Composition and amounts of fatty acids in Perilla frutescens seed oils of different varieties and areas.
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Abstract
We applied soxhlet extraction to obtain seed oil of 17 kinds of germplasm from 11 different areas of China, belonging to five varieties of the genus Perilla and then used GC-MS to analyze the amounts of oil as well as the composition and relative amounts of fatty acids. Fourteen fatty acids were indentified from P. frutescens seed oil. These were divided into two groups of six high level (0.8%) and eight low level (≤ 0.2%) amounts of fatty acids. For the first time 14-methyl-hexadecanoic acid (C17∶0), 18 carbon diene acid (C18∶2n3) and 23 carbon acid (C23∶0) were reported in P. frutescens oil. The relative amount of α-linolenic acid was the highest with a range of 51.92% to 62.98%. Certain metabolic conversion relationships were detected among α-linolenic, linoleic and oleic acids. The results calculated with an external standard method for the amount of fatty acids in P. frutescens seeds implies that P. frutescens var. frutescens, among the five varieties investigated, is preferable as the raw material for P. frutescens oil, given its high rate of oil rate and its amount of α-linolenic acid.
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