- Scopus
- Chinese Science Citation Database (CSCD)
- A Guide to the Core Journal of China
- CSTPCD
- F5000 Frontrunner
- RCCSE
| Citation: |
Du Kang, Wang Jiahuan, Li Junheng, Li Chao, Zhang Pingdong, Kang Xiangyang. Genetic identification and evaluation of cold-resistant germplasm resources in Populus tomentosa[J]. Journal of Beijing Forestry University, 2023, 45(12): 59-67. DOI: 10.12171/j.1000-1522.20220363
|
This paper identifies the resources of Populus tomentosa in Shuozhou City, Shanxi Province of northern China, compares and analyzes the genetic structure and genetic diversity differences of the populations of poplar in Guanxian County, Shandong Province of eastern China, and evaluates the cold resistance of the existing poplar in Shuozhou City.
Using 370 individuals in Shuozhou City and 441 clones in Guanxian County, SSR molecular markers were used to carry out the identification of poplar resources, population genetic structure and genetic diversity analysis, and to evaluate the growth index and low temperature tolerance.
Using 13 pairs of primers, 370 individuals of P. tomentosa were divided into 183 clones. A total of 67 allelic variations were detected, with an average of 5 allelic variations per locus, and the polymorphism information content ranged from 0.306 to 0.735. Compared with the Guanxian County Germplasm Resource Bank in Shandong Province, it was found that the same clone resources were not preserved during the ex-situ preservation of P. tomentosa. The difference in genetic diversity was not significant. The 624 clones preserved in the two sites can be divided into seven clusters. The 183 extant clones in Shuozhou City were classified into five classes by cold tolerance evaluation, including 47 cold hardy clones in classⅠ, and the cold hardy poplars also had higher tree height and wood volume growth.
Natural selection has caused the north and south germplasm repository of P. tomentosa to eliminate part of the germplasm that deviates from the environment of the preservation site, and to retain the germplasm resources that are suitable for the environment of the preservation site. The genetic structure of two populations differs considerably, indicating the need to collect, conserve and use forest germplasm resources in different regions. The existing female asexual lines in the germplasm repository of Shuozhou City, Shanxi Province, are valuable cold-tolerant breeding resources, which are of great value for the construction of a suitable population of northern poplar and the genetic improvement of high rotation.
| [1] |
朱之悌. 毛白杨遗传改良 [M]. 北京: 中国林业出版社, 2006.
Zhu Z T. Genetic improvement of Populus tomentosa Carr. [M]. Beijing: China Forestry Publishing House, 2006.
|
| [2] |
朱之悌. 全国毛白杨优树资源收集、保存和利用的研究[J]. 北京林业大学学报, 1992, 14(增刊3): 1−25.
Zhu Z T. Collection, conservation and utilization of plus tree resources of Populus tomentosa in China[J]. Journal of Beijing Forestry University, 1992, 14(Suppl. 3): 1−25.
|
| [3] |
朱之悌, 康向阳, 张志毅. 毛白杨天然三倍体选种研究[J]. 林业科学, 1998, 34(4): 22−31.
Zhu Z T, Kang X Y, Zhang Z Y. Studies on selection of natural triploids of Populus tomentosa[J]. Scientia Silvae Sinicae, 1998, 34(4): 22−31.
|
| [4] |
赵勇刚, 高克姝. 毛白杨种源试验研究[J]. 山西林业科技, 1994(4): 19−24.
Zhao Y G, Gao K S. Experimental study on provenance of Populus tomentosa[J]. Shanxi Forestry Science and Technology, 1994(4): 19−24.
|
| [5] |
高克姝, 温高明. 毛白杨抗寒无性系选育[J]. 山西林业科技, 1998(4): 5−9.
Gao K S, Wen G M. Breeding of cold-resistant clones of Populus tomentosa[J]. Shanxi Forestry Science and Technology, 1998(4): 5−9.
|
| [6] |
康向阳. 新一轮毛白杨遗传改良策略的思考和实践[J]. 北京林业大学学报, 2016, 38(7): 1−8.
Kang X Y. Thinking and practices for strategy on a new round genetic improvement of Populus tomentosa[J]. Journal of Beijing Forestry University, 2016, 38(7): 1−8.
|
| [7] |
康向阳. 关于林木无性系育种策略的思考[J]. 北京林业大学学报, 2019, 41(7): 1−9.
Kang X Y. Thinking on breeding strategy of forest clones[J]. Journal of Beijing Forestry University, 2019, 41(7): 1−9.
|
| [8] |
Guichoux E, Lagache L, Wagner S, et al. Current trends in microsatellite genotyping[J]. Molecular Ecology Resources, 2011(4): 11.
|
| [9] |
Dayanandan S, Rajora O P, Bawa K S. Isolation and characterization of microsatellites in trembling aspen ( Populus tremuloides)[J]. Theoretical & Applied Genetics, 1998, 96(6−7): 950−956.
|
| [10] |
Yin T M, Difazio S P, Gunter L E, et al. Large-scale heterospecific segregation distortion in Populus revealed by a dense genetic map[J]. Theoretical and Applied Genetics, 2004, 109(3): 451−463. doi: 10.1007/s00122-004-1653-5
|
| [11] |
Liu H, Yang W, Hou J, et al. Genetic identification of 43 elite clonal accessions of Populus deltoides by SSR fingerprinting[J]. Canadian Journal of Plant Science, 2016, 96(3): 494−502.
|
| [12] |
Hadian J, Kavand S, Ahmadi J, et al. Genotypic diversity and structure of Satureja mutica revealed by inter-simple sequence repeat markers[J]. Biochemical Systematics & Ecology, 2014, 54: 48−52.
|
| [13] |
Du Q Z, Gong C R, Pan W, et al. Development and application of microsatellites in candidate genes related to wood properties in the Chinese white poplar ( Populus tomentosa Carr.)[J]. DNA Research, 2013, 20(1): 31−44. doi: 10.1093/dnares/dss031
|
| [14] |
Bai F Y, Kang N, Zhang P D, et al. Selection of female parents with high fertility and high combining abilities for cross-breeding Populus tomentosa[J]. Journal of Forestry Research, 2019, 30(2): 69−74.
|
| [15] |
韩志强, 任勇谕, 夏宇飞, 等. 毛白杨多态SSR引物库和种质资源指纹图谱库构建[J]. 北京林业大学学报, 2019, 41(7): 10−18.
Han Z Q, Ren Y Y, Xia Y F, et al. Construction of polymorphic SSR primer library and germplasm resource fingerprint database of Populus tomentosa[J]. Journal of Beijing Forestry University, 2019, 41(7): 10−18.
|
| [16] |
Markus S. An economic method for the fluorescent labeling of PCR fragments[J]. Nature Biotechnology, 2000, 18: 233−234. doi: 10.1038/72708
|
| [17] |
Hulce D, Li X, Snyderleiby T, et al. Gene marker genotyping software: tools to increase the statistical power of DNA fragment analysis[J]. Journal of Biomolecular Techniques, 2011, 22(Suppl.): S35.
|
| [18] |
Slate J, Marshall T, Pemberton J. A retrospective assessment of the accuracy of the paternity inference program CERVUS[J]. Molecular Ecology, 2010, 9(6): 801−808.
|
| [19] |
Liu K, Muse S V. Power marker: an integrated analysis environment for genetic marker analysis[J]. Bioinformatics, 2005, 21(9): 2128−2129. doi: 10.1093/bioinformatics/bti282
|
| [20] |
Nei M, Tajima F, Tateno Y. Accuracy of estimated phylogenetic trees from molecular data[J]. Journal of Molecular Evolution, 1983, 19(2): 153−170. doi: 10.1007/BF02300753
|
| [21] |
Pritchard J K, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data[J]. Genetics, 2000, 155(4): 9197−9201.
|
| [22] |
聂兴华, 刘松, 王碧瑶, 等. 基于SSR标记的我国主栽日本栗品种(系)遗传结构分析和指纹图谱构建[J]. 核农学报, 2022, 36(11): 2104−2114.
Nie X H, Liu S, Wang B Y, et al. Genetic structure analysis and fingerprinting construction of the main Japanese chestnut cultivars (lines) using SSR markers in China[J]. Acta Agriculturae Nucleatae Sinica, 2022, 36(11): 2104−2114.
|
| [23] |
陈章水. 杨树二元立木材积表的编制[J]. 林业科学研究, 1989, 2(1): 78−83.
Chen Z S. The establishment of binomial stock volume tables of poplar[J]. Forest Research, 1989, 2(1): 78−83.
|
| [24] |
宋跃朋. 毛白杨花发育遗传调控研究[D]. 北京: 北京林业大学, 2013.
Song Y P. Genetic regulation of floral development in Populus tomentosa[D]. Beijing: Beijing Forestry University, 2013.
|
| [25] |
黄智慧. 毛白杨无性系地理变异的研究[J]. 北京林业大学学报, 1992(增刊3): 33−42.
Huang Z H. Study on geographic variation of Populus tomentosa clones[J]. Journal of Beijing Forestry University, 1992(Suppl. 3): 33−42.
|
| [26] |
何承忠. 毛白杨遗传多样性及起源研究[D]. 北京: 北京林业大学, 2005.
He C Z. Study on genetic diversity and origin of Populus tomentosa[D]. Beijing: Beijing Forestry University, 2005.
|
| [27] |
王博文, 王保垒, 张曼, 等. 毛白杨木材形成功能基因内SSR标记的开发及评价[J]. 林业科学, 2010, 46(11): 8−15.
Wang B W, Wang B L, Zhang M, et al. Development and evaluation of simple sequence repeat (SSR) loci from functional genes involved in wood formation in Populus tomentosa[J]. Scientia Silvae Sinicae, 2010, 46(11): 8−15.
|
| [28] |
白凤莹. 毛白杨优树资源评价与育种亲本群体构建[D]. 北京: 北京林业大学, 2015.
Bai F Y. Evalution of elite tree resource and construction of parent population for breeding program me in Populus tomentosa Carr.[D]. Beijing: Beijing Forestry University, 2015.
|
| [29] |
朱大保. 毛白杨有性生殖能力的研究[J]. 北京林业大学学报, 1990, 12(1): 1−9. doi: 10.13332/j.1000-1522.1990.01.001
Zhu D B. Rsearch on the reproduction capacity of Populus tomentosa[J]. Journal of Beijing Forestry University, 1990, 12(1): 1−9. doi: 10.13332/j.1000-1522.1990.01.001
|
| [30] |
段安安, 杨敏生, 朱之悌. 毛新 × 银灰双杂种无性系抗寒抗旱性的生理测定[J]. 云南林业科技, 1998(3): 3−5.
Duan A A, Yang M S, Zhu Z T. Physiological measurement of cold and drought resistance of clones ( Populus tomentosa × P. bolleana) × P. canescens[J]. Yunnan Forestry Science and Technology, 1998(3): 3−5.
|
| [1] | Zhou Kerou, Chen Zhuo, Yu Zhucheng, Zhong Yang, Shang Ce. Population structure and genetic diversity of Bretschneidera sinensis in Xianxialing Nature Reserve, Zhejiang Province of eastern China[J]. Journal of Beijing Forestry University, 2024, 46(11): 76-82. DOI: 10.12171/j.1000-1522.20230211 |
| [2] | Guo Xiaobing, Huang Zhixin, Zhang Tianxiang, Ding Jianhong, Gao Yunyun, Fu Yajun, Hu Defu, Liu Gang. Analysis of mtDNA genetic diversity and genetic structure of captive forest musk deer population[J]. Journal of Beijing Forestry University, 2022, 44(3): 98-105. DOI: 10.12171/j.1000-1522.20210050 |
| [3] | Zhao Pan, Li Danyang, Ma Jinlin, Liang Wenhui, Pang Xiaoming, Long Cui, Ma Jingyi, Guo Huihong. SSR marker development, genetic diversity and population structure analysis in oil tree species Vernicia montana[J]. Journal of Beijing Forestry University, 2021, 43(11): 50-61. DOI: 10.12171/j.1000-1522.20210054 |
| [4] | Qu Kai, Guo Haoping, Wang Baorui, Zhou Wenling, Hou Lili, Li Qin, Li Jihong, Cheng Tiantian. Genetic diversity analysis of Chionanthus retusus natural population based on SRAP molecular markers[J]. Journal of Beijing Forestry University, 2020, 42(12): 40-50. DOI: 10.12171/j.1000-1522.20200212 |
| [5] | Yao Junxiu, Mao Xiuhong, Li Shanwen, Liu Xueliang, Wu Dejun. Genetic diversity of germplasm resources of Leuce based on SSR fluorescent marker[J]. Journal of Beijing Forestry University, 2018, 40(6): 92-100. DOI: 10.13332/j.1000-1522.20170429 |
| [6] | LI Yi-liang, ZHAO Fen-cheng, HU Yan-fei, CAI Jian, WU Hui-shan, ZHANG Ying-zhong, GUO Wen-bing. Analysis of genetic diversity from different geographical populations in Khaya senegalensis[J]. Journal of Beijing Forestry University, 2014, 36(5): 82-86. DOI: 10.13332/j.cnki.jbfu.2014.05.022 |
| [7] | LIAO Hui-rong, GU Wan-chun, MING Jun. Determining genetic diversity of natural population of Syringa oblatausing allozyme markers.[J]. Journal of Beijing Forestry University, 2009, 31(5): 84-89. |
| [8] | RUAN Zhi-ping, , XIANG Ping, LI Zhen-ji. Cold tolerance of Butia capitata，Acoelorrhaphe wrightii and Elaeis guineensis[J]. Journal of Beijing Forestry University, 2008, 30(4): 77-81. |
| [9] | ZHANG Yu-rong, LUO Ju-chun, YU Jin-xiu. Genetic diversity of the endangered plant Abies ziyuanensis detected by ISSR markers[J]. Journal of Beijing Forestry University, 2007, 29(6): 41-46. DOI: 10.13332/j.1000-1522.2007.06.012 |
| [10] | CHEN Wei. Genetic diversity of the natural populations of Betula luminifera[J]. Journal of Beijing Forestry University, 2006, 28(6): 28-34. |
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: