Objective As a newly developed woody oil tree species, Idesia polycarpa has broad development prospects. Idesia polycarpa “Exuan 1”, as a newly identified new variety, has the characteristics of high and stable yield, strong adaptability and so on. The demand for high-quality seedlings of Idesia polycarpa “Exuan 1” is increasing, so it is urgent to build an efficient breeding system to meet the wide application of Idesia polycarpa “Exuan 1” in the industry.
Method Based on the optimization of explant disinfection method, hormone combination of adventitious bud induction medium, hormone combination of adventitious bud proliferation medium and hormone combination of tissue culture seedling rooting medium, tissue culture technology was used to construct a tissue culture and breeding system of Idesia polycarpa “Exuan 1”. In this study, we systematically studied the disinfection methods of the explants of Idesia polycarpa “Exuan 1” and the effects of plant hormones on the process of bud induction, proliferation and rooting.
Result (1) Using 75% ethanol treatment for 30 s combined with 0.1% HgCl2 treatment for 8 min was the best disinfection method for the explants of Idesia polycarpa “Exuan 1”. (2) 1/2MS + 0.03 mg/L TDZ + 1.5 mg/L 6-BA + 0.05 mg/L NAA medium combination was suitable for adventitious bud induction of Idesia polycarpa “Exuan 1”. (3) The combination of 1/2MS + 0.2 mg/L TDZ + 2.0 mg/L 6-BA + 0.05 mg/L NAA medium was suitable for adventitious bud proliferation of tissue culture of Idesia polycarpa “Exuan 1”. (4) The combination of 1/2MS + 0.3 mg/L NAA + 0.5 mg/L IAA medium was suitable for rooting induction of tissue culture seedlings of Idesia polycarpa “Exuan 1”. Under this condition, the rooting rate of tissue culture seedlings reached 98%.
Conclusion In this study, we construct a rapid propagation system of Idesia polycarpa “Exuan 1”, which lays a foundation for the popularization and application as well as further research on Idesia polycarpa “Exuan 1” at the molecular level.
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