Effects of shading and season on growth and accumulation of major secondary metabolites in <i>Cyclocarya paliurus</i>

DENG Bo; SHANG Xu-lan; LIU Gui-hua; XU Dan-dan; FANG Sheng-zuo

doi:10.13332/j.1000-1522.20170144

Journal of Beijing Forestry University > 2017 > 39(9): 66-75. > DOI: 10.13332/j.1000-1522.20170144

DENG Bo, SHANG Xu-lan, LIU Gui-hua, XU Dan-dan, FANG Sheng-zuo. Effects of shading and season on growth and accumulation of major secondary metabolites in Cyclocarya paliurus[J]. Journal of Beijing Forestry University, 2017, 39(9): 66-75. DOI: 10.13332/j.1000-1522.20170144

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Effects of shading and season on growth and accumulation of major secondary metabolites in Cyclocarya paliurus

1.
School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, Anhui, 230036, P.R. China
2.
College of Forestry, Nanjing Foestry University, Nanjing, Jiangsu, 210037, P.R. China

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Received Date: April 18, 2017
Revised Date: May 30, 2017
Published Date: August 31, 2017

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Abstract

Abstract

Cyclocarya paliurus is a multiple function plant, and has been traditionally used for drug formulations or ingredients of functional foods in China. Previous studies have shown that the leaves of C. paliurus contain a large number of physiological active compounds, such as flavonoids and triterpenoids, which were known to carry important physiological activity in humans. Understanding the variation patterns in secondary metabolites accumulation, and the relationship between secondary metabolism and growth along the environmental gradients can provide meaningful information for the standardized cultivation of medicinal artificial forest of C. paliurus. Shading treatment was used for studying the effects of shading treatments and sampling time on growth and accumulation of important secondary metabolites in C. paliurus from June to November. Furthermore, the relationship between secondary metabolism and growth of C. paliurus was analyzed. Our results indicated that tree height growth of C. paliurus was improved under one-layer shading, while basal diameter growth was significantly decreased under shading conditions. Accumulation of flavonoids, total triterpenoid, and cyclocarioside Ⅰ were significantly restrained by shading treatments. However, accumulation of other two individual triterpenoids cyclocaric acid B and arjunolic acid was improved by shading treatments. Under natural light condition, flavonoid contents in August and October were significant higher than other seasons, and showed a bimodal variation pattern. While, the fastigium of flavonoid accumulation in leaves of C. paliurus was delayed by shading treatment. Furthermore, under different shading conditions, the highest contents of total and individual triterpenoids were detected in September, and showed an unimodal variation pattern. A significant negative relationship between growth and accumulation of total triterpenoid and arjunolic acid was found through correlation analysis.
- Cyclocarya paliurus,
- light intensity,
- harvest season,
- flavonoid,
- triterpenoid

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References (46)

References

[1]	方升佐, 洑香香.青钱柳资源培育与开发利用的研究进展[J].南京林业大学学报(自然科学版), 2007, 31(1): 95-100. doi: 10.3969/j.issn.1000-2006.2007.01.023 FANG S Z, FU X X. Progress and prospects on silviculture and utilization of Cyclocarya paliurus resources[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2007, 31(1): 95-100. doi: 10.3969/j.issn.1000-2006.2007.01.023
[2]	方升佐, 杨万霞.青钱柳的开发利用与资源培育[J].林业科技开发, 2003, 17(1): 49-51. doi: 10.3969/j.issn.1000-8101.2003.01.019 FANG S Z, YANG W X. Silviculture and utilization of Cyclocarya paliurus resources[J]. China Forestry Science and Technology, 2003, 17(1): 49-51. doi: 10.3969/j.issn.1000-8101.2003.01.019
[3]	RADUSIENE J, KARPAVICIENE B, STANIUS Ž. Effect of external and internal factors on secondary metabolites accumulation in St. John's worth[J]. Botanica Lithuanica, 2012, 18(2): 101-108. doi: 10.2478/v10279-012-0012-8
[4]	鲁守平, 隋新霞, 孙群, 等.药用植物次生代谢的生物学作用及生态环境因子的影响[J].天然产物研究与开发, 2006, 18(6): 1027-1032. doi: 10.3969/j.issn.1001-6880.2006.06.037 LU S P, SUI X X, SUN Q, et al. Biological function of secondary metabolism of medicinal plants and influences of ecological environment[J]. Natural Production Research and Development, 2006, 18(6): 1027-1032. doi: 10.3969/j.issn.1001-6880.2006.06.037
[5]	王洋, 戴绍军, 闫秀峰.光强对喜树幼苗喜树碱含量及分配的影响[J].生态学报, 2004, 24(6): 260-264. http://d.old.wanfangdata.com.cn/Periodical/hljdxzrkxxb200602030 WANG Y, DAI S J, YAN X F. Effects of light intensity on camptothecin content and allocation in Camtotheca acuminate seedlings[J]. Acta Ecologica Sinica, 2004, 24(6): 260-264. http://d.old.wanfangdata.com.cn/Periodical/hljdxzrkxxb200602030
[6]	JOSEP P, JOAN L. Effects of carbon dioxide, water supply, and seasonally on terpene content and emission by Rosmarinus officinalis[J]. Journal of Chemical Ecology, 1997, 23(4): 979-993. doi: 10.1023/B:JOEC.0000006383.29650.d7
[7]	SHELTON A L. Variable chemical defences in plants and their effects on herbivore behavior[J]. Evolutionary Ecology Research, 2000, 2(2): 231-249.
[8]	CAI Z Q, WANG W H, YANG J, et al. Growth, photosynthesis and root reserpine concentrations of two Rauvolfia species in response to a light gradient[J]. Industrial Crops and Products, 2009, 30(2): 220-226. doi: 10.1016/j.indcrop.2009.03.010
[9]	FANG S Z, YANG W X, CHU X L, et al. Provenance and temporal varations in selected flavonoids in leaves of Cyclocarya paliurus[J]. Food Chemistry, 2011, 124(4): 1382-1386. doi: 10.1016/j.foodchem.2010.07.095
[10]	SYLWIA G, LESZCZYNSKI B, WIESLAW O. Effect of low and high-saponin lines of alfalfa on pea aphid[J]. Journal of Insect Physiology, 2006, 52(7): 737-743. doi: 10.1016/j.jinsphys.2006.04.001
[11]	FANG S Z, WANG J Y, WEI Z Y, et al. Methods to break seed dormancy in Cyclocarya paliurus (Batal) Iljinskaja[J]. Scientia Horticulturae, 2006, 110(3): 305-309. doi: 10.1016/j.scienta.2006.06.031
[12]	HUANG W, XUE A, NIU H, et al. Optimised ultrasonic-assisted extraction of flavonoids from Folium eucommiae and evaluation of antioxidant activity in multi-test systems in vitro[J]. Food Chemistry, 2009, 114(3): 1147-1154. doi: 10.1016/j.foodchem.2008.10.079
[13]	BAO J S, CAI Y, SUN M, et al. Anthocyanins, flavonols, and free radical scavenging activity of Chinese bayberry (Myrica rubra) extracts and their color properties and stability[J]. Journal of Agricultural and Food Chemistry, 2005, 52(6): 2327-2332. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=483fc5684de84398c616ce63c4d2d339
[14]	FAN J P, HE C H. Simultaneous quantification of three major bioactive triterpene acids in the leaves of Diospyros kaki by high-performance liquid chromatography method[J]. Journal of Pharmaceutical and Biomedical Analysis, 2006, 41(3): 950-956. doi: 10.1016/j.jpba.2006.01.044
[15]	LV J, LU Y J, NIU Y G, et al. Effect of genotype, environment, and their interaction on phytochemical compositions and antioxidant properties of soft winter wheat flour[J]. Food Chemistry, 2013, 138(1): 454-462. doi: 10.1016/j.foodchem.2012.10.069
[16]	SZAKIEL A, PACZKOWSKI C, HENRY M. Influence of environmental abiotic factors on the content of saponins in plants[J]. Phytochemistry Review, 2011, 10(4): 471-491. doi: 10.1007/s11101-010-9177-x
[17]	SCHMIDT S, ZIETZ M, SCHREINER M, et al. Genotypic and climatic influence on the concentration and composition of flavonoids in kales (Brassica oleracea var. sabellica)[J]. Food Chemistry, 2010, 119(4): 1293-1299. doi: 10.1016/j.foodchem.2009.09.004
[18]	POUTARAUD A, GIRARDIN P. Influence of chemical characteristics of soil on mineral and alkaloid seed contents of Colchicum autumnale[J]. Environmental and Experimental Botany, 2005, 54(2): 101-108. doi: 10.1016/j.envexpbot.2004.06.007
[19]	DENG B, SHANG X L, FANG S Z, et al. Integrated effects of light intensity and fertilization on growth and flavonoid accumulation in Cyclocarya paliurus[J]. Journal of Agriculture and Food Chemistry, 2012, 60(25): 6286-6292. doi: 10.1021/jf301525s
[20]	CRONIN G, LODGE D M. Effects of light and nutrient availability on the growth, allocation, carbon/nitrogen balance, phenolic chemistry and resistance to herbivory of two freshwater macrophytes[J]. Oecologis, 2003, 137(1): 32-41. doi: 10.1007/s00442-003-1315-3
[21]	CHAREST P M, BRISSON L, IBRAHIM R K. Ultrastructural feature of flavonoid accumulation in leaf cells[J]. Protoplasma, 1986, 134(2): 95-101.
[22]	RYAN K G, MARKHAM K R, BLOOR S J, et al. UV-B radiation induces increase in quercetin: kaempferol ratio in wild-type and transgenic lines of Petunia[J]. Photochemistry and Photobiology, 1998, 68(3): 323-330. doi: 10.1111/j.1751-1097.1998.tb09689.x
[23]	BURRITT D J, MACKENZIE S. Antioxidant metabolism during acclimation of begonia×erythrophylla to high light levels[J]. Annals of Botany, 2003, 91(7): 783-794. doi: 10.1093/aob/mcg076
[24]	AGATI G, STEFANO G, BIRICOLTI S, et al. Mesophyll distribution of 'antioxidant' flavonoid glycosides in Ligustrum vulgare leaves under contrasting sunlight irradiance[J]. Annuals of Botany, 2009, 104(5): 853-861. doi: 10.1093/aob/mcp177
[25]	JAAKOLA L, MAATTA-RⅡHINEN K, KARENLAMPI S, et al. Activation of flavonoid biosynthesis by solar radiation in bilberry (Vaccinium myrtillus L.) leaves [J]. Planta, 2004, 218(5): 721-728. doi: 10.1007/s00425-003-1161-x
[26]	KLAPPER R, FRANLEL S, BERENBAUM M R. Anthocyanin content and UVB ensitivity in Brassica rapa[J]. Photochemical and Photobiology Sciences, 1996, 63(6): 811-813. doi: 10.1111/j.1751-1097.1996.tb09635.x
[27]	REUBER S, BORNMAN J F, WEISSENBOCK G. A flavonoid matant of barley (Hordeum vulgare L.) exhibits increased sensitivity to UVB radiation in the primary leaf[J]. Plant Cell and Environment, 1996, 19: 593-601. doi: 10.1111/j.1365-3040.1996.tb00393.x
[28]	REICHARDT P B, CHAPIN F S, BRYANT J P, et al. Carbon/nutrient balance as a predictor of plant defense in Alaskan balsam poplar: potential importance of metabolite turnover[J]. Oecologia, 1991, 88(3): 401-406. doi: 10.1007/BF00317585
[29]	SZAKIEL A, PACZKOWSKI C, HENRY M. Influence of environmental abiotic factors on the content of saponins in plants[J]. Phytochemistry Review, 2011, 10(4): 471-491. doi: 10.1007/s11101-010-9177-x
[30]	HOULE A, CHAPMAN C A, VICKERY W L. Intratree variation in fruit production and implications for Primate foraging[J]. International Journal of Primatology, 2007, 28(6): 1197-1217. doi: 10.1007/s10764-007-9214-9
[31]	FOURNIER A R, PROCTOR J T A, GAUTHIER L, et al. Understory light and root ginsenosides in forest-grown Panax quinquefolius[J]. Phytochemistry, 2003, 63(7): 777-782. doi: 10.1016/S0031-9422(03)00346-7
[32]	SHIMOYAMADA M, OKUBO K. Variation in saponin contents in germinating soybean seeds and effect of light irradiation[J]. Agricultural and Biological Chemistry, 1991, 55(2): 577-579. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1080/00021369.1991.10870593
[33]	TANSAKUL P, SHIBUYA M, KUSHIRO T, et al. Dammarenediol-Ⅱ synthase, the first dedicated enzyme for ginsenoside biosynthesis, in Panax ginseng[J]. FEBS Letters, 2006, 580(22): 5143-5149. doi: 10.1016/j.febslet.2006.08.044
[34]	AMARAL J S, SEABRA R M, ANDRADE P B, et al. Phenolic profile in the quality control of walnut (Juglans regia L.) leaves[J]. Food Chemistry, 2004, 88(3): 373-379. doi: 10.1016/j.foodchem.2004.01.055
[35]	VOGT T, GULZ P G. Accumulation of flavonoids during leaf development in Citrus laurifolius[J]. Phytochemistry, 1994, 36(3): 591-597. doi: 10.1016/S0031-9422(00)89780-0
[36]	IWASHINA T. The structure and distribution of the flavonoids in plants[J]. Journal of Plant Research, 2000, 113: 287-299. doi: 10.1007/PL00013940
[37]	CAMARGO M G G, SOUZA R M, REYS P, et al. Effects of environmental conditions associated to the cardinal orientation on the reproductive phenology of the cerrado savanna tree Xylopia aromatica (Annonaceae)[J]. Annals of the Brazillian Academy of Science, 2011, 83(3): 1007-1019. doi: 10.1590/S0001-37652011005000014
[38]	ALQAHTANI A, TONGKAO-ON W, LI K M, et al. Evaluation of regional and seasonal variations of triterpene and flavonoid contents in Centella asiatica by HPLC-DAD[J]. Planta Medica, 2011, 77(5): 91-92.
[39]	TAVA A, ODOARDI M, OLESZEK W. Seasonal changes of saponin content in five alfalfa (Medicago sativa) cultivars[J]. Agriculture and Medicine, 1999, 129: 111-116. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=62c17d5697273e7ef676658928d1ce0d
[40]	PECETTI L, TAVA A, ROMANI M, et al. Variety and environmental effects on the dynamics of saponins in lucerne (Medicago sativa L.)[J]. European Journal of Agronomy, 2006, 25(3): 187-192. doi: 10.1016/j.eja.2006.04.013
[41]	LIN J T, CHEN S L, LIU S C, et al. Effect of harvest time on saponins in Yam (Dioscorea pseudojaponica Yamamoto)[J]. Journal of Food and Drug Analysis, 2009, 17(2): 116-122. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=7e42f3f24ae3a7698e56b2f53cef3e5c
[42]	TENG H M, FANG M F, CAI X, et al. Localization and dynamic change of saponin in vegetative organs of Polygala tenuifolia[J]. Journal of Integrative Plant Biology, 2009, 51(6): 529-536. doi: 10.1111/j.1744-7909.2009.00830.x
[43]	司徒琳莉, 袁长友.次生代谢物:代谢途径、分类、作用及其生产(Ⅰ)[J].牡丹江师范学院学报(自然科学版), 2001(3): 11-15. doi: 10.3969/j.issn.1003-6180.2001.03.006 SITU L L, YUAN C Y. The metabolic pathway, classification, function and its production of secondary metabolites in plants (Ⅰ)[J]. Learned Journal of Mudanjiang Teachers College (Natural Sciences Edition), 2001(3): 11-15. doi: 10.3969/j.issn.1003-6180.2001.03.006
[44]	STAMP N. Out of the quagmire of plant defense hypotheses[J]. The Quarterly Review of Biology, 2003, 78(1): 23-55. doi: 10.1086/367580
[45]	HORNER J D, GOSZ J R, CATES R G. The role of carbon-based plant secondary metabolites in decomposition in terrestrial ecosystems[J]. The American Naturalist, 1988, 132(6): 869-883. doi: 10.1086/284894
[46]	WEIS A E, SIMMS E L, HOCHBERG M E. Will plants and tolerance be genetically correlated? Effects of intrinsic growth rate and self-limitation on regrowth[J]. Evolutionary Ecology Research, 2000, 14(4): 331-352. doi: 10.1023/A%3A1010950932468

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Effects of shading and season on growth and accumulation of major secondary metabolites in Cyclocarya paliurus

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