Transformation of ground-cover Chrysanthemum with HsfA2 gene isolated from Arabidopsis thaliana

A HsfA2 gene isolated from Arabidopsis thaliana ecotype Columbia was transferred into ground-cover Chrysanthemum ‘Beilinhuang’ by the Agrobacterium-mediated method. After antibiotics screening and molecular detection, eight positive transgenic lines were obtained. RT-PCR detection results showed that the exogenous AtHsfA2 gene was expressed in seven transgenic lines. The results of heat stress treatment also showed that the ability of transgenic plants to heat stress was significantly higher than that of non-transgenic plants. This research obtained Chrysanthemum lines with higher resistance ability to heat stress through transgenic method and may provide scientific references for transgenic breeding of ornamental plants.