Sequence analysis and expression pattern of AmSTZF encoding an A20/AN1 zinc finger protein in Ammopiptanthus mongolicus.

A20/AN1 zinc finger protein widely exists in eukaryote and plays a central role in signal transduction pathway involved in stress response.We cloned zinc finger gene AmSTZF from cold and droughttreated Ammopiptanthus mongolicus leaves by bioinformatics and RTPCR. The cDNA of AmSTZF encodes a polypeptide with 172 amino acids,which contains A20 and AN1 conserved domains and belongs to A20/AN1 zinc finger protein.Bioinformatics predicts that AmSTZF has phosphorylation sites, exists in cytoplasm and is involved in the regulation of gene transcription.RTPCR analysis shows that the expression of AmSTZF is upregulated under cold-and drought-stresses and that AmSTZF is related to the response to drought and chilling stresses.