QTL epistasis effect analysis of seedling growth-related traits in <i>Populus euphratica</i>

Wang Ping; Wang Dongyang; Wang Jing; Jiang Libo; Wu Rongling

doi:10.13332/j.1000-1522.20180332

Journal of Beijing Forestry University > 2018 > 40(12): 49-59. > DOI: 10.13332/j.1000-1522.20180332

Wang Ping, Wang Dongyang, Wang Jing, Jiang Libo, Wu Rongling. QTL epistasis effect analysis of seedling growth-related traits in Populus euphratica[J]. Journal of Beijing Forestry University, 2018, 40(12): 49-59. DOI: 10.13332/j.1000-1522.20180332

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QTL epistasis effect analysis of seedling growth-related traits in Populus euphratica

Center of Computational Biology, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China

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Received Date: October 16, 2018
Revised Date: November 06, 2018
Published Date: November 30, 2018

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Abstract

Abstract

ObjectiveSeedling stage plays a vital role in plant growth and development. Up to date there are no reports about genetic mechanism of epistasis interaction during seedling growth in Populus euphratica.
MethodIn this study, a full-sib F₁ population including 408 individuals was derived from Populus euphratica × Populus euphratica. Dynamic growth data of four phenotypes were measured, including the taproot length, shoot length, total lateral root length, and number of lateral roots. Based on a high-density genetic linkage map, 2HIGWAS was employed to identify QTL-QTL interactions associated with four phenotypes.
ResultFinally, a total of 83 pairs of QTL-QTL interactions were detected, including 83 SNPs. The taproot length, shoot length, total lateral root length and number of lateral roots found 24 pairs, 20 pairs, 24 pairs and 15 pairs epistatic interactions, respectively, and the epistatic interactions of main root length, stem height and lateral root length were distributed in linkage groups 1, 19, and 17, respectively. In addition, we conducted functional annotations for the SNPs with significant QTL-QTL epistatic interactions, and 19 QTLs were annotated with candidate genes.
ConclusionInteractions associated with total lateral root length had higher heritability, and most of the SNPs were located in linkage group 17, which might be important candidate gene regions. This work may provide guidance for molecular marker-assisted breeding of Populus euphratica and other forest trees.
- QTL,
- epistasis effect,
- 2HIGWAS,
- seedling growth,
- Populus euphratica

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References

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QTL epistasis effect analysis of seedling growth-related traits in Populus euphratica