Microbial and enzyme activities in rhizosphere soil of different forest stand in karst and non karst areas

Fan Zhouzhou; Lu Shuyu; Wang Jiao; Guo Dongchu; Pang Danbo; Zhou Jinxing; Peng Xiawei

doi:10.13332/j.1000-1522.20180011

Journal of Beijing Forestry University > 2018 > 40(7): 55-61. > DOI: 10.13332/j.1000-1522.20180011

Fan Zhouzhou, Lu Shuyu, Wang Jiao, Guo Dongchu, Pang Danbo, Zhou Jinxing, Peng Xiawei. Microbial and enzyme activities in rhizosphere soil of different forest stand in karst and non karst areas[J]. Journal of Beijing Forestry University, 2018, 40(7): 55-61. DOI: 10.13332/j.1000-1522.20180011

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Microbial and enzyme activities in rhizosphere soil of different forest stand in karst and non karst areas

1.
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
2.
School of Soil and Water Conservation, Beijing Forestry University, Beijing 100083, China

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Received Date: January 08, 2018
Revised Date: March 26, 2018
Published Date: June 30, 2018

Graphical Abstract

Abstract

Abstract

ObjectiveThis paper aims to clarify the effects of different forests on soil microorganism and soil enzyme activity under different geological conditions, providing a theoretical basis for further research on restoration of natural ecological system.
MethodThe soil samples were collected separately in rhizosphere of Eucalyptus robusta plantation, Pinus yunnanensis plantation and natural secondary forest growing up in karst and non-karst areas of Jianshui, Yunnan of southwestern China. The correlations between microorganisms and soil enzymes from different geological backgrounds and forests were studied by dilution coating and soil enzyme measurement.
ResultThe results showed that there was a significant difference between the number of actinomycetes in the rhizosphere soil of E. robusta and P. yunnanensis forests under the same geological background. The microbial quantity of secondary forest in karst area was 18.2×10⁵ cfu/g. Generally, influenced by the restoration patterns and geological conditions, the activities of soil enzymes were different. Correlation analysis showed that urease and catalase activity were positively correlated with the number of actinomycetes. There were interrelations between urease, acid phosphatase, carbonic anhydrase and catalase.
ConclusionThe results reveal that geological backgrounds, forest stand and the restoration patterns will affect soil microbial community composition and transformation of soil substance.
- karst,
- soil enzyme,
- rhizosphere,
- soil microorganism

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[1]	邱权, 李吉跃, 王军辉, 等.西宁南山4种灌木根际和非根际土壤微生物、酶活性和养分特征[J].生态学报, 2014, 34(24): 7411-7420. http://d.old.wanfangdata.com.cn/Periodical/stxb201424029 Qiu Q, Li J Y, Wang J H, et al. Microbes, enzyme activities and nutrient characteristics of rhizosphere and nonrhizosphere soils under four shrubs in Xining Nanshan Prefecture, China[J]. Acta Ecologica Sinica, 2014, 34(24): 7411-7420. http://d.old.wanfangdata.com.cn/Periodical/stxb201424029
[2]	倪彬.巨桉人工林根系土壤微生物、根系土壤酶活性与根系土壤养分研究[D].雅安: 四川农业大学, 2007. Ni B. Study on rhizosphere soil microorganism and rhizosphere soil enzyme activity and rhizosphere soil nitrient in Eucalyptus grandis plantation[D].Yaan: Sichuan Agricultural University, 2007.
[3]	侯本栋, 马风云, 宋玉民, 等.不同树种对土壤养分、酶活性与微生物影响的研究[J].江西农业大学学报, 2006, 28(5): 734-738. doi: 10.3969/j.issn.1000-2286.2006.05.020 Hou B D, Ma F Y, Song Y M, et al. A study on effects of different tree species on soil nutrients, enzyme activities and microorganisms[J]. Acta Agriculturae Universitatis Jiangxiensis, 2006, 28(5): 734-738. doi: 10.3969/j.issn.1000-2286.2006.05.020
[4]	陈家瑞, 曹建华, 李涛, 等.西南典型岩溶区土壤微生物数量研究[J].广西师范大学学报(自然科学版), 2010, 28(4): 96-100. doi: 10.3969/j.issn.1001-6600.2010.04.021 Chen J R, Cao J H, Li T, et al. Soil microorganisms in typical karst region of southwest China[J]. Journal of Guangxi Normal University(Natural Science Edition), 2010, 28(4): 96-100. doi: 10.3969/j.issn.1001-6600.2010.04.021
[5]	高喜, 万珊, 曹建华, 等.岩溶区与非岩溶区土壤微生物活性的对比研究[J].地球与环境, 2012, 40(4): 499-504. http://d.old.wanfangdata.com.cn/Periodical/dzdqhx201204005 Gao X, Wan S, Cao J H, et al. Comparative investigation of soil microbial activity in the karst and non-karst areas[J]. Earth and Environment, 2012, 40(4): 499-504. http://d.old.wanfangdata.com.cn/Periodical/dzdqhx201204005
[6]	赵维娜, 王艳霞, 陈奇伯.高山栎天然林土壤酶活性与土壤理化性质和微生物数量的关系[J].东北林业大学学报, 2015, 43(9): 72-77. doi: 10.3969/j.issn.1000-5382.2015.09.015 Zhao W N, Wang Y X, Chen Q B. Relationships between the soil enzyme activity, physical chemical properties and microorganism quantity in Quercus aquifolioides forest[J]. Journal of Northeast Forestry University, 2015, 43(9): 72-77. doi: 10.3969/j.issn.1000-5382.2015.09.015
[7]	鲍士旦.土壤农化分析[M].北京:中国农业出版社, 2000. Bao S D. Soil agricultural chemistry analysis[M]. Beijing: China Agriculture Press, 2005.
[8]	姚槐应.土壤微生物生态学及其实验技术[M].北京:科学出版社, 2006. Yao H Y. Soil microbial ecology and its experimental techniques[M]. Beijing: Science Press, 2006.
[9]	李睿玉, 王煜, 王亚男, 等.土荆芥不同发育期根际土壤养分、酶活及微生物数量的变化[J].生态环境学报, 2014, 23(9): 1526-1530. doi: 10.3969/j.issn.1674-5906.2014.09.020 Li R Y, Wang Y, Wang Y N, et al. Changes of soil nutrient, soil enzymes activities and microbial communities in rhizosphere soil of Chenopodium ambrosioides L. at different development phases[J]. Ecology and Environmental Sciences, 2014, 23(9): 1526-1530. doi: 10.3969/j.issn.1674-5906.2014.09.020
[10]	晋曦.不同封育年限下石灰岩山地土壤微生物及酶活性研究[D].合肥: 安徽农业大学, 2014. Jin X. Dynamics of soil microbial activities following mountan closing[D]. Hefei: Anhui Agricultural University, 2014.
[11]	涂志华.沿海防护林23个竹种根际土壤酶活性与微生物的研究[D].福州: 福建农林大学, 2012. Tu Z H. Study on rhziosphere soil enzyme activity and microorganism of 23 bamboos in costal shelter-forest[D].Fuzhou: Fujian Agriculture and Forestry University, 2012.
[12]	李元, 牛文全, 张明智, 等.加气灌溉对大棚甜瓜土壤酶活性与微生物数量的影响[J].农业机械学报, 2015, 46(8): 121-129. http://d.old.wanfangdata.com.cn/Periodical/nyjxxb201508018 Li Y, Niu W Q, Zhang M Z, et al. Effects of aeration on rhizosphere soil enzyme activities and soil microbes for muskmelon in plastic greenhouse[J]. Transactions of the Chinese Society for Agricultural Machinery, 2015, 46(8): 121-129. http://d.old.wanfangdata.com.cn/Periodical/nyjxxb201508018
[13]	李国梁.柏木低效林不同改造模式土壤微生物和土壤酶活性研究[D].雅安: 四川农业大学, 2012. Li G L. Study on enzyme activity and soil microorganisms in different transformation pattern of the cypress inefficient[D]. Yaan: Sichuan Agricultural University, 2012.
[14]	吴雁, 张金池.微生物碳酸酐酶在岩溶系统碳循环中的作用与应用研究进展[J].生物学杂志, 2015, 32(3): 78-83. doi: 10.3969/j.issn.2095-1736.2015.03.078 Wu Y, Zhang J C. Microbial carbonic anhydrase action and application on carbon cycling in karst dynamic system: a review[J]. Journal of Biology, 2015, 32(3): 78-83. doi: 10.3969/j.issn.2095-1736.2015.03.078
[15]	Li W, Yu L J, Yuan D X, et al. A study of the activity and ecological significance of carbonic anhydrase from soil and its microbes from different karst ecosystems of southwest China[J]. Plant & Soil, 2005, 272(1-2): 133-141.
[16]	王静, 徐广平, 曾丹娟, 等.岩溶区和非岩溶区两种优势植物凋落叶分解的比较研究[J].广西植物, 2013, 33(3): 338-345. doi: 10.3969/j.issn.1000-3142.2013.03.010 Wang J, Xu G P, Zeng D J, et al. Comparative study on decomposition of leaf litters from two dominant species under karst and non-karst terrains[J]. Guihaia, 2013, 33(3): 338-345. doi: 10.3969/j.issn.1000-3142.2013.03.010
[17]	赵汝东, 樊剑波, 何园球, 等.红壤丘陵区人工林恢复措施对土壤酶活性和微生物学性质的影响[J].土壤, 2012, 44(4): 576-580. doi: 10.3969/j.issn.0253-9829.2012.04.008 Zhao R D, Fan J B, He Y Q, et al. Effects of plantation restoration approaches on soil enzyme activities and microbial properties in hilly red soil region[J]. Soil, 2012, 44(4): 576-580. doi: 10.3969/j.issn.0253-9829.2012.04.008
[18]	康冰, 刘世荣, 蔡道雄, 等.南亚热带不同植被恢复模式下土壤理化性质[J].应用生态学报, 2010, 21(10): 2479-2486. http://d.old.wanfangdata.com.cn/Periodical/yystxb201010005 Kang B, Liu S R, Cai D X, et al. Soil physical and chemical characteristics under different vegetation restoration patterns in China south subtropical area[J]. Chinese Journal of Applied Ecology, 2010, 21(10): 2479-2486. http://d.old.wanfangdata.com.cn/Periodical/yystxb201010005
[19]	Burghelea C, Zaharescu D G, Dontsova K, et al. Mineral nutrient mobilization by plants from rock: influence of rock type and arbuscular mycorrhiza[J]. Biogeochemistry, 2015, 124(1-3): 187-203. doi: 10.1007/s10533-015-0092-5
[20]	罗达, 史作民, 唐敬超, 等.南亚热带乡土树种人工纯林及混交林土壤微生物群落结构[J].应用生态学报, 2014, 25(9): 2543-2550. http://d.old.wanfangdata.com.cn/Periodical/yystxb201409012 Luo D, Shi Z M, Tang J C, et al. Soil microbial community structure of monoculture and mixed plantation stands of native tree species in south subtropical China[J]. Chinese Journal of Applied Ecology, 2014, 25(9): 2543-2550. http://d.old.wanfangdata.com.cn/Periodical/yystxb201409012
[21]	连宾, 袁道先, 刘再华.岩溶生态系统中微生物对岩溶作用影响的认识[J].科学通报, 2011, 56(26): 2158-2161. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=kxtb201126002 Lian B, Yuan D X, Liu Z H. Effect of microbes on karstification in karst ecosystems[J]. Chinese Science Bulletin, 2011, 56(26): 2158-2161. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=kxtb201126002
[22]	栾丽英, 房玉林, 宋士任, 等.不同树龄酿酒葡萄不同土壤深度根际和根区微生物数量的研究[J].西北林学院学报, 2009, 24(2): 37-41. http://d.old.wanfangdata.com.cn/Periodical/xblxyxb200902008 Luan L Y, Fang Y L, Song S R, et al. Soil microbe in vineyards with different tree ages and different soil depths[J]. Journal of Northwest Forestry University, 2009, 24(2): 37-41. http://d.old.wanfangdata.com.cn/Periodical/xblxyxb200902008
[23]	Yuan X F, Xu J, Chai H, et al. Differences of rhizo-bacterial diversity and the content of peimine and peiminine of Fritillaria thunbergii among different habits[J]. Journal of Medicinal Plant Research, 2010, 4(6): 465-470.
[24]	龙健, 李娟, 江新荣, 等.贵州茂兰喀斯特森林土壤微生物活性的研究[J].土壤学报, 2004, 41(4): 597-602. doi: 10.3321/j.issn:0564-3929.2004.04.016 Long J, Li J, Jiang X R, et al. Soil microbial activities in Maolan karst forest, Guizhou Province[J]. Acta Pedologica Sinica, 2004, 41(4): 597-602. doi: 10.3321/j.issn:0564-3929.2004.04.016
[25]	司登宇.凤阳山不同林分类型土壤生物活性研究[D].南京: 南京林业大学, 2013. Si D Y. Study on soil biological activity of different forest types in Fengyang Mountain[D]. Nanjing : Nanjing Forestry University, 2013.
[26]	陈汝, 王海宁, 姜远茂, 等.不同苹果砧木的根际土壤微生物数量及酶活性[J].中国农业科学, 2012, 45(10): 2099-2106. doi: 10.3864/j.issn.0578-1752.2012.10.024 Chen R, Wang H N, Jiang Y M, et al. Rhizosphere soil microbial quantity and enzyme activity of different apple rootstocks[J]. Scientia Agricultura Sinica, 2012, 45(10): 2099-2106. doi: 10.3864/j.issn.0578-1752.2012.10.024
[27]	Colemanderr D, Desgarennes D, Fonsecagarcia C, et al. Plant compartment and biogeography affect microbiome composition in cultivated and native agave species[J]. New Phytologist, 2016, 209(2): 798-811. doi: 10.1111/nph.13697

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Microbial and enzyme activities in rhizosphere soil of different forest stand in karst and non karst areas