PLFA analysis of soil microorganism under <i>Robinia pseudoacacia</i> and <i>Pinus tabuliformis</i> plantation in loess area

Wei Anqi; Wei Tianxing; Liu Haiyan; Wang Sha

doi:10.13332/j.1000-1522.20180287

Journal of Beijing Forestry University > 2019 > 41(4): 88-98. > DOI: 10.13332/j.1000-1522.20180287

Wei Anqi, Wei Tianxing, Liu Haiyan, Wang Sha. PLFA analysis of soil microorganism under Robinia pseudoacacia and Pinus tabuliformis plantation in loess area[J]. Journal of Beijing Forestry University, 2019, 41(4): 88-98. DOI: 10.13332/j.1000-1522.20180287

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PLFA analysis of soil microorganism under Robinia pseudoacacia and Pinus tabuliformis plantation in loess area

1.
School of Soil and Water Conservation, Jixian Forest Ecosystem Observation and Experiment Station of China National Ecosystem Research Network, Key Laboratory of State Forestry Administration on Soil and Water Conservation, Engineering Research Center of Forestry Ecological Engineering of Ministry of Education, Beijing Forestry University, Beijing 100083, China
2.
Fengtai Experimental School Affiliated to Minzu University of China, Beijing 100071, China

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Received Date: September 07, 2018
Revised Date: November 15, 2018
Available Online: April 29, 2019
Published Date: March 31, 2019

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Abstract

Abstract

Objective This paper aims to investigate the effects of artificial vegetation restoration on soil microbial community composition in the loess hilly-gully region, and provide data reference for regional vegetation restoration evaluation.
Method Three typical vegetation types, including Robinia pseudoacacia, Pinus tabuliformis plantation and grassland, were chosen in Jixian County, Shanxi Province of northern China. Soil samples from depth of 0−20 cm and 20−40 cm were collected respectively, and soil microbial community composition was evaluated by phospholipid fatty acid (PLFA) analysis (MIDI-Sherlock system). Based on the PLFA analysis, we studied the differences between soil microbial PLFA contents and soil microbial community after restoration, and further explored the effect of soil chemical properties on soil microbial community structure.
Result Results showed that total PLFAs, biomasses of bacteria, actinomycetes, gram-positive bacteria, arbuscular mycorrhizal fungi and diversity indicators (Shannon-Wiener, Simpson and Pielou index) followed the order of Robinia pseudoacacia > Pinus tabuliformis > grassland (P < 0.05). Meanwhile, the ratio of Saturated fatty acids to Monounsaturated fatty acids (SAT/MONO) in the two plantations was significantly lower than grassland (P < 0.05), which implied that environmental stress on soil microorganisms had reduced. Principal component analysis indicated that soil microbial community structure differed among various vegetation types and soil depths, and that the difference reached a significant level in topsoil (P < 0.05). Redundancy analysis and Pearson correlation analysis showed that soil total nitrogen and pH were the main factors leading to the changes of soil microbial community structure.
Conclusion The restoration of Robinia pseudoacacia, Pinus tabuliformis impacted the diversity of soil microbial community structure significantly, and both typical plantations had positive effect on soil ecosystem restoration. Our findings suggest that Robinia pseudoacacia was more conducive to the improvement of soil microbial ecological environment than Pinus tabuliformis.
- vegetation restoration,
- soil microorganism,
- phospholipid fatty acid (PLFA),
- community structure,
- diversity

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References (44)

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PLFA analysis of soil microorganism under Robinia pseudoacacia and Pinus tabuliformis plantation in loess area