Analysis of appearance features and genetic diversity of <i>Cytospora chrysosperma</i> causing poplar canker

ZHANG Jun-e; FAN Xin-lei; LIANG Ying-mei; TIAN Cheng-ming

doi:10.13332/j.1000-1522.20160257

Journal of Beijing Forestry University > 2017 > 39(7): 76-86. > DOI: 10.13332/j.1000-1522.20160257

ZHANG Jun-e, FAN Xin-lei, LIANG Ying-mei, TIAN Cheng-ming. Analysis of appearance features and genetic diversity of Cytospora chrysosperma causing poplar canker[J]. Journal of Beijing Forestry University, 2017, 39(7): 76-86. DOI: 10.13332/j.1000-1522.20160257

Citation:

PDF (4302 KB)

Analysis of appearance features and genetic diversity of Cytospora chrysosperma causing poplar canker

1.
Key Laboratory for Silviculture and Forest Conservation of Ministry of Education, Beijing Forestry University, Beijing, 100083, P. R. China
2.
Museum of Beijing Forestry University, Beijing, 100083, P. R. China

More Information

Received Date: August 10, 2016
Revised Date: February 05, 2017
Published Date: June 30, 2017

Graphical Abstract

Abstract

Abstract

In order to investigate the appearance features and genetic diversity of Cytospora chrysosperma, as well as their relationships with different factions of the host poplar and their geographic origin, in this paper, 106 samples of C. chrysosperma from 14 provinces of China were collected and observed. And the growth of strains was recorded when being cultivated to analyze the cultivated morphological diversity of strains. Then, 34 samples with obviously different background information were selected and genetic diversity was analyzed using two signing methods of SRAP and ISSR. The results showed that C. chrysosperma presented stable cultivated traits in the same strain under different conditions. While different strains had obvious morphological differences, but there were no obvious correlations between their cultivated traits and their host plants or geographic origins. 12 SRAP primers and 20 ISSR primers with decent polymorphism were obtained in total, which were used to analyze and calculate Nei's index of the population diversity. The results were 0.31 (SRAP) and 0.28 (ISSR), indicating that large genetic differences existed among varied strains. On the other hand, population genetic structure was analyzed from the perspective of the host. The results showed that the total population genetic diversity was 0.27 and the genetic diversity within population was 0.23, which can explain 87% of the overall diversity and the genetic separation coefficient (G_ST) was 0.13. All of above showed that the genetic variation mainly existed within the group. From the view of geographical position, the total genetic diversity was 0.28, genetic diversity within population was 0.13, which can explain 48% of overall diversity and the coefficient of genetic separation (G_ST) was 0.52. That means the diversity within population is equivalent to that among populations. The geographic migrations among populations have great impacts on the overall genetic separation. The genetic diversity levels of the populations show a decreasing trend from west to east.
- poplar canker,
- culture morphology,
- SRAP,
- ISSR,
- diversity

FullText(HTML)

References (35)

References

[1]	张星耀, 骆有庆.中国森林重大生物灾害[M].北京:中国林业出版社, 2003: 120-128. ZHANG X Y, LUO Y Q. Major forest diseases and insect pests in China[M]. Beijing: China Forestry Publishing House, 2003: 120-128.
[2]	刘栗君, 李旭东.杨树插条烂皮病的田间调查[J].防护林科技, 2013, 114(3): 79-80. doi: 10.3969/j.issn.1005-5215.2013.03.034 LIU L J, LI X D. Field investigation of poplar cuttings rotten skin disease[J]. Protective Forest Science and Technology, 2013, 114(3): 79-80. doi: 10.3969/j.issn.1005-5215.2013.03.034
[3]	苑国. 9种无公害杀菌剂对杨树烂皮病菌的室内毒力测定[J].现代园艺, 2013(2): 10-12. doi: 10.3969/j.issn.1006-4958.2013.02.008 YUAN G. Laboratory toxicity of nine pollution-free fungicides to Cytospora chrysosperma causing poplar canker[J]. Modern Gardening, 2013(2): 10-12. doi: 10.3969/j.issn.1006-4958.2013.02.008
[4]	FOTOUBIFAR K B, HEDJAROUDE G A, LEUCHTMANN A. ITS rDNA phylogeny of Iranian strains of Cytospora and associated teleomorphs[J]. Mycologia, 2010, 102(6): 1369-1382. doi: 10.3852/10-034
[5]	FAN X L, HYDE K D, YANG Q, et al. Cytospora species associated with canker disease of three anti-desertification plants in northwestern China[J]. Phytotaxa, 2015, 197(4): 227-244. doi: 10.11646/phytotaxa.197.4.1
[6]	FAN X L, LIANG Y M, MA R, et al. Morphological and phylogenetic studies of Cytospora (Valsaceae, Diaporthales) isolates from Chinese scholar tree, with description of a new species[J]. Mycoscience, 2014, 55(4): 252-259. doi: 10.1016/j.myc.2013.10.001
[7]	FAN X L, TIAN C M, YANG Q, et al. Cytospora from Salix in northern China[J]. Mycotaxon, 2014, 129: 303-315. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=a52048ffa59d217bfd4eda727499ceb7
[8]	张星耀, 陈海燕, 梁军, 等.金黄壳囊孢菌(Cytospora chrysosperma)的培养性状和营养体亲和性[J].西北农林科技大学学报(自然科学版), 2007, 35(3): 99-105. doi: 10.3321/j.issn:1671-9387.2007.03.021 ZHANG X Y, CHEN H Y, LIANG J, et al. Cultural morphology and vegetative compatibility of Cytospora chrysosperma isolates[J]. Journal of Northwest Agriculture and Forestry University (Natural Science Edition), 2007, 35(3): 99-105. doi: 10.3321/j.issn:1671-9387.2007.03.021
[9]	徐梅卿, 周旭东, 朴春根.中国不同栽培区杨树品系及其病害种类[J].林业科学研究, 2009, 22(5): 705-714. doi: 10.3321/j.issn:1001-1498.2009.05.016 XU M Q, ZHOU X D, PU C G. Populus cultivation clones in different cultivated area and its diseases in China[J]. Forest Research, 2009, 22(5): 705-714. doi: 10.3321/j.issn:1001-1498.2009.05.016
[10]	王德宏.不同杨树品种对杨树烂皮病菌的抗性差异[J].辽宁林业科技, 1988(6): 31-33. http://www.cnki.com.cn/Article/CJFDTotal-LNLK198806008.htm WANG D H. Resistance differences of varieties of Populus to Cytospora chrysosperma[J]. Liaoning Forestry Science and Technology, 1988(6): 31-33. http://www.cnki.com.cn/Article/CJFDTotal-LNLK198806008.htm
[11]	郝宏, 汪太振, 姜永范, 等.杨树烂皮病危害损失测定及防治指标的研究[J].森林病虫通讯, 1993(4): 5-7. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=slbctx199304003 HAO H, WANG T Z, JIANG Y F, et al. Study on the determination of poplar rot disease damage loss and control index[J]. Forest Pest and Disease, 1993(4): 5-7. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=slbctx199304003
[12]	姚金, 全兴军, 陶华, 等.黑龙江省杨树烂皮病的发病规律及其综合防治[J].中国西部科技, 2009, 8(7): 73-74. doi: 10.3969/j.issn.1671-6396.2009.07.037 YAO J, QUAN X J, TAO H, et al. The occurrence regularity and integrated control of poplar rot disease in Heilongjiang Province[J]. Science and Technology of West China, 2009, 8(7): 73-74. doi: 10.3969/j.issn.1671-6396.2009.07.037
[13]	仝国彦.杨树腐烂病的发生及综合防治[J].现代农业科技, 2009(3): 128. doi: 10.3969/j.issn.1007-5739.2009.03.080 TONG G Y. Occurrence and integrated control of poplar rot disease[J]. Modern Agricultural Science and Technology, 2009(3): 128. doi: 10.3969/j.issn.1007-5739.2009.03.080
[14]	浦景树.杨树烂皮病的综合防治措施[J].农村实用科技信息, 2013(7): 27. http://d.old.wanfangdata.com.cn/Periodical/ncsykjxx201307025 PU J S. Comprehensive prevention and control measures of poplar skin rot[J]. Rural Practical Technology Information, 2013(7): 27. http://d.old.wanfangdata.com.cn/Periodical/ncsykjxx201307025
[15]	崔磊.杨树品种抗烂皮病相关基因的RAPD分析[D].哈尔滨: 东北林业大学, 2005. http://cdmd.cnki.com.cn/article/cdmd-10225-2005136735.htm CUI L. RAPD analysis on resistant genes to canker of poplar cultivators[D]. Harbin: Northeast Forestry University, 2005. http://cdmd.cnki.com.cn/article/cdmd-10225-2005136735.htm
[16]	杨明秀, 宋瑞清.中国金黄壳囊孢菌的致病性分化及遗传多样性[J].林业科学, 2013, 49(6): 115-121. doi: 10.3969/j.issn.1672-8246.2013.06.019 YANG M X, SONG R Q. Pathogenic differentiation and genetic diversity of Cytospora chrysosperma in China[J]. Science Silvae Sinicae, 2013, 49(6): 115-121. doi: 10.3969/j.issn.1672-8246.2013.06.019
[17]	ABBASI K, ABBASI S, FOTOUBIFAR K B, et al. Genetic diversity of Cytospora chrysosperma isolates obtained from Iranian walnut trees using molecular markers[J]. African Journal of Biotechnology, 2011, 10(70): 15710-15716. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=Open J-Gate000003936253
[18]	臧睿.陕西苹果树腐烂病菌不同分离株生物学特性及致病性研究[D].杨凌: 西北农林科技大学, 2006. http://cdmd.cnki.com.cn/Article/CDMD-10712-2006179875.htm ZANG R. Studies on biological characteristics and pathogenicity of different isolates of Cytospora isolated from apple tree[D]. Yangling: Northwest A & F University, 2006. http://cdmd.cnki.com.cn/Article/CDMD-10712-2006179875.htm
[19]	王艳丽.杨树烂皮病病原菌Cytospora spp.的系统发育和遗传多样性研究[D].南京: 南京林业大学, 2013. http://cdmd.cnki.com.cn/Article/CDMD-10298-1013045857.htm WANG Y L. The phylogeny and genetic diversity of Cytospora spp. species isolated from poplar canker[D]. Nanjing: Nanjing Forestry University, 2013. http://cdmd.cnki.com.cn/Article/CDMD-10298-1013045857.htm
[20]	张星耀, 赵仕光, 吕全, 等.树木溃疡病病原真菌类群分子遗传多样性研究Ⅱ-Botryosphaeria属28S rDNA-PCR-RFLP和RAPD解析[J].林业科学, 2000, 36(2): 75-81. http://d.old.wanfangdata.com.cn/Periodical/lykx200002012 ZHANG X Y, ZHAO S G, LV Q, et al. Molecular genetic diversity of pathoeneticg fungal group causing tree canker Ⅱ-28S rDNA-PCR-RFLP and RAPD analysis of Botryosphaeria spp.[J]. Science Silvae Sinicae, 2007, 36(2): 75-81. http://d.old.wanfangdata.com.cn/Periodical/lykx200002012
[21]	田呈明, 康振生, 李振岐, 等.落叶松-杨栅锈菌遗传分化的RAPD分析[J].林业科学, 2000, 36(5): 54-58. doi: 10.3321/j.issn:1001-7488.2000.05.009 TIAN C M, KANG Z S, LI Z Q, et al. Analysis of the genetic specialization of Melampsora larici-populina with RAPD markers[J]. Science Silvae Sinicae, 2000, 36(5): 54-58. doi: 10.3321/j.issn:1001-7488.2000.05.009
[22]	曹支敏, 杜林, 王秦虎, 等.中国落叶松-杨栅锈菌遗传多样性研究[J].菌物学报2012, 31(4): 510-522. http://d.old.wanfangdata.com.cn/Periodical/jwxt201204008 CAO Z M, DU L, WANG Q H, et al. Genetic diversity of poplar rust fungus Melampsora larici-populina in China[J]. Mycosystema, 2012, 31(4): 510-522. http://d.old.wanfangdata.com.cn/Periodical/jwxt201204008
[23]	YAN B Q, LI Z Z, HUANG H W, et al. Genetic diversity and population differentiation of chestnut blight fungus, Cryphonectria parasitica, in China as revealed by RAPD[J]. Biochemical Genetics, 2007, 45(5/6): 487-506. doi: 10.1007/s10528-007-9090-1
[24]	TANG L H, XIAO Y, LI L, et al. Analysis of genetic diversity among Chinese Auricularia auricula cultivars using combined ISSR and SRAP markers[J]. Current Microbiology, 2010, 61(2): 132-140. doi: 10.1007-s00284-010-9587-4/
[25]	SHAO Y C, XU L, CHEN F S. Genetic diversity analysis of Monascus strains using SRAP and ISSR markers[J]. Mycoscience, 2011, 52(4): 224-233. doi: 10.1007/S10267-010-0087-Y
[26]	LI G, QUIROS C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a PCR reaction: its application to mapping and gene tagging in Brassica[J]. Theoretical and Applied Genetics, 2001, 103(2): 455-461. doi: 10.1007/s001220100570
[27]	李蕊倩, 何瑞, 张跃兵, 等.镰刀菌ISSR标记体系的建立及遗传多样性分析[J].中国农业科学, 2009, 42(9): 3139-3146. http://d.old.wanfangdata.com.cn/Periodical/zgnykx200909015 LI R Q, HE R, ZHANG Y B, et al. Establishment ISSR reaction systerm of Fusarium and its analysis of genetic diversity[J]. Scientia Agricultura Sinica, 2009, 42(9): 3139-3146. http://d.old.wanfangdata.com.cn/Periodical/zgnykx200909015
[28]	徐焰平.茶树内生芒果球座菌(Guignardia mangiferae)的鉴定生态分布和遗传多样性分析[D].福州: 福建师范大学, 2009. http://cdmd.cnki.com.cn/Article/CDMD-10394-2010035227.htm XU Y P. The identification, ecological distribution, and genetic diversity analysis of Guignardia mangiferae from tea plant[D]. Fuzhou: Fujian Normal University, 2009. http://cdmd.cnki.com.cn/Article/CDMD-10394-2010035227.htm
[29]	BIHON W, BURGESS T, SLIPPERS B, et al. High levels of genetic diversity and cryptic recombination is widespread in introduced Diplodia pinea populations[J]. Australasian Plant Pathol, 2012, 41(1): 41-46. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=e40a7d712cd6b13d5042bd7fac703c38
[30]	GELL I, LARENA I, MELGAREJO P. Genetic diversity in Monilinia laxa populations in peach orchards in Spain[J]. Phytopathology, 2007, 155(9): 549-556. doi: 10.1111/j.1439-0434.2007.01278.x
[31]	ZHOU Q X, HWANG S F, FU H T, et al. Genetic variation of Rhizoctonia solani isolates from canola in Alberta[J]. Canadian Journal of Plant Science, 2013, 94(4): 671-681. doi: 10.4141/cjps2012-114#.XXIPHfk6s7M
[32]	SHAH M D, VERMA K S, SINGH K, et al. Molecular diversity and gene flow among Botryodiplodia theobromae populations in pear using RAPD markers[J]. Applied Biological Research, 2012, 14(1): 39-46.
[33]	PUNJA Z K. Virulence of Chalara elegans on bean leaves, and host-tissue responses to infection[J]. Canadian Journal of Plant Pathology, 2004, 26(1): 52-62. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1080/07060660409507112
[34]	NYBOM H. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants[J]. Molecular Ecology, 2004, 13(5): 1143-1155. doi: 10.1111/j.1365-294X.2004.02141.x
[35]	CHEN C Q, HUANG L L, BUCHENOUER H, et al. Diversity among single zoospore isolates derived from single-zoosporangia of Phyrophthora sofae Kouf. and Gerd[J]. Journal of Phytopathology, 2009, 157(3): 181-187. doi: 10.1111/j.1439-0434.2008.01462.x

[1]	Chen Cun, Ding Changjun, Huang Qinjun, Zhang Jing, Liu Ning, Li Bo, Li Zhenghong, Su Xiaohua. Phenotypic and physiological trait diversity and population structure of Populus deltoides[J]. Journal of Beijing Forestry University, 2021, 43(6): 1-12. DOI: 10.12171/j.1000-1522.20200231
[2]	Qu Kai, Guo Haoping, Wang Baorui, Zhou Wenling, Hou Lili, Li Qin, Li Jihong, Cheng Tiantian. Genetic diversity analysis of Chionanthus retusus natural population based on SRAP molecular markers[J]. Journal of Beijing Forestry University, 2020, 42(12): 40-50. DOI: 10.12171/j.1000-1522.20200212
[3]	DAI Bo, DU Jin-you, YANG Jun-ming, HUANG Yin-ran, ZHANG Jun-ying. Construction of ISSR identification card of Ulmus pumila Jinye' and analysis of genetic diversity of 8 Ulmus materials.[J]. Journal of Beijing Forestry University, 2014, 36(3): 100-103. DOI: 10.13332/j.cnki.jbfu.2014.03.015
[4]	ZHANG Xiao-juan, SUN Zhen-yuan. ISSR analysis of Parthenocissus spp. and cultivars.[J]. Journal of Beijing Forestry University, 2011, 33(6): 177-180.
[5]	RUAN Zhen-yuan, YANG Han-qi, TIAN Bo, YANG Yu-ming, SUN Mao-sheng. Genetic diversity analysis based on ISSR among six populations of Dendrocalamus brandisii in Yunnan Province, China.[J]. Journal of Beijing Forestry University, 2010, 32(2): 46-51.
[6]	WANG Guo-xia, CAO Fu-liang, FANG Yan-ming. Genetic diversity of ancient male ginkgo trees by ISSR analysis[J]. Journal of Beijing Forestry University, 2010, 32(2): 39-45.
[7]	ZHANG Yu-rong, LUO Ju-chun, YU Jin-xiu. Genetic diversity of the endangered plant Abies ziyuanensis detected by ISSR markers[J]. Journal of Beijing Forestry University, 2007, 29(6): 41-46. DOI: 10.13332/j.1000-1522.2007.06.012
[8]	GUAN Ping, MA Dan-wei, WANG Gui-xia, SHI Jian-ming, CHEN Fang. ISSR analysis on genetic diversity of Gastrodia elata Bl.in Guizhou Province, southwestern China[J]. Journal of Beijing Forestry University, 2007, 29(6): 35-40. DOI: 10.13332/j.1000-1522.2007.06.003
[9]	JIN Ze-xin, LI Jun-min, LI Zeng-hong. ISSR analysis of the genetic diversity of Torreya jackii Chun natural populations in Xianju County, Zhejiang Province[J]. Journal of Beijing Forestry University, 2007, 29(1): 53-59. DOI: 10.13332/j.1000-1522.2007.01.010
[10]	CHEN Wei. Genetic diversity of the natural populations of Betula luminifera[J]. Journal of Beijing Forestry University, 2006, 28(6): 28-34.

Cited By

Cited by

Periodical cited type(3)

1.	唐丽，李春艳，贾文浩，刘振亚，李亚鹏，但红侠，张王斌. 新疆山桃腐烂病病原菌鉴定. 新疆农业科学. 2024(12): 3089-3096 .
2.	翟亚伟，陈虹瑾，刘楚丽，陈帅康，侯刘娟，马荣. 新疆杨树和柳树壳囊孢属真菌的生物学特性研究. 新疆农业大学学报. 2022(03): 205-213 .
3.	赵颖，马荣，尹永香，张志东，田呈明. 新疆不同来源金黄壳囊孢的多样性. 生物多样性. 2019(10): 1122-1131 .

Other cited types(10)

Get Citation

PDF

XML

Article views (2626) PDF downloads (48) Cited by(13)

Turn off MathJax

Article Contents

Abstract

References

Analysis of appearance features and genetic diversity of Cytospora chrysosperma causing poplar canker