Soil microbial community structure and functional diversity in typical plantations marked by PLFA in hilly loess region

LIU Hai-yan; WEI Tian-xing; WANG Xian

doi:10.13332/j.1000--1522.20150262

Journal of Beijing Forestry University > 2016 > 38(1): 28-35. > DOI: 10.13332/j.1000--1522.20150262

LIU Hai-yan, WEI Tian-xing, WANG Xian. Soil microbial community structure and functional diversity in typical plantations marked by PLFA in hilly loess region[J]. Journal of Beijing Forestry University, 2016, 38(1): 28-35. DOI: 10.13332/j.1000--1522.20150262

Citation:

PDF (946 KB)

Soil microbial community structure and functional diversity in typical plantations marked by PLFA in hilly loess region

Key Laboratory of Soil and Water Conservation & Desertification Combating of Ministry of Education, Jixian Forest Ecosystem Observation and Experiment Station of China National Ecosystem Research Network,Beijing Forestry University,Beijing,100083,P.R. China.

More Information

Received Date: July 20, 2015
Revised Date: October 08, 2015
Published Date: January 30, 2016

Graphical Abstract

Abstract

Abstract

This study was aimed to discover the effects of different types of plantations on soil microbial community structure and functional diversity in hilly loess region. The study area is located in Jixian County, Shanxi Province of northern China. Two typical plantations (Robinia pseudoacacia and Pinus tabuliformis) were chosen and a grassland was set as control. Soil samples in the vertical layers (0-20 cm) were collected in August 2014, and soil microbial community structure was analyzed by phospholipid fatty acid (PLFA) analytical method. Results showed that the PLFA amounts of soil bacteria, fungi, actinomyces and Gram negative bacteria and Gram positive bacteria under the Robinia pseudoacacia plantation were all the highest in the three kinds of sample plots. Biomass of bacteria in three plots was significantly different, while that of fungi was roughly the same in the two plantations and significantly different with the grassland. Plantation improved the proportion of fungi. The phospholipid fatty acid structure of the two typical plantations was significantly different with the grassland. The correlation analysis showed that the amounts of the PLFAs were closely correlated to the soil fertility factors. Microbial community structure was correlated to plants: functional diversity was higher under the Robinia pseudoacacia plantation, followed by that under Pinus tabuliformis plantation, and grassland was the least. Therefore, for the microbial community, the two typical plantations can improve the microbial community structure and the soil quality as well, and Robinia pseudoacacia plantation was better.
- Robinia pseudoacacia,
- Pinus tabuliformis,
- plantation,
- microbial,
- phospholipid fatty acid(PLFA)

FullText(HTML)

References (48)

References

[1]	邢肖毅. 黄土丘陵区侵蚀环境对土壤氮素及微生物群落结构的影响[D]. 杨凌:西北农林科技大学,2013.
[1]	XING X Y. The effects of erosion environment on soil nitrogen and microbial community diversity on the hilly-gully area of Loess Plateau[D].Yangling: Northwest A F University,2013.
[2]	魏天兴, 朱金兆.黄土区人工林地水分供耗特点与林分生产力研究[J] .土壤侵蚀与水土保持学报, 1999, 5(4):45- 51.
[2]	WEI T X ,ZHU J Z. Feature of water supply and consumption and productivity of stand in loess region[J].Journal of Soil Erosion and Soil and Water Conservation,1999, 5(4):45- 51.
[3]	LI Y, XU X Q. Effective model of improving soil physical property by roots of Chinese pine plantation on the Loess Plateau[J].Scientia Silvae Sinicae,1993, 29(3):193- 198.
[3]	李勇, 徐晓琴.黄土高原油松人工林根系改善土壤物理性质的有效模式[J].林业科学,1993, 29(3):193- 198.
[4]	ZHANG S Q,WANG G D,SHI X L, et al. Improvement of soil hydro-physical properties by artificial Pinus tabulaeformis Carr. forests in the Loess Plateau[J].Agricultural Research in the Arid Areas,2005,23(1):60- 64.
[4]	张社奇, 王国栋, 时新玲,等.黄土高原油松人工林地土壤水分物理性质研究[J].干旱地区农业研究,2005,23(1):60- 64.
[5]	蔡晓布,钱成,彭岳林,等.西藏中部退化农田土壤肥力的变化特征及其重建[J].土壤学报,2004,41(4):603- 611.
[5]	CAI X B, QIAN C, PENG Y L, et al. Fertility and restoration of degraded soil in central Tibet[J]. Aeta Pedologiea, 2004,41(4): 603- 611.
[6]	SHEN C W, XIAO R L, XU H Q, et al. Effects of the cover and intercropping on soil microbial biomass of tea plantations in subtropical hilly region[J]. Journal of Soil and Water Conservation, 2006, 20(3): 141- 144.
[6]	沈程文, 肖润林,徐华勤,等.覆盖与间作对亚热带丘陵区茶园土壤微生物量的影响[J].水土保持学报,2006,20(3):141- 144.
[7]	WANG J W, FENG Y J. Effects of planting Bt corn on soil microbial activity and soil fertility[J]. Acta Ecolngica Sinica, 2005, 25(5): 1213- 1220.
[7]	王建武,冯远娇.种植Bt玉米对土壤微生物活性和肥力的影响[J].生态学报,2005,25(5):1213- 1220.
[8]	ZHANG S Q, WANG G D, TIAN P, et al. Distributive feature of soil microorganism of Robinia pseudoacacia L. plantation forestland in Loess Plateau[J]. Journal of Soil and Water Conservation, 2004,18(6):128- 131.
[8]	张社奇, 王国栋, 田鹏, 等.黄土高原刺槐人工林地土壤微生物的分布特征[J].水土保持学报,2004,18(6):128- 131.
[9]	ZHANG S Q,WANG G D, LIU Y P, et al. Distributive feature of soil microorganism of Pinus tabulaeformis plantation forestland in Loess Plateau[J].Acta Laser Biology Sinica, 2005,14(5):353- 358.
[9]	张社奇, 王国栋, 刘云鹏, 等. 黄土高原人工油松林地土壤微生物的分布特征[J]. 激光生物学报,2005,14(5):353- 358.
[10]	ZHENG X F, SU Y K , LIU B, et al. Microbial community diversity in tea root zone soils at different elevations [J]. Chinese Journal of Eco-Agriculture, 2010, 18(4): 866- 871.
[10]	郑雪芳,苏远科,刘波,等.不同海拔茶树根系土壤微生物群落多样性分析[J]. 中国生态农业学报, 2010, 18(4):866- 871.
[11]	LUO D, SHI Z M,TANG J C, et al. Soil microbial community structure of monoculture and mixed plantation stands of native tree species in south subtropical China[J].Chinese Journal of Applied Ecology, 2014,25(9): 2543- 2550.
[11]	BALKWILL D L, LEACH F R, WILSON J T, et al. Equivalence of microbial biomass measures based on membrane lipid and cell wall components, adenosine triphosphate, and direct counts in subsurface aquifer sediments [J]. Microbial Ecology, 1988, 16(1):73- 84.
[12]	YAO Q, SONG J, PAN F J, et al. Characteristics of phospholipid fatty acids of soil microorganism under different plant patterns of soybean[J]. Soybean Science, 2012, 34(3):26- 30.
[12]	罗达, 史作民, 唐敬超,等. 南亚热带乡土树种人工纯林及混交林土壤微生物群落结构[J]. 应用生态学报, 2014,25(9): 2543- 2550.
[13]	SUN L L. Community structure diversity of soil microbes under Phyllostachy pubescens stands revealed by PLFAs analysis[D]. Hangzhou: Zhejiang A F University, 2010.
[13]	姚钦, 宋洁, 潘凤娟,等. 磷脂脂肪酸技术在不同土地管理方式下土壤微生物多样性研究的应用[J]. 大豆科技, 2012,34(3):26- 30.
[14]	MA D L, ZANG S Y, LI Y. Microorganism in soil for Tricholoma matsutake growth based on phospholipid fatty biomarker method[J]. Guizhou Agricultural Science, 2015,43(3):106- 109.
[14]	孙棣棣. 应用磷脂脂肪酸方法研究毛竹林土壤微生物群落结构演变规律[D]. 杭州:浙江农林大学, 2010.
[15]	LI X, JIAO Y, YANG M D. Microbial diversity of different saline- alkaline soil analyzing by PLFA in the Hetao area of Inner Mongolia[J]. Ecological Science, 2014, 33(3): 488- 494.
[15]	马大龙, 臧淑英, 李莹. 基于磷脂脂肪酸生物标记法的松茸生长土壤微生物测定[J]. 贵州农业科学, 2015,43(3):106- 109.
[16]	WEI T X, YU X X, ZHU J Z, et al. Relationship between water supply and consumption of main planting tree species of protection forests in loess area of western Shanxi Province[J].Chinese Journal of Applied Ecology, 2001, 12(2):185- 189.
[16]	李新, 焦燕, 杨铭德. 用磷脂脂肪酸(PLFA)谱图技术分析内蒙古河套灌区不同盐碱程度土壤微生物群落多样性[J]. 生态科学, 2014, 33(3):488- 494.
[17]	魏天兴,余新晓,朱金兆,等.黄土区防护林主要造林树种水分供需关系研究[J].应用生态学报, 2001,12(2):185- 189.
[17]	WU D X,WEI W S,SONG C Y, et al. Quality assurance and quality control of terrestrial ecosystem biological observation data [M]. Beijing: China Environmental Science Press, 2012.
[18]	LIU B, HU G P, ZHENG X F, et al. Analysis on microbial diversity in the rhizosphere of rice by phospholipid fatty acids biomarkers [J]. Chinese Journal of Rice Science, 2010, 24(3):278- 288.
[18]	吴冬秀,韦文珊,宋创业,等. 陆地生态系统生物观测数据质量保证与质量控制[M].北京:中国环境科学出版社,2012.
[19]	BAI Q, GATTINGER A, ZELLES L. Characterization of microbial consortia in paddy rice soil by phospholipid analysis [J]. Microbial Ecology, 2000, 39(4):273- 281.
[19]	HU C J, GUO L, LIU G H. Soil microbial community structure under different vegetation restoration patterns in the loess hilly area[J]. Acta Ecologica Sinica,2014,34(11): 2986- 2995.
[20]	LIN S, ZHUANG J Q, CHEN T, et al. Microbial diversity in rhizosphere soils of different planting year tea trees: an analysis with phospholipid fatty acid biomarkers[J].Chinese Journal of Ecology,2013, 32(1):64- 71.
[20]	刘波, 胡桂萍, 郑雪芳,等. 利用磷脂脂肪酸(PLFAs)生物标记法分析水稻根际土壤微生物多样性[J]. 中国水稻科学, 2010, 24(3):278- 288.
[21]	WEI T X, ZHANG X J , ZHU J Z. The nutrient accumulation pattern and cycling in natural secondary forests in North China: a case study from the Caijiachuan Watershed, Shanxi Province[J]. Phyton, International Journal of Experimental Botany,2014, 83: 213- 223.
[21]	DONG L L, ZHENG F L, AN J. Characteristics of the soil microbial biomass in various land-uses in the loess hilly-gully region[J]. Chinese Journal of Soil Science, 2010, 41(6):1370- 1375.
[22]	GARLAND J L, MILLS A L. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization[J]. Applied and Environment Microbiology, 1991, 57: 2351- 2359.
[23]	胡婵娟, 郭雷, 刘国华. 黄土丘陵沟壑区不同植被恢复格局下土壤微生物群落结构[J]. 生态学报, 2014, 34(11): 2986- 2995.
[24]	林生, 庄家强, 陈婷,等. 不同年限茶树根际土壤微生物群落PLFA生物标记多样性分析[J]. 生态学杂志, 2013, 32(1):64- 71.
[25]	WAID J S. Does soil biodiversity depend upon metabiotic activity and influences?[J]. Applied Soil Ecology, 1999, 13(2):151- 158.
[26]	SAGGAR S, YEATES G W, SHEPHERD T G. Cultivation effects on soil biological properties, microfauna and organic matter dynamics in Eutric Gleysol and Gleyic Luvisol soils in New Zealand[J]. Soil Tillage Research, 2001, 58(1):55- 68.
[27]	董莉丽,郑粉莉,安娟.黄土丘陵区不同土地利用类型下土壤微生物生物量特征[J].土壤通报,2010,41(6):1370- 1375.

Cited By

Cited by

Periodical cited type(7)

1.	魏安琪，魏天兴，刘海燕，王莎. 黄土区刺槐和油松人工林土壤微生物PLFA分析. 北京林业大学学报. 2019(04): 88-98 . 本站查看
2.	李鹏飞，张兴昌，郝明德，崔勇兴，张燕江，朱世雷. 植被恢复对黄土高原矿区重构土壤理化性质、酶活性以及真菌群落的影响. 水土保持通报. 2019(05): 1-7 .
3.	陆梅，孙向阳，田昆，任玉连，王邵军，王行，彭淑娴. 纳帕海高原湿地不同退化阶段土壤真菌群落结构特征. 北京林业大学学报. 2018(03): 55-65 . 本站查看
4.	张蓉，于亚军. 煤矸山复垦林地和草地土壤微生物多样性和群落组成的差异及其影响因素. 生态学杂志. 2018(06): 1662-1668 .
5.	张树萌，黄懿梅，倪银霞，钟祺琪. 宁南山区人工林草对土壤真菌群落的影响. 中国环境科学. 2018(04): 1449-1458 .
6.	李敏敏，魏天兴，李信良，葛海潮. 黄土区蔡家川流域刺槐人工林林下物种多样性. 浙江农林大学学报. 2018(02): 227-234 .
7.	刘洋，曾全超，黄懿梅. 基于454高通量测序的黄土高原不同乔木林土壤细菌群落特征. 中国环境科学. 2016(11): 3487-3494 .

Other cited types(9)

Get Citation

PDF

XML

Article views (1621) PDF downloads (27) Cited by(16)

Turn off MathJax

Article Contents

Abstract

References

Soil microbial community structure and functional diversity in typical plantations marked by PLFA in hilly loess region

Abstract

References

Cited by

Periodical cited type(7)

Other cited types(9)

Catalog

Export File

Citation

Format

Content