Rust pathogen identification and mechanism of disease-resistance research on Kentucky bluegrass dwarf mutant

GAN Lu; SU Hao-tian; LING Xin-wen; YIN Shu-xia

doi:10.13332/j.1000-1522.20160315

Journal of Beijing Forestry University > 2017 > 39(3): 87-92. > DOI: 10.13332/j.1000-1522.20160315

GAN Lu, SU Hao-tian, LING Xin-wen, YIN Shu-xia. Rust pathogen identification and mechanism of disease-resistance research on Kentucky bluegrass dwarf mutant[J]. Journal of Beijing Forestry University, 2017, 39(3): 87-92. DOI: 10.13332/j.1000-1522.20160315

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Rust pathogen identification and mechanism of disease-resistance research on Kentucky bluegrass dwarf mutant

Institute of Turfgrass Science, Beijing Forestry University, Beijing, 100083, P. R. China

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Received Date: September 28, 2016
Revised Date: November 18, 2016
Published Date: February 28, 2017

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Abstract

Abstract

Rust is one of the destructive diseases on turfgrass. The species of rust pathogens are multiple and difficult to identify. In order to determine the rust pathogen species on Kentucky bluegrass wild type and dwarf mutant plant, pathogen morphology, along with ITS and β-tubulin gene sequence analysis were carried out. The analysis of ITS by BLAST and phylogenetic analysis of β-tubulin showed that the strains were related to Puccinia graminis of wheat, so we identified it as Puccinia graminis, which is the first report on Kentucky bluegrass in China. Moreover, transcriptional level of PR1L and NPR1L genes in Kentucky bluegrass inoculated with Puccinia graminis, as well as the expression of PRs protein, were investigated. The results presented that the expression of PR1L and NPR1L genes reached peak at 12 hours induced by Puccinia graminis, and the fold changes in dwarf mutant (A16) were 8.8 and 4.5, respectively, greater than in control wild type (WT). Besides, the expression of PRs in P. graminis infected plants 8 days later was higher than in non-infected plants. Defense mechanism of Kentucky bluegrass to rust was preliminary investigated and it presented the related disease-resistant gene and protein induced by rust pathogen. The research provides foundation for rust prevention and disease resistance breeding on Kentucky bluegrass in future.
- turfgrass,
- Kentucky bluegrass,
- dwarf mutant,
- rust pathogen identification,
- disease resistance

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References

[1]	BEARD J B, TOSHIKAZU T. Color atlas of turfgrass diseases[M]. Chelsea: Ann Arbor Press, 1997.
[2]	CPUCH H B. Diseases of turfgrasses[M]. New York: Reinhold Pub. Corp., 1962.
[3]	陈吉虎, 赵永军, 高旭彪, 等.北京地区冷季型草坪草病害调查及防治[J].草业科学, 2007, 24(12): 103-106. doi: 10.3969/j.issn.1001-0629.2007.12.025 CHEN J H, ZHAO Y J, GAO X B, et al. Investigation and control of cold-season turfgrass diseases in Beijing areas[J]. Pratacultural Science, 2007, 24(12): 103-106. doi: 10.3969/j.issn.1001-0629.2007.12.025
[4]	李春杰, 南志标.甘肃草坪草真菌病害初报[J].草业科学, 1998, 15(1): 49-51. http://www.cnki.com.cn/Article/CJFDTotal-CYKX801.011.htm LI C J, NAN Z B. Primary report on fungal diseases of turfgrass in Gansu Province, China[J]. Pratacultural Science, 1998, 15(1): 49-51. http://www.cnki.com.cn/Article/CJFDTotal-CYKX801.011.htm
[5]	薛福祥, 姚拓, 席琳乔.成都高尔夫球场草坪草主要病害病原鉴定及防治对策初报[J].草原与草坪, 2003(2): 23-25. doi: 10.3969/j.issn.1009-5500.2003.02.006 XUE F X, YAO T, XI L Q. Preliminary study on identification and control of turfgrass diseases in Chengdu golf course[J]. Grassland and Turf, 2003(2): 23-25. doi: 10.3969/j.issn.1009-5500.2003.02.006
[6]	薛福祥.兰州地区冷季型草坪真菌病害研究[J].草业科学, 2003, 20(3): 66-70. doi: 10.3969/j.issn.1001-0629.2003.03.019 XUE F X. Study on cold-season turfgrass diseases in Lanzhou rejoin[J]. Pratacultural Science, 2003, 20(3): 66-70. doi: 10.3969/j.issn.1001-0629.2003.03.019
[7]	文克俭, 尚以顺.贵州优良冷季型草坪主要病虫害防治技术[J].贵州畜牧兽医, 2003, 27(4): 34-36. doi: 10.3969/j.issn.1007-1474.2003.04.038 WEN K J, SHAN Y S. Study on pests and disease management control technology of cold-season turf in Guizhou rejoin[J]. Guizhou Animal Science and Veterinary Medicine, 2003, 27(4): 34-36. doi: 10.3969/j.issn.1007-1474.2003.04.038
[8]	刘金荣, 谢晓蓉.河西走廊草坪草病害调查[J].植物保护, 2002, 28(5): 39-40. doi: 10.3969/j.issn.0529-1542.2002.05.013 LIU J R, XIE X R. Investigation of turfgrass diseases in the Hexi Corridor[J]. Plant Protection, 2002, 28(5): 39-41. doi: 10.3969/j.issn.0529-1542.2002.05.013
[9]	侯玉霞, 石建宁.宁夏草坪草病害调查及防治[J].植物保护, 1999, 25(6): 28-29. doi: 10.3969/j.issn.0529-1542.1999.06.013 HOU Y X, SHI J N. Identification and control of turfgrass diseases in Ningxia[J]. Plant Protection, 1999, 25(6): 28-29. doi: 10.3969/j.issn.0529-1542.1999.06.013
[10]	张庆峰, 林树燕, 张强.南京市观赏型草坪草主要病害发生的特点及防治[J].江苏林业科技, 2005, 32(3): 26-28. doi: 10.3969/j.issn.1001-7380.2005.03.010 ZHANG Q F, LIN S Y, ZHANG Q. Prevention and control measures of main turfgrass diseases in Nanjing City[J]. Journal of Jiangsu Forestry Science & Technology, 2005, 32(3): 26-28. doi: 10.3969/j.issn.1001-7380.2005.03.010
[11]	侯丽冰, 贺伟, 刘小勇, 等.我国几种松干锈菌亲缘关系的ITS序列分析[J].北京林业大学学报, 2002, 24(5/6): 179-186. http://bjly.chinajournal.net.cn/WKC/WebPublication/paperDigest.aspx?paperID=87A21F4E-16FC-4BEE-90B9-3658AC3458C9 HOU L B, HE W, LIU X Y, et al. Sequence analysis of ITS region in relationships of Chinese isolates of Cronartium [J]. Journal of Beijing Forestry University, 2002, 24(5/6): 179-186. http://bjly.chinajournal.net.cn/WKC/WebPublication/paperDigest.aspx?paperID=87A21F4E-16FC-4BEE-90B9-3658AC3458C9
[12]	郑素娇.红小豆锈病菌鉴定和越冬及侵染过程的研究[D].大庆: 黑龙江八一农垦大学, 2015. http://cdmd.cnki.com.cn/Article/CDMD-10223-1015593026.htm ZHENG S J. Identification, overwintering and infection process of the pathogen causing adzuki bean rust[D]. Daqing: Heilongjiang Bayi Agricultural University, 2015. http://cdmd.cnki.com.cn/Article/CDMD-10223-1015593026.htm
[13]	代君丽, 于思勤, 韩帅, 等.一种从感病叶片中高效提取条锈菌基因组DNA的方法[J].西北农业学报, 2009, 18(2): 304-306. doi: 10.3969/j.issn.1004-1389.2009.02.068 DAI J L, YU S Q, HAN S, et al. An efficient protocol for isolatiing genomic DNA of Puccinia striiformis f. sp. tritici from infected leaves[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2009, 18(2): 304-306. doi: 10.3969/j.issn.1004-1389.2009.02.068
[14]	SOHPAL V K, DEY A, SINGH A. MEGA biocentric software for sequence and phylogenetic analysis: a review[J]. International Journal of Bioinformatics Research and Applications, 2010, 6(3): 230-240. doi: 10.1504/IJBRA.2010.034072
[15]	TIAN B, HARRISON R, MORTON J, et al. Proteomic analysis of sauvignon blanc grape skin, pulp and seed and relative quantification of pathogenesis-related proteins[J/OL]. PLoS One, 2015, 10(6): e130132[2016-05-04]. DOI: 10.1371/journal.pone.0130132.
[16]	CARUSO C, CHILOSI G, CAPORALE C, et al. Induction of pathogenesis-related proteins in germinating wheat seeds infected with Fusarium culmorum [J]. Plant Science, 1999, 140(1): 87-97. doi: 10.1016/S0168-9452(98)00199-X
[17]	ZAMBINO P J, SZABO L J. Phylogenetic relationships of selected cereal and grass rusts based on rDNA sequence analysis[J]. Mycologia, 1993, 85(3): 401-414. doi: 10.1080/00275514.1993.12026292
[18]	MA G B. Functional analysis of plant disease resistance genes and their downstream effectors.[J]. Current Opinion in Plant Biology, 1999, 2(4): 273-279. doi: 10.1016/S1369-5266(99)80049-1
[19]	DURRANT W E, DONG X. Systemic acquired resistance[J]. Annual Review of Phytopathology, 2004, 42(1): 185-209. doi: 10.1146/annurev.phyto.42.040803.140421
[20]	CAO H, GLAZEBROOK J, CLARKE J D, et al. The arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats[J]. Cell, 1997, 88(1): 57-63. doi: 10.1016/S0092-8674(00)81858-9
[21]	KINKEMA M, FAN W, DONG X. Nuclear localization of NPR1 is required for activation of PR gene expression[J]. The Plant Cell, 2000, 12(12): 2339-2350. doi: 10.1105/tpc.12.12.2339

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Rust pathogen identification and mechanism of disease-resistance research on Kentucky bluegrass dwarf mutant