Advances in research of pollen cryopreservation

In order to provide conservation and exchange of germplasm resources, and satisfy the requirement of hybridization, pollen cryopreservation has been well developed. Crops, fruits, medicinal and ornamental plants and other material have been involved. Many studies concentrated on the techniques of pollen cryopreservation. With the development of the technique, mechanisms of pollen cryopreservation were initiated. Effects of post-LN pollen were tested not only by staining and germination methods, but also through hybridization. This paper reviewed the plants that had been cryopreserved successfully during the last twenty years, mainly introduced the techniques of pollen cryopreservation and evaluation methods of thawed pollen. Meanwhile, the mechanism research process of pollen cryopreservation was reviewed.