Drought induces alterations in stomatal development in Populus deltoides&#x000D7;P. nigra

WANG Cong-peng; JIA Fu-li; LIU Sha; LIU Chao; XIA Xin-li; YIN Wei-lun

doi:10.13332/j.1000-1522.20160050

Journal of Beijing Forestry University > 2016 > 38(6): 28-34. > DOI: 10.13332/j.1000-1522.20160050

WANG Cong-peng, JIA Fu-li, LIU Sha, LIU Chao, XIA Xin-li, YIN Wei-lun. Drought induces alterations in stomatal development in Populus deltoides×P. nigra[J]. Journal of Beijing Forestry University, 2016, 38(6): 28-34. DOI: 10.13332/j.1000-1522.20160050

Citation:

PDF (1001 KB)

Drought induces alterations in stomatal development in Populus deltoides×P. nigra

National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, P. R. China.

More Information

Received Date: February 17, 2016
Revised Date: February 17, 2016
Published Date: June 29, 2016

Graphical Abstract

Abstract

Abstract

To explore the effect of drought to stomatal development in woody plants, five Populus deltoides× P. nigra clones, i.e., R270 (P. deltoides× P. nigra), NE-19 [P. nigra× (P. deltoides× P. nigra)], 107 (P.× euramericana ‘74/76'), 109 (P. deltoides× P. alba ‘Mincio') and 111 (P. deltoides× euramericana ‘Bellotto') were selected to undergo 14 days of drought and 7 days of re-watering treatment. The results showed that photosynthesis of NE-19, R270 and 107 returned to the normal level at the fifth day after re-watered, with stomatal conductance back to normal level after 3 days. However, 109 and 111 recovered slowly. As for the growth rate after re-watered, the order from high to low was NE-19 (64.96%), R270 (55.73%), 107(49.87%), 109(35.08%), and 111(23.62%). According the results of photosynthesis, stomatal conductance and growth rate, the drought tolerance was ranked as NE-19 > R270 > 107 > 109 > 111. For all five clones, the stomatal development slowed down when subjected to the drought, indicated by the stomatal index (SI) in young leaves. The SI of NE-19 and R270 had the most remarkable change during the drought, being 30.75% and 29.24%, respectively. The clones with better drought tolerance may be more positively responsive to ambient water content change through stomatal development. Using qRT-PCR we tested the difference of transcript abundance of five genes associated with stomatal development between control and drought treatment groups. EPFL9 and FAMA, which positively control stomatal development, were down-regulated during drought, but ERECTA and EPF1 which negatively regulate the process were up-regulated during drought, and SDD1 had no change during the whole treatment. Our findings highlight that poplar inhibits stomatal development through adjusting expression of ERECTA, EPF1, EPFL9 and FAMA in immature leaves to reduce water loss so as to withstand drought stress.
- Populus deltoids ×,
- Populus nigra,
- stomatal development,
- drought gene

FullText(HTML)

References (24)

References

[1]	COWAN I R, FARQUHAR G D. Stomatal function in relation to leaf metabolism and environment[J]. Symposia of the Society for Experimental Biology, 1977, 31(31):471-505.
[2]	CHAVES M M, MAROCO O P, PEREIRA O S. Understanding plant responses to drought: from genes to the whole plant[J]. Functional Plant Biology, 2003, 30(3):239-264.
[3]	PILLITTERI L J, DONG J. Stomatal development in Arabidopsis [J]. Arabidopsis Book, 2012, 11(1):e0066.
[4]	BERGMANN D, SACK F. Stomatal development[J]. Annual Review of Plant Biology, 2007, 58(4):163-181.
[5]	CASSON S A, HETHERINGTON A M. Environmental regulation of stomatal development[J]. Current Opinion in Plant Biology, 2009, 13(1):90-95.
[6]	KENTA H, RYOKO K, TORII K U, et al. The secretory peptide gene EPF1 enforces the stomatal one-cell-spacing rule[J]. Genes & Development, 2007, 21(14):1720-1725.
[7]	HARA K, YOKOO T, KAJITA R, et al. Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves[J]. Plant & Cell Physiology, 2009, 50(6):1019-1031.
[8]	TATSUHIKO K, RYOKO K, AYA M, et al. Stomatal density is controlled by a mesophyll-derived signaling molecule[J]. Plant & Cell Physiology, 2010, 51(1):1-8.
[9]	SUGANO S S, TOMOO S, YU I, et al. Stomagen positively regulates stomatal density in Arabidopsis [J]. Nature, 2010, 463:241-244.
[10]	NADEAU J A, SACK F D. Control of stomatal distribution on the Arabidopsis leaf surface[J]. Science, 2002, 296:1697-1700.
[11]	SHPAK E D, JESSICA M M, LYNN J P, et al. Stomatal patterning and differentiation by synergistic interactions of receptor kinases[J]. Pediatrics, 2005, 115(Suppl.4):1160-1164.
[12]	LEE J S, KUROHA T, HNILOVA M, et al. Direct interaction of ligand-receptor pairs specifying stomatal patterning[J]. Genes & Development, 2012, 26(2):126-136.
[13]	BERGER D, ALTMANN T. A subtilisin-like serine protease involved in the regulation of stomatal density and distribution in Arabidopsis thaliana [J]. Genes & Development, 2000, 14(9):1119-1131.
[14]	URITZA V G, DIETER B, THOMAS A. The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development[J]. Plant Cell, 2002, 14(7):1527-1539.
[15]	BERGMANN D C, WOLFGANG L, SOMERVILLE C R. Stomatal development and pattern controlled by a MAPKK kinase[J]. Science, 2004, 304:1494-1497.
[16]	PILLITTERI L, TORII K. Breaking the silence: three bHLH proteins direct cell-fate decisions during stomatal development[J]. Bioessays, 2007, 29(9):861-70.
[17]	WANG H, NGWENYAMA N, LIU Y, et al. Stomatal development and patterning are regulated by environmentally responsive mitogen-activated protein kinases in Arabidopsis [J]. Plant Cell, 2007, 19(1):63-73.
[18]	MIYAZAWA S I, TURPIN D H. Stomatal development in new leaves is related to the stomatal conductance of mature leaves in poplar ( Populus trichocarpa×P. deltoides )[J]. Journal of Experimental Botany, 2006, 57(2):373-380.
[19]	SAKURAI N, AKIYAMA M, KURAISHI S. Irreversible effects of water stress on growth and stomatal development in cotyledons of etiolated squash seedlings[J]. Plant & Cell Physiology, 1986, 27(6):1177-1185.
[20]	QUARRIE S A, JONES H G. Effects of abscisic acid and water stress on development and morphology of wheat [J]. Journal of Experimental Botany, 1977, 28(1): 192-203.
[21]	XU Z, ZHOU G. Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass[J]. Journal of Experimental Botany, 2008, 59(12): 3317-3325.
[22]	CLIFFORD S C, BLACK C R, ROBERTS J A, et al. The effect of elevated atmospheric CO 2 and drought on stomatal frequency in groundnut ( Arachis hypogaea (L.))[J]. Journal of Experimental Botany, 1995, 46(288): 847-852.
[23]	CASSON S, GRAY J E. Influence of environmental factors on stomatal development[J]. New Phytologist, 2008, 178(1): 9-23.
[24]	SHIMADA T, SUGANO S S, HARA-NISHIMURA I. Positive and negative peptide signals control stomatal density[J]. Cellular and Molecular Life Sciences, 2011, 68(12): 2081-2088.

Cited By

Cited by

Periodical cited type(2)

1.	朱济友，徐程扬，吴鞠. 基于eCognition植物叶片气孔密度及气孔面积快速测算方法. 北京林业大学学报. 2018(05): 37-45 . 本站查看
2.	贾伏丽，王丛鹏，刘沙，焦志银，尹伟伦，夏新莉. 外源BR与IAA对欧美杨耐旱性的影响. 北京林业大学学报. 2017(07): 31-39 . 本站查看

Other cited types(2)

Get Citation

PDF

XML

Article views (1696) PDF downloads (23) Cited by(4)

Turn off MathJax

Article Contents

Abstract

References

Drought induces alterations in stomatal development in Populus deltoides×P. nigra

Abstract

References

Cited by

Periodical cited type(2)

Other cited types(2)

Catalog

Export File

Citation

Format

Content