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YAO Hong-jun, SHI Yu-jie, XU Bing-jiu, GAO Rong-fu. Isolation of lipids from photosystem Ⅰ complex in Pisum sativum L.and its characterization with RP-HPLC[J]. Journal of Beijing Forestry University, 2006, 28(5): 95-98.
Citation: YAO Hong-jun, SHI Yu-jie, XU Bing-jiu, GAO Rong-fu. Isolation of lipids from photosystem Ⅰ complex in Pisum sativum L.and its characterization with RP-HPLC[J]. Journal of Beijing Forestry University, 2006, 28(5): 95-98.
shu

Isolation of lipids from photosystem Ⅰ complex in Pisum sativum L.and its characterization with RP-HPLC

  • 1. Key Laboratory for Genetic and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, 100083, P. R. China;

  • 2. School of Pharmaceutical Sciences, Peking University, Beijing, 100083, P. R. China

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  • Received Date: May 21, 2005
  • Available Online: May 14, 2024
    Graphical Abstract
    • Abstract
  • In order to discuss the distribution and function of lipids in thylakoid membranes and its protein complex,lipids were extracted from photosystem I separated by DOC-PAGE from thylakoid membranes in the leaves of Pisum sativum L.An RP-HPLC-UV method with gradient elution was established for the separation of lipids obtained from thylakoid membranes,and baseline separation of the sample components could be realized in 25 min with this method.From the UV spectrum extracted from the DAD 3D graph,the main components separated in HPLC can be characterized preliminarily as lipids.
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