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SHI Jun-na, LIU Mei-qin, LIU Jie, CHEN Yu-zhen, LU Cun-fu. Sequence analysis and expression pattern of AmSTZF encoding an A20/AN1 zinc finger protein in Ammopiptanthus mongolicus.[J]. Journal of Beijing Forestry University, 2012, 34(2): 103-108.
Citation: SHI Jun-na, LIU Mei-qin, LIU Jie, CHEN Yu-zhen, LU Cun-fu. Sequence analysis and expression pattern of AmSTZF encoding an A20/AN1 zinc finger protein in Ammopiptanthus mongolicus.[J]. Journal of Beijing Forestry University, 2012, 34(2): 103-108.
shu

Sequence analysis and expression pattern of AmSTZF encoding an A20/AN1 zinc finger protein in Ammopiptanthus mongolicus.

  • National Engineering Laboratory for Tree Breeding，Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education，Beijing Forestry University，100083，P.R.China.

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  • Received Date: December 31, 1899
  • Revised Date: December 31, 1899
  • Published Date: March 29, 2012
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    • Abstract
  • A20/AN1 zinc finger protein widely exists in eukaryote and plays a central role in signal transduction pathway involved in stress response.We cloned zinc finger gene AmSTZF from cold and droughttreated Ammopiptanthus mongolicus leaves by bioinformatics and RTPCR. The cDNA of AmSTZF encodes a polypeptide with 172 amino acids,which contains A20 and AN1 conserved domains and belongs to A20/AN1 zinc finger protein.Bioinformatics predicts that AmSTZF has phosphorylation sites, exists in cytoplasm and is involved in the regulation of gene transcription.RTPCR analysis shows that the expression of AmSTZF is upregulated under cold-and drought-stresses and that AmSTZF is related to the response to drought and chilling stresses.
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