Expression profiles and regulation of FT/TFL1-like genes in Pinus tabuliformis

Liu Yang; Li Bangtong; Du Guihua; Huang Dongxu; Zhou Xianqing; Niu Shihui; Li Wei

doi:10.13332/j.1000-1522.20180040

Journal of Beijing Forestry University > 2018 > 40(10): 60-66. > DOI: 10.13332/j.1000-1522.20180040

Liu Yang, Li Bangtong, Du Guihua, Huang Dongxu, Zhou Xianqing, Niu Shihui, Li Wei. Expression profiles and regulation of FT/TFL1-like genes in Pinus tabuliformis[J]. Journal of Beijing Forestry University, 2018, 40(10): 60-66. DOI: 10.13332/j.1000-1522.20180040

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Expression profiles and regulation of FT/TFL1-like genes in Pinus tabuliformis

1.
National Engineering Laboratory for Forest Tree Breeding, Beijing Forestry University, Beijing 100083, China
2.
Forestry Seedling Management Station, Taiyuan 030001, Shanxi, China
3.
Agriculture and Animal Husbandry Bureau, Ningcheng 024200, Inner Mongolia, China
4.
National Fine Seed Base of Pinus tabuliformis, Qigou State-owned Forest Farm, Pingquan 067509, Hebei, China

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Received Date: January 29, 2018
Revised Date: April 24, 2018
Published Date: September 30, 2018

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Abstract

Abstract

ObjectiveThere are three subfamilies of the PEBP gene family:FT-like, TFL1-like and FT/TFL11-like. We have known that FT/TFL1-like genes are the core regulation hubs in reproductive regulation network in angiosperms. Only the undifferentiated FT/TFL1-like subfamily in conifers was suggested play important roles of reproduction, growth rhythm and dormancy process. However, the knowledge of its expression profiles and the expressional regulation by different environmental factors about the FT/TFL1-like genes in conifers are still limited. This may result in some inaccurate speculation about the role of its related expression mechanism.
MethodIn this study, we isolated two FT/TFL1-like genes form Chinese pine (Pinus tabuliformis), named as PtTFL1 and PtTFL2. The expression level of these genes in different tissues, the developing male cones and female cones in different stage, the needles during the dormancy and dormancy release, the response to relative warmer temperature and under different day length and light were studied.
ResultThe results showed that PtTFL1 was specifically expressed in pollen and nearly no expression in other tissues. But the PtTFL2 was very broadly expressed and significantly expressed in the bud tissue than other tissues. But both of two genes were not significantly expressed in the rapidly proliferating callus; in addition, the analysis of the expression level of PtTFL2 in different environments was found. PtTFL2 accumulated in buds and conifers during the dormancy period and decreased when dormancy release; under the condition of relatively high temperature but not enough to break dormancy, the expression of PtTFL2 was significantly inhibited, which was similar to that of far red light treatment.
ConclusionIt can be seen that PtTFL1 may be related to pollen maturation process in the reproductive development process of Pinus tabuliformis, but PtTFL2 may be involved in more other developmental regulation, both of them are not necessary to maintain cell activity. The autumn environmental conditions are very conducive to the accumulation of PtTFL2 in male, female cones and needle, this expression profile of it is highly correlated with dormancy. However, PtTFL2 expression pattern is too sensitive to temperature and the expression patterns of male, female cones and needles are more likely to result in response to temperature, indicating that PtTFL2 itself probably is only a temperature response and signal transmission factor and regulated by far-red light under short day rather than related to the regulation of cone development or be sufficient to maintain dormancy. This study provides important data for further understanding the function of FT/TFL1-like gene in important developmental processes, such as reproductive and dormancy of Chinese pine, and its expression regulation by different environmental factors.
- FT/TFL1-like gene,
- PtTFL2,
- expression profiles,
- expressional regulation,
- Pinus tabuliformis

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[1]	张云中, 祁丽君, 王沙生.油松球花发端期的研究[J].北京林业大学学报, 1990, 12(4): 57-62. http://www.cnki.com.cn/Article/CJFDTOTAL-BJLY199004008.htm Zhang Y Z, Qi L J, Wang S S. The study of cone initiation period in Pinus tabuliformis[J]. Journal of Beijing Forestry University, 1990, 12(4): 57-62. http://www.cnki.com.cn/Article/CJFDTOTAL-BJLY199004008.htm
[2]	Bluemel M, Dally N, Jung C. Flowering time regulation in crops: what did we learn from Arabidopsis?[J]. Current Opinion in Biotechnology, 2015, 32: 121-129. doi: 10.1016/j.copbio.2014.11.023
[3]	Hanzawa Y, Money T, Bradley D. A single amino acid converts a repressor to an activator of flowering[J]. Proceedings of the National Academy of Sciences, 2005, 102(21): 7748-7753. doi: 10.1073/pnas.0500932102
[4]	Liu Y Y, Yang K Z, Wei X X, et al. Revisiting the phosphatidylethanolamine-binding protein (PEBP) gene family reveals cryptic FLOWERING LOCUS T gene homologs in gymnosperms and sheds new light on functional evolution[J]. New Phytologist, 2016, 212(3): 730-744. doi: 10.1111/nph.14066
[5]	Nystedt B, Street N R, Wetterbom A, et al. The Norway spruce genome sequence and conifer genome evolution[J]. Nature, 2013, 497: 579-584. doi: 10.1038/nature12211
[6]	Gyllenstrand N, Clapham D, Källman T, et al. A Norway spruce FLOWERING LOCUS T homologs implicated in control of growth rhythm in conifers[J]. Plant Physiology, 2007, 144(1): 248-257. https://www.jstor.org/stable/40065336
[7]	Karlgren A, Gyllenstrand N, Källman T, et al. Evolution of the PEBP gene family in plants: functional diversification in seed plant evolution[J/OL]. Plant Physiology, 2011[2017-10-21]. https://doi.org/10.1104/pp.111.176206.
[8]	Klintenäs M, Pin P A, Benlloch R, et al. Analysis of conifer FLOWERING LOCUS T/TERMINAL FLOWER1-like genes provides evidence for dramatic biochemical evolution in the angiosperm FT lineage[J]. New Phytologist, 2012, 196(4): 1260-1273. doi: 10.1111/j.1469-8137.2012.04332.x
[9]	Asante D K A, Yakovlev I A, Fossdal C G, et al. Gene expression changes during short day induced terminal bud formation in Norway spruce[J]. Plant, Cell & Environment, 2011, 34(2): 332-346. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=24fbeebe42c1ac5fbeae811a046b87af
[10]	Karlgren A, Gyllenstrand N, Clapham D, et al. FLOWERING LOCUS T/TERMINAL FLOWER₁-like genes affect growth rhythm and bud set in Norway spruce[J]. Plant Physiology, 2013, 163(2): 792-803. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=74fdce970d160a2e5d4d6622bf77528c
[11]	Thompson J D, Gibson T J, Higgins D G. Multiple sequence alignment using ClustalW and ClustalX[J/OL]. Current Protocols in Bioinformatics, 2003[2017-10-21]. http://doi.org/10.1002/0471250953.bi0203s00.
[12]	Sohpal V K, Dey A, Singh A. MEGA biocentric software for sequence and phylogenetic analysis: a review[J]. International Journal of Bioinformatics Research and Applications, 2010, 6(3): 230-240. http://cn.bing.com/academic/profile?id=f85918849d9d9ce71ab2c4888bc9cffc&encoded=0&v=paper_preview&mkt=zh-cn
[13]	Niu S H, Yuan H W, Sun X R, et al. A transcriptomics investigation into pine reproductive organ development[J]. New Phytologist, 2016, 209(3): 1278-1289. doi: 10.1111/nph.2016.209.issue-3
[14]	Bray N L, Pimentel H, Melsted P, et al. Near-optimal probabilistic RNA-seq quantification[J]. Nature Biotechnology, 2016, 34(5): 525-527. doi: 10.1038/nbt.3519
[15]	Ho W W H, Weigel D. Structural features determining flower-promoting activity of Arabidopsis FLOWERING LOCUS T[J/OL]. The Plant Cell, 2014[2017-10-06]. https://doi.org/10.1105/tpc.113.115220.
[16]	Singh R K, Svystun T, AlDahmash B, et al. Photoperiod-and temperature-mediated control of phenology in trees-a molecular perspective[J]. New Phytologist, 2017, 213(2): 511-524. doi: 10.1111/nph.14346
[17]	Bouché F, D'Aloia M, Tocquin P, et al. Integrating roots into a whole plant network of flowering time genes in Arabidopsis thaliana[J]. Scientific Reports, 2016, 6(4): 29042. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926122/
[18]	Corbesier L, Vincent C, Jang S, et al. FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis[J]. Science, 2007, 316: 1030-1033. doi: 10.1126/science.1141752
[19]	Takala H, Björling A, Berntsson O, et al. Signal amplification and transduction in phytochrome photosensors[J]. Nature, 2014, 509: 245-248. doi: 10.1038/nature13310

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