Exploring the original causes of cherry cultivar Manaohong originated from Nayong County, Guizhou Province using ISSR markers.

In this paper, genetic relationship among local cherry cultivar Manaohong originated from Nayong County of Guizhou Province, as well as: sour cherry, black pearl and four sweet cherry cultivars were investigated using ISSR markers. The purpose was to investigate the causes of variation on Manaohong. A total of 286 markers were scored from 15 primers, which were demonstrated highly reproducible, clear and polymorphic bands. Among the obtained markers, 249 were polymorphic, accounting for 87.06% of the total. As analyzed by NTSYS2.01, the genetic similarity among the seven accessions ranged from 0.41 to 0.94, and the genetic similarity between Manaohong and sour cherry was 0.57Manaohong has significant genetic differences with sour cherry on molecular level, possibly it is a hybridization variation of sour cherry.