Relationships between pathogenic bacterium and the endophytic bacteria isolated from Bursaphelenchus xylophilus.

CAO Feng; YANG Ling; GONG Shu-rong; ZHANG Lin; NIU Qiu-hong

doi:10.13332/j.1000-1522.20160082

Journal of Beijing Forestry University > 2016 > 38(9): 25-33. > DOI: 10.13332/j.1000-1522.20160082

CAO Feng, YANG Ling, GONG Shu-rong, ZHANG Lin, NIU Qiu-hong. Relationships between pathogenic bacterium and the endophytic bacteria isolated from Bursaphelenchus xylophilus.[J]. Journal of Beijing Forestry University, 2016, 38(9): 25-33. DOI: 10.13332/j.1000-1522.20160082

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Relationships between pathogenic bacterium and the endophytic bacteria isolated from Bursaphelenchus xylophilus.

1.
1 School of Life Science and Technology,Nanyang Normal University, Nanyang, Henan, 473061, P. R. China;
2.
2 Nanyang Academy of Agricultural Sciences, Nanyang, Henan, 473083, P. R. China.

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Received Date: March 13, 2016
Published Date: September 29, 2016

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Abstract

Abstract

The metagenome of ecosystem of endophytic bacteria associated with the pine wood nematode Bursaphelenchus xylophilus was analyzed by 16S rRNA gene library and illumina Miseq sequencing. The results showed that the dominant bacteria were Xanthomonadaceae (22.41%), Sphingobacteriaceae (17.66%), Comamonadaceae (14.71%) and Rhizobiaceae (10.17%). Five endophytic bacteria were isolated from B. xylophilus using dilution plate method. These bacteria were classified by phenotypic and genotypic characteristics. The five endophytic bacteria all showed nematocidal activities in vitro. The bacterium Klebsiella sp. sct5 showed highest nematocidal activity, and 100% of the tested B. xylophilus were killed within 4 days. Furthermore, the strain sct5 could produce proteases and form biofilm quickly in vitro. Moreover, the bacterial strain sct5 had compatible relationship with Bacillus nematocida B16. The compatible combination of sct5 with B16 resulted in enhanced nematocidal activities within 1 d (75％) against Bursaphelenchus xylophilus as compared to the single strain sct5 (60％) or B16 (40％). In contrast, the incompatible combination of sct29 with B16 led to reduced disease nematocidal activities (only 13％ within 1 d). This study firstly reported nematocidal activities of endophytic bacteria isolated from Bursaphelenchus xylophilus and their relationships with pathogenic bacteria Bacillus nematocida B16, which lays theoretical foundations for developing co-inoculated bacterial strains in biocontrol and improving the stability of bio-control strains. Also, our research explains the relationships between endophytic bacteria in Bursaphelenchus xylophilus and pathogenic bacteria from a new perspective.
- Metagenome,
- Bursaphelenchus xylophilus,
- endophytic bacteria,
- Bacillus nematocida B16,
- interaction

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References (35)

References

[1]	YANG B J, PAN H Y, TANG J, et al. Pine wilt disease [M]. Beijing: Chinese Forestry Press, 2003.
[1]	杨宝君,潘宏阳,汤坚,等.松材线虫病[M].北京:中国林业出版社,2003.
[2]	柴希民,蒋平.松材线虫病的发生与防治[M].北京:中国农业出版社,2003.
[3]	CHAI X M, JIANG P. Occurrence and prevention for pine wilt disease[M].Beijing: Chinese Agriculture Press, 2003.
[4]	曾腓力,贲爱玲,郑敬荣,等. 美国松材线虫体表携带优势细菌的鉴定及致病性[J].浙江农林大学学报,2012, 29(5): 696-702.
[5]	ZENG F L, BEN A L, ZHENG J R, et al. Identification and pathogenicity of bacterial strains carried by American pine wood nematodes [J]. Journal of Zhejiang AF University, 2012, 29(5): 696-702.
[6]	刘侃诚,曹东霞,田兴一,等. 松材线虫与拟松材线虫杂交及后代繁殖力[J].东北林业大学学报,2015, 43 (11):103-105.
[7]	LIU K C, CAO D X, TIAN X Y, et al. Hybridization between Bursaphelenchus xylophilus and B. mucronatus and reproduction of the offspring [J]. Journal of Northeast Forestry University, 2015, 43 (11):103-105.
[8]	谈家金,冯志新.松材线虫病病理生理学研究进展[J]. 江西科学,2001, 19(1):27-30.
[9]	TAN J J, FENG Z X. Advance on physiopathology of pine wood nematode disease[J]. Jiangxi Science, 2001, 19(1):27-30.
[10]	孙永春.南京中山陵发现松材线虫[J].江苏林业科技,1982, 12 (4): 47.
[11]	SUN Y C. Bursaphelenchus xylophilus was found in Nanjing Sun Yat-sens Mausoleum[J]. Jiangsu Forest Science and Technology, 1982, 12 (4): 47.
[12]	朱克恭,朱正昌,严敖金. 松材线虫病的流行与研究进展[C]∥杨宝君. 中国松材线虫病的流行与治理.北京:中国林业出版社,1995: 297-314.
[13]	ZHU K G, ZHU Z C, YAN A J. Popular and research progress of Bursaphelenchus xylophilus[C]∥Popular and administration of pine wood nematode disease in China. Beijing: Chinese Forestry Press, 1995: 297-314.
[14]	NIU Q H, HUANG X W, ZHANG L, et al. A trojan horse mechanism of bacterial pathogenesis against nematodes [J]. Proceedings of the National Academy of Sciences, 2010, 107(38):16631-16636.
[15]	GUARNER F, MALAGELAD J R. Gut flora in health and disease[J]. The Lancet, 2003, 361: 512-19.
[16]	FORST S, DOWELS B,BOEMARE N, et al. Xenorhabdus and Photorhabdus spp. bugs that kill bugs[J]. Annual Reviews in Microbiology, 1997, 51(1) : 47-72.
[17]	NOUR S M, LAWRENCE J R, ZHU H,et al. Bacteria associated with cysts of the soybean cyst nematode (HeLerodera glycines) [J]. Applied and Environmental Microbiology, 2003, 69(1): 607-615.
[18]	HAEGEMAN A,VANHOLME B,JACOB J, et al. An endosymbiotic bacterium in a plant-parasitic nematode: member of a new Wolbachia supergroup[J]. International Journal for Parasitology, 2009, 39 (9):1045-1054.
[19]	袁为敏,吴小芹,叶建仁,等. 松材线虫和拟松材线虫体内细菌的透射电镜观察及分离鉴定[J]. 微生物学报, 2011, 51(8): 1071-1077.
[20]	YUAN W M, WU X Q, YE J R, et al. Observation by transmission electron microscope and identification of endophytic bacteria isolated from Bursaphelenchus xylophilus and B. mucronatus[J]. Acta Microbiologica Sinica, 2011, 51(8): 1071-1077.
[21]	ROSEN, S, SJOLLEMA K, VEEHUIS M, et al. A cytoplasmic lectin produced by the fungus Arthobotrys oligospora functions as a storage protein during saprophytic and parasitic growth[J]. Microbiology, 1997, 143(8): 2593-2604.
[22]	杨维青,刘晓峰,黄震.铜绿假单胞菌临床菌株生物被膜的定量分析[J].中国抗生素杂志,2009,34(3):181-183.
[23]	YANG W Q, LIU X F, HUANG Z. Quantification for biofilm-forming of Pseudomonas aeruginosa isolate[J].Journal of Antibiotics in China, 2009,34(3):181-183.
[24]	陈志谊,刘邮洲,刘永锋, 等.拮抗细菌菌株间的互作关系及其对生物防治效果的影响[J].植物病理学报,2005,35(6): 539-544.
[25]	CHEN Z Y, LIU Y Z, LIU Y F, et al. Compatibility between antagonistic bacterial strains and biocontrol efficacy by different bacterial combinations against Fusarium wilt of vegetables[J]. Acta Phytopathologica Sinica, 2005, 35(6):539-544.
[26]	XU P,LI W J,XU L H,et al. A microwave-based method for genomic DNA extraction from Actinomycetes[J]. Microbiology,2003,30 (4) : 82-84.
[27]	THOMPSON J,GIBSON T J,PLEWNIAK F,et al. The clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Research,1997,25(24):4876-4882.
[28]	KUMAR S,TAMURA K,NEI M.MEGA 3: integrated software for molecular evolutionary genetics analysis and sequence alignment[J]. Briefings in Bioinformatics, 2004, 5(2):150-163.
[29]	SCHLOSS P D, HANDELSMAN J. Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness[J]. Applied and Environmental Microbiology, 2005, 71(3):1501-6.
[30]	潘沧桑. 松材线虫病研究进展(综述)[J]. 厦门大学学报(自然科学版),2011,50(2):476-483.
[31]	PAN C S. Development of studies on pinewood nematodes diseases[J]. Journal of Xiamen University (Natural Science), 2011,50(2):476-483.
[32]	田雪亮,张祺玲,陈国华,等. 松材线虫伴生细菌多样性的宏基因组分析[J]. 微生物学报, 2010, 50(7):909-916.
[33]	TIAN X L, ZHANG Q L, CHEN G H, et al. Diversity of bacteria associated with pine wood nematode revealed by metagenome[J]. Acta Microbiologica Sinica, 2010, 50(7):909-916.
[34]	NIU Q H, ZHANG L, ZHANG K Q, et al. Changes in intestinal microflora of Caenorhabditis elegans following Bacillus nematocida B16 infection[J]. Scientific Reports, 2016, 6[2016-02-28]. DOI: 10.1038/srep20178.

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