Extraction technology of anthocyanin from Lonicera caerulea 'Beilei' fruit by ultrasonic-assisted countercurrent extraction

The response surface methodology based on single factor experiment was used to optimize processing parameters of extraction of Lonicera caerulea 'Beilei' anthocyanin by ultrasonic-assisted countercurrent extraction. The results showed that the optimal processing conditions of ultrasonic-assisted countercurrent extraction were ultrasonic time 28 min, extraction temperature 30 ℃ and ultrasonic power 120 W, the countercurrent extraction stage was three levels. The yields of anthocyanin under the optimal extraction conditions were up to (5.23±0.023) mg/g, the extraction rate was 99.80%, which was increased by 10% compared with ultrasonic extraction method. For the same batch of raw materials, compared with conventional ultrasonic extraction, this process can save three extraction solvent 67% and save time 44.4%.