[22]	JI L J, SILVA T J, ZHANG J J, et al. Development and application of 15 novel polymorphic microsatellite markers for sect. Paeonia (Paeonia L.)[J]. Biochemical Systematics and Ecology, 2014, 54: 257-266. doi: 10.1016/j.bse.2014.02.009
[23]	张建军, 季丽静, 刘仲赫, 等.引进观赏芍药新种质的SSR指纹图谱构建[J].东北林业大学学报, 2015, 43(3): 70-74. doi: 10.3969/j.issn.1000-5382.2015.03.016 ZHANG J J, JI L J, LIU Z H, et al. Constructing SSR fingerprinting for introduced new germplasm of Paeonia L.[J]. Journal of Northeast Forestry University, 2015, 43(3): 70-74. doi: 10.3969/j.issn.1000-5382.2015.03.016
[24]	张栋.牡丹远缘杂交及部分杂交后代的AFLP分子标记鉴定[D].北京: 北京林业大学, 2008. http://cdmd.cnki.com.cn/Article/CDMD-10022-2008084683.htm ZHANG D. Distant cross-breeding in tree peony and identification of some hybrids by AFLP[D]. Beijing: Beijing Forestry University, 2008. http://cdmd.cnki.com.cn/Article/CDMD-10022-2008084683.htm
[25]	王越岚.牡丹的杂交育种及组间杂种育性的研究[D].北京: 北京林业大学, 2009. http://cdmd.cnki.com.cn/Article/CDMD-10022-2009161421.htm WANG Y L. Cross-breeding in tree peony and fertility research of intersectional hybrids[D]. Beijing: Beijing Forestry University, 2009. http://cdmd.cnki.com.cn/Article/CDMD-10022-2009161421.htm

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Intrasectional and intersectional cross breeding of Paeonia and karyotype analysis and SSR identification of some hybrids