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JIA Xiang-nan, LI Wei, SHEN Jun-ling, OUYANG Kun-xi, CHEN Xiao-yang. Construction of multi-gene expression vector and tobacco transformation based on Cre-LoxP recombination system[J]. Journal of Beijing Forestry University, 2010, 32(5): 121-125.
Citation: JIA Xiang-nan, LI Wei, SHEN Jun-ling, OUYANG Kun-xi, CHEN Xiao-yang. Construction of multi-gene expression vector and tobacco transformation based on Cre-LoxP recombination system[J]. Journal of Beijing Forestry University, 2010, 32(5): 121-125.
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Construction of multi-gene expression vector and tobacco transformation based on Cre-LoxP recombination system

  • 1 National Engineering Laboratory of Forest Tree Breeding，Beijing Forestry University，100083，P.R.China; 2 College of Forestry, South China Agricultural University, Guangzhou，510642，P.R.China.

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  • Received Date: December 31, 1899
  • Revised Date: December 31, 1899
  • Published Date: September 29, 2010
    Graphical Abstract
    • Abstract
  • Using a system of multi-gene plant expression vector based on a set of Cre-LoxP recombination system, the BtCryIAc, BADH, pttGA20ox and rolB genes were inserted into the plant expression vector pYL1305, which was named pYL1305BBGR. The tabacco was transformed by leaf disc method mediated by Agrobacterium tumefaciens. Tested by PCR and Southern blot, the multi genes have been successfully transferred into the genome of tobacco. The semi-quantitative RT-PCR analysis showed that all the 4 genes were expressed normally.
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