Mediation of NO on Cd2+ uptake in Populus euphratica cells under cadmium stress

WANG Shao-jie; ZHAO Nan; SHEN Ze-dan; SA Gang; SUN Hui-min; ZHAO Rui; SHEN Xin; CHEN Shao-liang

doi:10.13332/j.1000-1522.20150023

Journal of Beijing Forestry University > 2015 > 37(6): 11-16. > DOI: 10.13332/j.1000-1522.20150023

WANG Shao-jie, ZHAO Nan, SHEN Ze-dan, SA Gang, SUN Hui-min, ZHAO Rui, SHEN Xin, CHEN Shao-liang. Mediation of NO on Cd2+ uptake in Populus euphratica cells under cadmium stress[J]. Journal of Beijing Forestry University, 2015, 37(6): 11-16. DOI: 10.13332/j.1000-1522.20150023

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Mediation of NO on Cd2+ uptake in Populus euphratica cells under cadmium stress

College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, P.R. China;

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Received Date: January 20, 2015

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Abstract

We investigated the effect of nitric oxide (NO)on cadmium (Cd2+) tolerance in Populus euphratica cells.Results showed that 50 μmol/L Cd2+ significantly restricted cell growth; however, the inhibition of Cd2+ on cell growth was evidently alleviated by the addition of sodium nitroprusside (SNP, nitric oxide donor, 25 μmol/L). Moreover, SNP could relieve the decline of Cd2+-induced cell viability and damage to membrane during the period of cadmium stress. The effect of NO on cadmium kinetics was investigated using the non-invasive micro-test technique (NMT). After being subjected to 50 μmol/L CdCl2solution, P. euphratica showed a substantial Cd2+ influx. However, the cadmium-induced Cd2+ influx and the subsequent accumulation were both significantly restricted by 25 μmol/L SNP. Furthermore, pharmacological experiments showed that NO mediated the Cd2+ entry through calcium permeable channels in P.euphratica. The Cd2+ influx caused by Cd2+ stress was strongly inhibited by lanthanum chloride, a specific inhibitor of calcium channels. Noteworthy, NO was found to increase Ca2+ influx, which competitively inhibited the entry of Cd2+ through calcium permeable channels.Consequently, the Cd2+ uptake and buildup were limited, and thus the growth inhibition by cadmium was alleviated in P. euphratica cells.
- Populus euphratica cells,
- NO,
- cadmium stress,
- Cd2+ flux,
- Ca2+ flux,
- Ca2+ channel

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