Construction of tissue culture breeding system of <i>Idesia polycarpa </i> “Exuan 1”

Chen Yaobing; Luo Kai; Li Meidong; Huang Xiufang; Liu Hanzhen; Wang Shuiqing; Chen Shenglin

doi:10.12171/j.1000-1522.20210116

Journal of Beijing Forestry University > 2022 > 44(12): 23-31. > DOI: 10.12171/j.1000-1522.20210116

Chen Yaobing, Luo Kai, Li Meidong, Huang Xiufang, Liu Hanzhen, Wang Shuiqing, Chen Shenglin. Construction of tissue culture breeding system of Idesia polycarpa “Exuan 1”[J]. Journal of Beijing Forestry University, 2022, 44(12): 23-31. DOI: 10.12171/j.1000-1522.20210116

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Construction of tissue culture breeding system of Idesia polycarpa “Exuan 1”

1.
Hubei Engineering Research Center of Selenium Food Nutrition and Health Intelligent Technology, Hubei Minzu University, Enshi 445000, Hubei, China
2.
Hubei Xuzhou Forestry and Agriculture Technology Co., Ltd., Lichuan 445400, Hubei, China
3.
Chinese Forestry Industry Association, Beijing 100714, China

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Received Date: May 05, 2021
Revised Date: July 05, 2021
Accepted Date: October 09, 2022
Available Online: October 10, 2022
Published Date: December 24, 2023

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Abstract

Abstract

Objective As a newly developed woody oil tree species, Idesia polycarpa has broad development prospects. Idesia polycarpa “Exuan 1”, as a newly identified new variety, has the characteristics of high and stable yield, strong adaptability and so on. The demand for high-quality seedlings of Idesia polycarpa “Exuan 1” is increasing, so it is urgent to build an efficient breeding system to meet the wide application of Idesia polycarpa “Exuan 1” in the industry.
Method Based on the optimization of explant disinfection method, hormone combination of adventitious bud induction medium, hormone combination of adventitious bud proliferation medium and hormone combination of tissue culture seedling rooting medium, tissue culture technology was used to construct a tissue culture and breeding system of Idesia polycarpa “Exuan 1”. In this study, we systematically studied the disinfection methods of the explants of Idesia polycarpa “Exuan 1” and the effects of plant hormones on the process of bud induction, proliferation and rooting.
Result (1) Using 75% ethanol treatment for 30 s combined with 0.1% HgCl₂ treatment for 8 min was the best disinfection method for the explants of Idesia polycarpa “Exuan 1”. (2) 1/2MS + 0.03 mg/L TDZ + 1.5 mg/L 6-BA + 0.05 mg/L NAA medium combination was suitable for adventitious bud induction of Idesia polycarpa “Exuan 1”. (3) The combination of 1/2MS + 0.2 mg/L TDZ + 2.0 mg/L 6-BA + 0.05 mg/L NAA medium was suitable for adventitious bud proliferation of tissue culture of Idesia polycarpa “Exuan 1”. (4) The combination of 1/2MS + 0.3 mg/L NAA + 0.5 mg/L IAA medium was suitable for rooting induction of tissue culture seedlings of Idesia polycarpa “Exuan 1”. Under this condition, the rooting rate of tissue culture seedlings reached 98%.
Conclusion In this study, we construct a rapid propagation system of Idesia polycarpa “Exuan 1”, which lays a foundation for the popularization and application as well as further research on Idesia polycarpa “Exuan 1” at the molecular level.
- Idesia polycarpa “Exuan 1”,
- tissue culture,
- breeding system

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References

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