Effects of LdNPV on growth, development and biochemical enzymatic activities of <i>Lymantria dispar</i> under CO<sub>2</sub> concentration stress

Li Xinyue; Wang Zhenyue; Cao Chuanwang

doi:10.13332/j.1000-1522.20180199

Journal of Beijing Forestry University > 2019 > 41(9): 139-146. > DOI: 10.13332/j.1000-1522.20180199

Li Xinyue, Wang Zhenyue, Cao Chuanwang. Effects of LdNPV on growth, development and biochemical enzymatic activities of Lymantria dispar under CO₂ concentration stress[J]. Journal of Beijing Forestry University, 2019, 41(9): 139-146. DOI: 10.13332/j.1000-1522.20180199

Citation:

PDF (711 KB)

Effects of LdNPV on growth, development and biochemical enzymatic activities of Lymantria dispar under CO₂ concentration stress

School of Forestry, Northeast Forestry University, Harbin 150040, Heilongjiang, China

More Information

Received Date: June 19, 2018
Revised Date: September 20, 2018
Available Online: July 12, 2019
Published Date: August 31, 2019

Graphical Abstract

Abstract

Abstract

ObjectiveIn this study, the forest pest Lymantria dispar was used to study the effects of LdNPV on the development, detoxifying enzymes and protective enzymes of Lymantria dispar larvae under CO₂ concentration stress.
MethodThe effects of LdNPV on the growth and development of L. dispar larvae under different CO₂ concentrations (397, 550 and 750 μL/L) reared into the closed CO₂ artificial climate chamber, and in vivo detoxifying enzymes and protective enzyme activities of L. dispar larvae were also determined by spectrophotometry.
Result The cumulative growth rate of fresh mass of the 3rd instar larvae of L. dispar decreased under high CO₂ concentration stress. The cumulative increase rates of fresh mass of the 3rd instar L. dispar larvae infected by LdNPV were higher than the control by 81.27%, 71.63% and 68.41%, respectively. The mortality rate increased with increasing CO₂ concentration, and the cumulative mortality rate of 750 μL/L CO₂ treatment was 27.09%. Under the high CO₂ concentration stress, the activities of detoxifying enzymes CarE and AChE in the 3rd instar larvae of L. dispar were induced with the increase of CO₂ concentration, and the ALP activity was inhibited with the increase of CO₂ concentration; the activity of protective enzyme CAT was induced with the increase of CO₂ concentration while SOD activity was inhibited with increasing CO₂ concentration. However, the activities of CarE, ALP, AChE and CAT in 3rd instar larvae of L. dispar infected by LdNPV were inhibited with the increase of CO₂ concentration, and the activity of SOD was induced with the increase of CO₂ concentration.
ConclusionThe duration and cumulative mortality rate of L. dispar larvae infected by LdNPV increased while cumulative growth rate decreased after different CO₂ concentraion treatments. The activities of CarE, AChE, ALP and CAT in 3rd instar larvae of L. dispar decreased with the increase of both the treatment time and CO₂ concentration, and the activities of SOD were increased. Therefore, elevated concentration of CO₂ may increase the virulence of LdNPV to the L. dispar larvae by affecting the growth, development and biochemical enzymes.
- Lymantria dispar,
- CO₂ concentration,
- LdNPV,
- growth and development,
- detoxifying enzyme,
- protective enzyme

FullText(HTML)

References (28)

References

[1]	戈峰, 陈法军. 大气CO₂浓度增加对昆虫的影响[J]. 生态学报, 2006, 26(3):935−944. doi: 10.3321/j.issn:1000-0933.2006.03.040 Ge F, Chen F J. Impacts of elevated CO₂ on insects[J]. Acta Ecologica Sinica, 2006, 26(3): 935−944. doi: 10.3321/j.issn:1000-0933.2006.03.040
[2]	Smith P H D, Jones T H. Effects of elevated CO₂ on the chrysanthemum leaf-miner, Chromatomyia syngenesiae: a greenhouse study[J]. Global Change Biology, 2010, 4(3): 287−291.
[3]	Johns C V, Hughes L. Interactive effects of elevated CO₂ and temperature on the leaf-miner Dialectica scalariella Zeller (Lepidoptera: Gracillariidae) in Paterson ’s curse, Echium plantagineum (Boraginaceae)[J]. Global Change Biology, 2002, 8(2): 142−152. doi: 10.1046/j.1365-2486.2002.00462.x
[4]	Klironomos J N, Allen M F, Rilling M C, et al. Abrupt rise in atmospheric CO₂ overestimates community response in a model plant-soil system[J]. Nature, 2005, 433: 621−624. doi: 10.1038/nature03268
[5]	Brooks G L, Whittaker J B. Responses of three generations of a xylem-feeding insect, Neophilaenus lineatus (Homoptera), to elevated CO₂[J]. Global Change Biology, 1999, 5: 395−401. doi: 10.1046/j.1365-2486.1999.00239.x
[6]	高慧璟. 高CO₂环境下转Bt棉花的氮素代谢生理及其非靶标抗虫性研究[D]. 南京: 南京农业大学, 2011. Gao H J. Effects of elevated CO₂ on nitrogen metabolism and non-targrt resistance of transgenic Bt cottom[D]. Nanjing: Nanjing Agricultural University, 2011.
[7]	庄静. 递增与倍增CO₂浓度对多代褐飞虱生活史及生化物质的影响[D]. 武汉: 华中农业大学, 2014. Zhuang J. Effect of gradual and double increasing CO₂ concentrations on the life history and biochemical substances in multiple generations of Nilaparvata lugens[D]. Wuhan: Huazhong Agricultural University, 2014.
[8]	刘建业, 钱蕾, 蒋兴川, 等. CO₂浓度升高对西花藓马和花藓马成虫体内解毒酶和保护酶活性的影响[J]. 昆虫学报, 2014, 57(7):754−761. Liu J Y, Qian L, Jiang X C, et al. Effects of elevated CO₂ concentration on the activities of detoxifying enzymes and protective enzymes in adults of Frankliniella occidentalis and F. intonsa (Thysanoptera: Thripidae)[J]. Acta Entomologica Sinica, 2014, 57(7): 754−761.
[9]	陈立坤, 张永安, 王玉珠, 等. Bt和LdNPV混用对舞毒蛾幼虫毒杀效果的研究[J]. 中国森林病虫, 2013, 32(3):36−39. doi: 10.3969/j.issn.1671-0886.2013.03.010 Chen L K, Zhang Y A, Wang Y Z, et al. Cytotoxic effect of Bacillus thuringiensis combined with Lymantria dispar nuclear polyhedrosis virus on gypsy moth larvae[J]. Forest Pest and Disease, 2013, 32(3): 36−39. doi: 10.3969/j.issn.1671-0886.2013.03.010
[10]	周建华, 胡应之, 肖育贵, 等. 荧光增白剂Tinapal LPW对蜀柏毒蛾核型多角体病毒增效作用研究[J]. 中国病毒学, 2006, 32(3):273−276. Zhou J H, Hu Y Z, Xiao Y G, et al. Study on a synergistic effect of Tinopal LPW on Parocneria orienta NPV[J]. Virologica Sinica, 2006, 32(3): 273−276.
[11]	朱丽春, 段立清, 特木钦, 等. 舞毒蛾核型多角体病毒3个地理品系的毒力比较[J]. 中国森林病虫, 2008, 27(5):4−6. doi: 10.3969/j.issn.1671-0886.2008.05.002 Zhu L C, Duan L Q, Te M Q, et al. Virulence comparison among three geographic strains of LdNPV[J]. Forest Pest and Disease, 2008, 27(5): 4−6. doi: 10.3969/j.issn.1671-0886.2008.05.002
[12]	刘海晶, 段立清, 李海平, 等. 绿原酸提高舞毒蛾核型多角体病毒(LdNPV)的致病力[J]. 昆虫学报, 2016, 59(5):568−572. Liu H J, Duan L Q, Li H P, et al. Chlorogenic acid enhances the virulence of Lymantria dispar necleopolyhydrovirus(LdNPV)[J]. Acta Entomologica Sinica, 2016, 59(5): 568−572.
[13]	Van Asperen K. A study of housefly esterases by means of a sensitive colorimetric method[J]. Journal of Insect Physiology, 1962, 8: 401−416. doi: 10.1016/0022-1910(62)90074-4
[14]	Bessey O A, Lowry O H, Brock M J. A method for the rapid determination of alkaline phosphates with five cubic millimeters of serum[J]. Journal of Biological Chemistry, 1946, 164(1): 321−329.
[15]	Gorun V, Proinov I, Baltescu V, et al. Modified Ellman procedure for assay of cholinesterases in crude enzymatic preparations[J]. Analytical Biochemistry, 1978, 86(1): 324−326. doi: 10.1016/0003-2697(78)90350-0
[16]	Cohen G P, Dembieck D, Marcus J, et al. Measurement of catalase activity in tissue extracts[J]. Analytical Biochemistry, 1970, 34: 30−38. doi: 10.1016/0003-2697(70)90083-7
[17]	Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamidae gels[J]. Analytical Biochemistry, 1971, 44: 276−287. doi: 10.1016/0003-2697(71)90370-8
[18]	Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding[J]. Analytical Biochemistry, 1976, 72: 248−254. doi: 10.1016/0003-2697(76)90527-3
[19]	戈峰. 应对全球气候变化的昆虫学研究[J]. 应用昆虫学报, 2011, 48(5):1117−1122. doi: 10.7679/j.issn.2095-1353.2011.185 Ge F. Challenges facing entomologists in a changing global climate[J]. Chinese Journal of Applied Entomology, 2011, 48(5): 1117−1122. doi: 10.7679/j.issn.2095-1353.2011.185
[20]	吴刚, 陈法军, 戈峰. CO₂浓度升高对棉铃虫生长发育和繁殖的直接影响[J]. 生态学报, 2006, 26(6):1733−1738. Wu G, Chen F J, Ge F. Direct effects of elevated CO₂ on growth, development and reproduction of cotton bollworm Helicoverpa armigera Hǜbner[J]. Acta Ecologica Sinica, 2006, 26(6): 1733−1738.
[21]	Duan L, Otvos I S. Influence of larval age and virus concentration on mortality and sublethal effects of a nucleopolyhedrovirus on the western spruce budworm (Lepidoptera: Tortricidae)[J]. Environmental Entomology, 2001, 30(1): 136−146. doi: 10.1603/0046-225X-30.1.136
[22]	席景会, 潘洪玉, 刘伟成, 等. 八字地老虎核型多角体病毒对宿主的弱化作用[J]. 昆虫天敌, 2000, 22(2):75−78. doi: 10.3969/j.issn.1674-0858.2000.02.006 Xi J H, Pan H Y, Liu W C, et al. The debilitation of AnNPV on spotted cutworm (Lepid: Noctuidae)[J]. Natural Enemies of Insects, 2000, 22(2): 75−78. doi: 10.3969/j.issn.1674-0858.2000.02.006
[23]	苑冉, 李欣悦, 吴韶平, 等. CO₂浓度升高对舞毒蛾生长发育和体内解毒酶及保护酶活性的影响[J]. 林业科学, 2018, 54(12):102−109. doi: 10.11707/j.1001-7488.20181211 Yuan R, Li X Y, Wu S P, et al. Effects of elevated CO₂ concentration on growth and development of Lymantria dispar (Lepidoptera: Lymantridae), as well as on activities of detoxifying enzymes and protective enzymes in the body[J]. Scientia Silvae Sinicae, 2018, 54(12): 102−109. doi: 10.11707/j.1001-7488.20181211
[24]	薛英伟, 涂增, 万永继, 等. 用柘树叶饲养家蚕对蚕体生长发育及3种代谢酶活性的影响[J]. 蚕业科学, 2009, 35(2):408−411. doi: 10.3969/j.issn.0257-4799.2009.02.032 Xue Y W, Tu Z, Wan Y J, et al. Effects of tricuspid cudranis leaves on growth and development and activity of three metabolic enzymes in the silkworm (Bombyx mori)[J]. Canye Kexue, 2009, 35(2): 408−411. doi: 10.3969/j.issn.0257-4799.2009.02.032
[25]	李龙, 张蕾, 张东阳, 等. 接种BmNPV后抗性品种和非抗性品种家蚕组织中2种酶活性的比较研究[J]. 安徽农业科学, 2015, 43(11):123−126. doi: 10.3969/j.issn.0517-6611.2015.11.047 Li L, Zhang L, Zhang D Y, et al. Comparative studies on the activities of two kinds of enzyme in the tissues of resistant breed and control breed Bombyx mori inoculated with BmNPV[J]. Journal of Anhui Agricultural Sciences, 2015, 43(11): 123−126. doi: 10.3969/j.issn.0517-6611.2015.11.047
[26]	于欢, 吕婷婷, 李晓峰, 等. HearNPV侵染对棉铃虫中场碱性磷酸酶活性及表达水平的影响[J]. 西北农林科技大学学报(自然科学版), 2012, 40(12):127−130. Yu H, Lü T T, Li X F, et al. Effects of HearNPV infection on the enzyme activity and gene expression of alkaline phosphatase from midgut of Helicoverpa armigera[J]. Journal of Northwest A&F University (Natural Science Edition), 2012, 40(12): 127−130.
[27]	李周直, 沈惠娟, 蒋巧根, 等. 几种昆虫体内保护酶系统活力的研究[J]. 昆虫学报, 1994, 26(6):1732−1738. Li Z Z, Shen H J, Jiang Q G, et al. A study on the activities of endogenous enzymes of protective system in some insects[J]. Acta Entomologica Siniga, 1994, 26(6): 1732−1738.
[28]	石生林, 张涛, 李群, 等. ApNPV侵染对柞蚕蛹体内保护酶活性的影响[J]. 沈阳农业大学学报, 2008, 39(5):625−627. doi: 10.3969/j.issn.1000-1700.2008.05.026 Shi S L, Zhang T, Li Q, et al. Effects of ApNPV infection on superoxide dismutase, catalase and phenoloxidase of Antheraea pernyi pupa[J]. Journal of Shenyang Agricultural University, 2008, 39(5): 625−627. doi: 10.3969/j.issn.1000-1700.2008.05.026

[1]	Zhang Luyue, Liu Yanhong, Han Dongqing. Differences in growth and adaptive strategies between male and female plants of Cercidiphyllum japonicum[J]. Journal of Beijing Forestry University, 2024, 46(12): 71-81. DOI: 10.12171/j.1000-1522.20230263
[2]	Liu Xiao, Yu Zhiming, Zhang Yang, Guo Jin, Zeng Guangchen. Evaluation of heat transfer performance of engineered wood flooring with built-in electric heating semi-conductive layer[J]. Journal of Beijing Forestry University, 2023, 45(5): 155-162. DOI: 10.12171/j.1000-1522.20220503
[3]	Liu Di, Sun Caowen, Wang Lixian, Jia Liming. Evaluation on field heat resistance of Sorbus pohuashanensis clonal seedlings introduced from different regions[J]. Journal of Beijing Forestry University, 2020, 42(4): 21-31. DOI: 10.12171/j.1000-1522.20190266
[4]	Liu Jingjing, Ma Lan, Li Junyou, Chen Peiyan, Zhang Jinge, Sun Zhanwei, Yan Lin, Zhang Dong. Experimental study on hydrodynamic characteristics of overland flow under different resistance conditions[J]. Journal of Beijing Forestry University, 2019, 41(8): 115-123. DOI: 10.13332/j.1000-1522.20190070
[5]	XU Kang, L Jian-xiong, LI Xian-jun, WU Yi-qiang. Effect of heat treatment on dimensional stability of phenolic resin impregnated poplar wood.[J]. Journal of Beijing Forestry University, 2015, 37(9): 70-77. DOI: 10.13332/j.1000-1522.20150019
[6]	WANG Yan-ping, LIU Mei-qin, SHI Jing, LIU Sheng-li, CHEN Yu-zhen, LU Cun-fu. Enhanced tolerance against heat stress of Escherichia coli cells by overexpressing an Ammopiptanthus mongolicus heat stress associated protein gene AmHsa32.[J]. Journal of Beijing Forestry University, 2012, 34(5): 37-43.
[7]	LI Yan-jun, TANG Rong-qiang, BAO Bin-fu, SUN Hui. Mechanical properties and dimensional stability of heat-treated Chinese fir[J]. Journal of Beijing Forestry University, 2010, 32(4): 232-236.
[8]	HUANG Rong-feng, Lv Jian-xiong, CAO Yong-jian, ZHAO Xiu, ZHAO You-ke, ZHOU Yong-dong, WU Yu-zhang. Impact of heat treatment on chemical composition of Chinese white poplar wood.[J]. Journal of Beijing Forestry University, 2010, 32(3): 155-160.
[9]	WANG Guo-xia, CAO Fu-liang, FANG Yan-ming. Genetic diversity of ancient male ginkgo trees by ISSR analysis[J]. Journal of Beijing Forestry University, 2010, 32(2): 39-45.
[10]	SUN Xiao-xia, XIE Xiang-ming, WU Yu-ying, LIU Ya-jie, HE Xiao-qing. Induction of xylanase from Strepotmyces albus and analysis of thermostability and alkali-tolerance[J]. Journal of Beijing Forestry University, 2005, 27(3): 72-75.

Cited By

Cited by

Periodical cited type(1)

陈铭，郭琳，郑笑，姜明云，王茹，丁雨龙，高志民，魏强. 中国15个主产区毛竹纤维形态比较. 南京林业大学学报(自然科学版). 2018(06): 7-12 .

Other cited types(2)

Get Citation

PDF

XML

Article views (2779) PDF downloads (40) Cited by(3)

Turn off MathJax

Article Contents

Abstract

References

Effects of LdNPV on growth, development and biochemical enzymatic activities of Lymantria dispar under CO₂ concentration stress