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Li Xinyue, Wang Zhenyue, Cao Chuanwang. Effects of LdNPV on growth, development and biochemical enzymatic activities of Lymantria dispar under CO2 concentration stress[J]. Journal of Beijing Forestry University, 2019, 41(9): 139-146. DOI: 10.13332/j.1000-1522.20180199
Citation: Li Xinyue, Wang Zhenyue, Cao Chuanwang. Effects of LdNPV on growth, development and biochemical enzymatic activities of Lymantria dispar under CO2 concentration stress[J]. Journal of Beijing Forestry University, 2019, 41(9): 139-146. DOI: 10.13332/j.1000-1522.20180199

Effects of LdNPV on growth, development and biochemical enzymatic activities of Lymantria dispar under CO2 concentration stress

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  • Received Date: June 19, 2018
  • Revised Date: September 20, 2018
  • Available Online: July 12, 2019
  • Published Date: August 31, 2019
  • ObjectiveIn this study, the forest pest Lymantria dispar was used to study the effects of LdNPV on the development, detoxifying enzymes and protective enzymes of Lymantria dispar larvae under CO2 concentration stress.
    MethodThe effects of LdNPV on the growth and development of L. dispar larvae under different CO2 concentrations (397, 550 and 750 μL/L) reared into the closed CO2 artificial climate chamber, and in vivo detoxifying enzymes and protective enzyme activities of L. dispar larvae were also determined by spectrophotometry.
    Result The cumulative growth rate of fresh mass of the 3rd instar larvae of L. dispar decreased under high CO2 concentration stress. The cumulative increase rates of fresh mass of the 3rd instar L. dispar larvae infected by LdNPV were higher than the control by 81.27%, 71.63% and 68.41%, respectively. The mortality rate increased with increasing CO2 concentration, and the cumulative mortality rate of 750 μL/L CO2 treatment was 27.09%. Under the high CO2 concentration stress, the activities of detoxifying enzymes CarE and AChE in the 3rd instar larvae of L. dispar were induced with the increase of CO2 concentration, and the ALP activity was inhibited with the increase of CO2 concentration; the activity of protective enzyme CAT was induced with the increase of CO2 concentration while SOD activity was inhibited with increasing CO2 concentration. However, the activities of CarE, ALP, AChE and CAT in 3rd instar larvae of L. dispar infected by LdNPV were inhibited with the increase of CO2 concentration, and the activity of SOD was induced with the increase of CO2 concentration.
    ConclusionThe duration and cumulative mortality rate of L. dispar larvae infected by LdNPV increased while cumulative growth rate decreased after different CO2 concentraion treatments. The activities of CarE, AChE, ALP and CAT in 3rd instar larvae of L. dispar decreased with the increase of both the treatment time and CO2 concentration, and the activities of SOD were increased. Therefore, elevated concentration of CO2 may increase the virulence of LdNPV to the L. dispar larvae by affecting the growth, development and biochemical enzymes.
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