Overexpression of KcTrxf in tobacco enhances salt tolerance through the regulation of ROS homeostasis under NaCl stress

JING Xiao-shu; SUN Yuan-ling; XIANG Min; QIAN Ze-yong; LANG Tao; ZHAO Rui; SHEN Xin; CHEN Shao-liang

doi:10.13332/j.1000-1522.20150010

Journal of Beijing Forestry University > 2015 > 37(6): 17-26. > DOI: 10.13332/j.1000-1522.20150010

JING Xiao-shu, SUN Yuan-ling, XIANG Min, QIAN Ze-yong, LANG Tao, ZHAO Rui, SHEN Xin, CHEN Shao-liang. Overexpression of KcTrxf in tobacco enhances salt tolerance through the regulation of ROS homeostasis under NaCl stress[J]. Journal of Beijing Forestry University, 2015, 37(6): 17-26. DOI: 10.13332/j.1000-1522.20150010

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Overexpression of KcTrxf in tobacco enhances salt tolerance through the regulation of ROS homeostasis under NaCl stress

1.
1 College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, P.R. China;
2.
2 College of Life Science, Hainan Normal University, Haikou, Hainan, 571158, P.R. China;

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Received Date: January 07, 2015

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Abstract

Abstract

Thioredoxins (Trxs)play a crucial role in mediating the redox state in living cells; however, little is known how Trxs mediate salt tolerance in woody species. The aim of this study was to clone putative Trxf from non-secretor mangrove Kandelia candel and analyze its role in NaCl tolerance. Salt treatment enhanced NPTs(non-protein thiols)contents and increased transcription of Trxf in K. candel. The KcTrxf gene contained an open reading frame of 585 bp encoding a putative thioredoxin protein with 194 amino acids. Sequence and localization analyses revealed that KcTrxf is a typical thioredoxin in the chloroplast. To clarify the role of KcTrxf in salinity tolerance, it was transferred to tobacco, a model experimental system for functional genetics. KcTrxf-transgenic lines were more salt tolerant than wild-type(WT)tobacco. In the WT tobacco, salinity(100 mmol/L NaCl)resulted in a marked reduction of chlorophyll content, and chlorophyll a/b ratio. The salt damage in WT resulted presumably from lipid peroxidation and ROS accumulation. However, the salt treatment caused less damage in KcTrxf-transgenic plants because of the up-regulated activity of catalase and ascorbate peroxidase. Moreover, KcTrxf-transgenic plants were able to increase the activities of MDAR(monodehydroascorbate reductase)and GR(glutathione reductase)in chloroplast AsA-GSH cycle, leading to an increase in the ratio of reduced glutathione(GSH)to oxidized glutathion(GSSG)and NPTs in the leaves. Therefore, KcTrxf-transgenic plants could better scavenge the salt-elicited reactive oxygen species(ROS)in leaf cells, compared to WT plants. In this way, reduction of chlorophyll content, and chlorophyll a/b ratio of KcTrxf-transgenic plants was consequently less restricted. As a result, KcTrxf -transgenic plants maintain photosynthesis and growth under salinity in the longer term.
- mangrove,
- Kandelia candel,
- thioredoxin,
- catalase,
- ascorbate peroxidase,
- AsA-GSH cycle,
- NaCl stress

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Overexpression of KcTrxf in tobacco enhances salt tolerance through the regulation of ROS homeostasis under NaCl stress

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