Ipt gene promoting shoot regeneration in genetic transformation of Eucommia ulmoides Oliv

In this research，the ipt gene driven by 35S promoter was transferred into Eucommia ulmoides Oliv. via agrobacteriummediated transformation, and the influence of exogenous gene on shoot regeneration was further investigated in the current work. The results showed that compared with control (iptfree) line (0.93%), no significant difference in shoot regeneration efficiency was obtained from the ipt transgenic lines (0.96%). However, ipt gene enhanced the shoot number per explant, which was 31, obviously higher than that of control (1.9). Thus ipt gene enhanced shoot inductivity of E. ulmoides , which was 2.95%, evidently high than CK 1.68%. The present research firstly prove that ipt gene can substantially enhance shoot regeneration of E. ulmoides and this lays a foundations for promoting genetic transformation efficiency of E. ulmoides.