Analysis of differentially expressed transcripts in early and middle development of Betula platyphylla female inflorescence

ZHAO Li-hong; ZHOU Fei; GUAN Min-xiao; LIU Chuang; ZHAO Xin; LIU Xue-mei

doi:DOI:10.13332/j.1000-1522.20140301

Journal of Beijing Forestry University > 2015 > 37(4): 104-112. > DOI: DOI:10.13332/j.1000-1522.20140301

ZHAO Li-hong, ZHOU Fei, GUAN Min-xiao, LIU Chuang, ZHAO Xin, LIU Xue-mei. Analysis of differentially expressed transcripts in early and middle development of Betula platyphylla female inflorescence[J]. Journal of Beijing Forestry University, 2015, 37(4): 104-112. DOI: DOI:10.13332/j.1000-1522.20140301

Citation:

PDF (5847 KB)

Analysis of differentially expressed transcripts in early and middle development of Betula platyphylla female inflorescence

College of Life Science, Northeast Forestry University, Harbin, Heilongjiang, 150040, P.R.China

More Information

Received Date: September 03, 2014
Revised Date: December 08, 2014
Published Date: April 29, 2015

Graphical Abstract

Abstract

Abstract

Betula platyphylla is a monoecious tree species with unisexual flowers, which plays an important role in the study of floral development of woody plants. This study aims to initially identify gene expression and regulation during the critical periods of female flower development in B. platyphylla, megasporogenesis (prophase) and gametophyte development (metaphase), and to discover genes related to female floral development. Firstly, we ascertained the prophase and metaphase in female floral development by the cytological method. Secondly, the establishment of forward and reverse suppression subtractive hybridization (SSH) cDNA libraries BHHQ and BHHZ were carried out with the cDNA of prophase and metaphase female inflorescence as tester and driver respectively, using SSH technique. A total of 1406 high quality EST sequences were obtained, 787 of them having high homologies with the nucleic acid or protein sequences in databases and 47 genes possibly associating with flower development. The results of GO analysis showed that 34.96% of these genes are mainly involved in biosynthesis process, 16.45% in cellular component and 48.59% in molecular function. Six flower development-related genes were analyzed by qRT-PCR analysis and the results showed that each of these genes has some expression in different tissues. Among them, the expression level of bhhq.408,bhhq.435,bhhz.359, and bhhz.215 in reproductive organs was significantly higher than that in other organs, respectively; both bhhq.254 and bhhq.190 have some expression in most tissues, confirming that these genes are not only involved in the development of reproductive organs, but also play some roles in vegetative organs.
- Suppression Subtractive Hybridization (SSH),
- floral development,
- Betula platyphylla,
- unisexual flower,
- transcriptional expression

FullText(HTML)

References (40)

References

[1]	包仁艳,姜春宁,郑彩霞,等.植物雌配子体发育的分子调控研究进展[J]. 北京林业大学学报,2005,27(4):90-96.
[1]	BAO R Y, JIANG C N,ZHENG C X, et al. Molecular mechanism of the regulation of female gametophyte development in plants[J].Journal of Beijing Forestry University,2005,27(4):90-96.
[2]	WANG C J,NAN G L,KELLIHER T, et al. Maize multiple archesporial cells 1(mac1),or ortholog of rice TDL1A,modulates anther development[J].Development,2012,139:2594-2603.
[2]	LIU X M, LIU Y, SONG F N, et al.Differential expression analysis of transcripts at early developmental stage of male inflorescence mutant of white birch(Betula platyphylla)by cDNA-AFLP[J]. Scientia Silvae Sinvce,2012,48(5):20-28.
[3]	GUAN M X,LIU X M,ZHANG Y,et al. Isolation and transcription expression analysis of SPL8 transcription factors gene of Betula platyphylla [J]. Journal of Nanjing Forestry University: Natural Sciences Edition,2013,37(3):17-22.
[3]	VENKATESAN S, MONICA A S. Pattern formation in miniature:the female gametophyte of fiowering plants[J]. Development,2010,137:179-189.
[4]	HOWDEN R, PARK S K, MOORE J M, et al. Selection of T-DNA-tagged male and female gametophytic mutants by segregation distortion in Arabidopsis[J]. Genetics,1998,149(2):621-631.
[4]	LIU F F,LI H Y,WANG S, et al. Cloning and periodic expression of BpSOC1 gene in Betula platyphylla[J]. Journal of Northeast Forestry University,2011,39(4):1-4.
[5]	WANG C,YANG C P,WEI J C, et al.Construction and EST analysis of suppression subtractive hybridization library of Betula platyphylla female inflorescence [J].Bulletin of Botanical Research,2008,28(3):293-298.
[5]	HUANG B Q, SHERIDAN W F. Embryo sac development in the maize indeterminate gametophyte 1 mutant: abnormal nuclear behavior and defective microtubule organization[J]. Plant Cell,1996,8(8):1391-1407.
[6]	刘雪梅,刘瀛,宋福南,等.白桦雄花突变体早期发育差异基因的cDNA-AFLP分析[J]. 林业科学,2012,48(5):20-28.
[6]	GUO S, SHEN H L,YANG W C,et al. Isolation of fertility restoration-related ESTs in pepper cytoplasmic male sterility lines using SSH[J]. Acta Horticulturae Sinica,2009,36(10):1443-1449.
[7]	CHEN C,WANG G. A method for intact stain by Ehrlich haematoxylin in the semithin sections[J]. Chin Bull Bot,1992,9(4): 53-54.
[7]	官民晓,刘雪梅,张妍,等.白桦SPL8转录因子基因的分离及转录表达分析[J]. 南京林业大学学报:自然科学版,2013,37(3):17-22.
[8]	KANG H Q,CHANG H Y,XU Y B, et al.Improvement of paraffin section methods and structural observation to endosperm development of rice kernel[J]. Acta Bot Boreal-Occident Sin,2008,28(5):1069-1074.
[8]	LEMMETYINEN J, HASSINEN M, ELO A, et al. Functional characterization of SEPALLATA3 and AGAM OUS orthologues in silver birch[J]. Physiologia Plantarum,2004,121(1):149-162.
[9]	ZENG F S,NAN N,ZHAN Y G. Extraction of total RNA from mature leaves rich in polysaccharides and secondary metabolites of Betula platyphylla Suk.[J]. Plant Physiology Communications,2007,43(5):913-916.
[9]	LANNENPAA M, JANONEN I, HOLTTA-VNORI M, et al. A new SBP box gene SPLI in silver birch (Betula pendula) [J]. Physiologia Plantarum,2004,120(3):491-500.
[10]	JIA X M, ZHANG H L. FT gene with a CaMV35S promoter to control early flowering of transgenic poplar[J]. Journal of Zhejiang A ＆ F University,2014,31(3):404-409.
[10]	ELO A, LEMMETYINEN J, TURUNEN M, et al. Three MADS-box genes similar to APETALA1 and FRUITFULL from silver birch(Betula pendula) [J]. Physiologia Plantarum,2001,112(1):95-103.
[11]	MA L L, QIU L L, FU Y W, et al.Isolation and expression of Flowering Locus T(FT) gene in Betula platyphylla[J]. Bulletin of Botanical Research,2012,32(6):737-743.
[11]	刘菲菲,李慧玉,王姗,等.白桦BpSOCI基因的克隆及时序表达分析[J].东北林业大学学报,2011,39(4):1-4.
[12]	ZHANG Y,LIU Y,SUN F B, et al.Isolation and expression of APETALA2 transcription factor gene in Betula platyphylla[J]. Forest Research,2012,25(2):254-260.
[12]	王超,杨传平,魏继承,等.白桦雌花序抑制性消减文库构建及EST分析[J].植物研究,2008,28(3):293-298.
[13]	郭爽,沈火林,杨文才,等.利用抑制消减杂交技术分离辣椒细胞质雄性不育育性恢复相关EST[J].园艺学报,2009,36(10):1443-1449.
[14]	陈超,王桂兰.爱氏苏木精整体染色法在树脂薄切片中的应用[J].植物学报,1992,9(4):53-54.
[15]	康海岐,常红叶,许育彬,等.水稻籽粒胚乳的石蜡切片方法改良及其结构发育观察[J].西北植物学报,2008,28(5):1069-1074.
[16]	曾凡锁,南楠,詹亚光. 富含多糖和次生代谢产物的白桦成熟叶中总RNA的提取[J].植物生理学通讯,2007,43(5):913-916.
[17]	YIN X R, ALLAN A C, CHEN K S, et al. Kiwifruit EIL and ERF genes involved in regulating fruit ripening[J]. Plant Physiol,2010,153(3):1280-1292.
[18]	ROBER-KLEBER N, ALBRECHTOVÁ J T, FLEIG S. Plasma membrane H+-ATPase is involved in auxin-mediated cell elongation during wheat embryo development[J]. Plant Physiol,2003,131(3):1302-1312.
[19]	PARENICOVÁ L, DE FOLTER S, KIEFFER M. Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: new openings to the MADS world[J]. Plant Cell,2003,15(7):1538-1551.
[20]	LI D, LIU C, SHEN L, et al. A repressor complex governs the integration of flowering signals in Arabidopsis[J]. Dev Cell,2008,15(1):110-120.
[21]	贾小明,张焕玲.CaMV35S 启动子驱动的 FT 基因调控杨树早期开花的初步研究[J].浙江农林大学学报,2014,31(3):404-409.
[22]	马丽丽,邱丽丽,付扬威,等.白桦开花位点Flowering Locus T(FT)基因的分离及其表达[J].植物研究,2012,32(6):737-743
[23]	COLASANTI J, YUAN Z, SUNDARESAN V. The indeterminate gene encodes a zinc finger protein and regulates a leaf-generated signal required for the transition to flowering in maize[J]. Cell,1998,93(4):593-603.
[24]	LIU S R, KHAN M R G, LI Y P. Comprehensive analysis of CCCH-type zinc finger gene family in citrus (Clementine mandarin) by genome-wide characterization[J]. Mol Genet Genomics,2014,289(5):855-872.
[25]	KENICHI K, YOSHIAKI K, TOKUZO N, et al. Zinc-finger genes that specifically express in pistil secretory tissues of petunia[J]. Plant Cell Physiol,2000,41(3):377-382.
[26]	LUO M, BILODEAU P, KOLTUNOW A,et al.Genes controlling fertilization-independent seed development in Arabidopsis thaliana[J]. Pro Natl Acad Sci USA,1999,96(1):296-301.
[27]	DU H W, HUANG M, ZHANG Z X. Genome-wide analysis of the AP2/ERF gene family in maize waterlogging stress response[J]. Euphytica,2014,198(1):115-126.
[28]	张妍,刘瀛,孙丰滨,等.白桦APETALA2(AP2)转录因子基因的分离及其表达[J].林业科学研究,2012,25(2):254-260.

Cited By

Get Citation

PDF

XML

Article views (1811) PDF downloads (31)

Turn off MathJax

Article Contents

Abstract

References

Analysis of differentially expressed transcripts in early and middle development of Betula platyphylla female inflorescence

Abstract

References

Catalog

Export File

Citation

Format

Content