Enhancement of embryogenic callus proliferation in Chinese pine (<i>Pinus tabuliformis</i>) by airlift bioreactor

Li Yixuan; Zhao Jian; Fu Shuangbin; Dong Mingliang; Yang Shuo; Li Shanshan; Kong Lisheng; Zhang Jinfeng

doi:10.13332/j.1000-1522.20190221

Journal of Beijing Forestry University > 2019 > 41(11): 37-43. > DOI: 10.13332/j.1000-1522.20190221

Li Yixuan, Zhao Jian, Fu Shuangbin, Dong Mingliang, Yang Shuo, Li Shanshan, Kong Lisheng, Zhang Jinfeng. Enhancement of embryogenic callus proliferation in Chinese pine (Pinus tabuliformis) by airlift bioreactor[J]. Journal of Beijing Forestry University, 2019, 41(11): 37-43. DOI: 10.13332/j.1000-1522.20190221

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Enhancement of embryogenic callus proliferation in Chinese pine (Pinus tabuliformis) by airlift bioreactor

1.
National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants Biological Engineering of State Forestry Administration, School of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
2.
Shenzhou Lüpeng Agricultural Technology, Beijing 101105, China
3.
Department of Biology, University of Victoria, Victoria BC V8P5C2, Canada

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Received Date: May 09, 2019
Revised Date: June 02, 2019
Available Online: November 01, 2019
Published Date: October 31, 2019

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Abstract

Abstract

ObjectiveThe proliferation of a great number of active embryogenic callus can provide sufficient materials for somatic embryogenesis and plant regeneration of Pinus tabuliformis. However, the yield of flask suspension proliferation is limited and easy acidification necrosis. The establishment of airlift bioreactor system for embryogenic callus multiplication of P. tabuliformis can promote the proliferation of embryogenic callus of P. tabuliformis.
MethodIn this study, P. tabuliformis callus was used as material, and L₉ (3⁴) orthogonal design was used to investigate the effects of three factors on callus proliferation in bioreactor, including inoculation volume, ratio of old and new media and hormone concentration. Then the suspension culture in conical flask was compared with that in conical flask under the same conditions.
ResultThe highest embryogenic callus proliferation rate, 216.18% was obtained using 10 g embryogenic callus, 0.2 mg/L 2,4-D and 20% old culture medium inoculated in 100 mL liquid medium in the ALB.
ConclusionCompared with conical flask suspension culture, the growth rate of embryogenic callus in ALB system was 2.15 times faster in a week. Microscopic observation shows that the proliferated embryogenic callus is stable and high quality. This study provides technical support for large-scale propagation of P. tabuliformis based on somatic embryo system.
- Pinus tabuliformis,
- bioreactor,
- embryogenic callus

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