Meiotic chromosome behavior of pollen mother cells and pollen variation in triploid hybrid between section Tacamahaca and sect. Aigeiros of <i>Populus</i>

Li Daili; Shang Jing; Tian Ju; Song Lianjun; Liu Chunhe; Li Yingchun; Kang Xiangyang; Wang Jun

doi:10.13332/j.1000-1522.20190099

Journal of Beijing Forestry University > 2019 > 41(7): 75-82. > DOI: 10.13332/j.1000-1522.20190099

Li Daili, Shang Jing, Tian Ju, Song Lianjun, Liu Chunhe, Li Yingchun, Kang Xiangyang, Wang Jun. Meiotic chromosome behavior of pollen mother cells and pollen variation in triploid hybrid between section Tacamahaca and sect. Aigeiros of Populus[J]. Journal of Beijing Forestry University, 2019, 41(7): 75-82. DOI: 10.13332/j.1000-1522.20190099

Citation:

PDF (734 KB)

Meiotic chromosome behavior of pollen mother cells and pollen variation in triploid hybrid between section Tacamahaca and sect. Aigeiros of Populus

1.
National Engineering Laboratory in Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
2.
Beijing Huangfa Nursery, Beijing 102601, China
3.
Breeding and Propagation Base for Tree Varieties in Weixian County, Weixian 054700, Hebei, China

More Information

Received Date: February 27, 2019
Revised Date: May 03, 2019
Available Online: June 30, 2019
Published Date: June 30, 2019

Graphical Abstract

Abstract

Abstract

ObjectiveRecently, techniques for induction of triploid hybrids between section Tacamahaca and sect. Aigeiros of Populus have been developed and a number of triploid germplasms were produced. However, reproductive developmental characteristics on these triploids are still in lack, which restrains their potential in sexual utilization. This study analyzed meiotic feature of pollen mother cells (PMCs) and pattern of gametic variation in a triploid hybrid, to lay a foundation for chromosomal engineering breeding of Populus using triploids as intermediate materials.
MethodIn this study, meiotic chromosomal behaviors of PMCs and pollen morphological variation between a male triploid hybrid WT-21, which derived from hybridization of induced 2n female gametes of P. pseudo-simonii × P. nigra ‘Zheyin3#’ and P. × beijingensis, and a male diploid hybrid WD-2 from the same combination were compared by the squashed technique with aceto-carmine.
Result(1) Whether for WT-21 or for WD-2, we observed the kinds of meiotic chromosomal behaviors, such as precocious chromosome migration, lagging chromosomes and micronuclei, reflecting high heterogeneity between parental genomes. (2) Misorientation of spindles during the second meiotic division, including parallel spindles, fused spindles and tripolar spindles, were found both in WT-21 and WD-2 and premature cytokinesis was produced in WT-21, which resulted in formation of dyads and triads in meiotic products. (3) In WT-21, 44.55% pollen grains were shrunken, which was higher than that of WD-2. Compared with WD-2, diameter of spherical pollen grains in WT-21 was significantly bigger than that of WD-2. From pollen diameter distribution, we could presume that the WT-21 could produce a small number of unreduced pollen grains. Pollen germination test showed that the germination rate of WT-21 was (1.08 ± 0.44)%, which was significantly lower than that of the WD-2 (28.67% ± 2.04%).
ConclusionAffected by both ploidy effect and heterozygosity, there are complex meiotic chromosomal behaviors during PMC meiosis in triploid hybrids, which would influence gametic development. Pollination with pollen of triploids would produce aneuploid and tetraploid offspings, laying the foundation for chromosome manipulation of Populus.
- Populus spp.,
- allotriploid,
- meiosis,
- pollen

FullText(HTML)

References (24)

References

[1]	Köhler C, Scheid O M, Erilova A. The impact of the triploid block on the origin and evolution of polyploid plants[J]. Trends in Genetics, 2010, 26(3): 142−148. doi: 10.1016/j.tig.2009.12.006
[2]	Johnsson H. Cytological studies of diploid and triploid Populus tremula and of crosses between them[J]. Hereditas, 1940, 26(3−4): 321−352.
[3]	Winton L, Einspahr D W. Tetraploid aspen production using unreduced triploid pollen[J]. Forest Science, 1970, 16(2): 180−182.
[4]	Harder M L, Verhagen S, Winton L, et al. Tetraploid aspen production using unreduced pollen from triploid males[J]. Forest Science, 1976, 22(3): 329−330.
[5]	康向阳, 毛建丰. 三倍体毛白杨配子育性及其子代形态变异研究[J]. 北京林业大学学报, 2001, 23(4):20−23. Kang X Y, Mao J F. Gamete fertility and morphological variations in offspring of triploid clones Populus tomentosa[J]. Journal of Beijing Forestry University, 2001, 23(4): 20−23.
[6]	Wang J, Huo B, Liu W, et al. Abnormal meiosis in an intersectional allotriploid of Populus and segregation of ploidy levels in 2x × 3x progeny[J/OL]. PLoS One, 2017, 12(7): e0181767 [2018−12−08]. https://doi.org/10.1371/journal.pone.0181767.
[7]	赵天锡, 陈章水. 中国杨树集约栽培[M]. 北京: 中国科学技术出版社, 1994. Zhao T X, Chen Z S. Intensive poplar cultivation in China[M]. Beijing: China Science & Technology Press, 1994.
[8]	Wang J, Kang X Y, Li D L, et al. Induction of diploid eggs with colchicine during embryo sac development in Populus[J]. Silvae Genetica, 2010, 59(1): 40−48.
[9]	Wang J, Li D L, Kang X Y. Induction of unreduced megaspores with high temperature during megasporogenesis in Populus[J]. Annals of Forest Science, 2012, 69(1): 59−67. doi: 10.1007/s13595-011-0152-5
[10]	Tian J, Wang J H, Dong L, et al. Pollen variation as a response to hybridisation in Populus L. section Aigeiros Duby[J]. Euphytica, 2015, 206(2): 433−443. doi: 10.1007/s10681-015-1507-z
[11]	R Development Core Team. R: a language and environment for statistical computing[M]. Vienna: Austria R Foundation for Statistical Computing, 2007.
[12]	Wang J, Kang X Y, Zhu Q. Variation in pollen formation and its cytological mechanism in an allotriploid white poplar[J]. Tree Genetics & Genomes, 2010, 6(2): 281−290.
[13]	Zhang Z H, Kang X Y, Zhang P D, et al. Incidence and molecular markers of 2n pollen in Populus tomentosa Carr.[J]. Euphytica, 2007, 154(1−2): 145−152. doi: 10.1007/s10681-006-9280-7
[14]	Zhang J F, Wei Z Z, Li D, et al. Using SSR markers to study the mechanism of 2n pollen formation in Populus × euramericana (Dode) Guinier and P. × popularis[J/OL]. Annals of Forest Science, 2009, 66(5): 506 [2018−12−08]. https://www.afs-journal.org/articles/forest/pdf/2009/05/f08222.pdf.
[15]	Mok D W S, Peloquin S J. Three mechanisms of 2n pollen formation in diploid potatoes[J]. Canadian Journal of Genetics and Cytology, 1975, 17(2): 217−225. doi: 10.1139/g75-029
[16]	Bretagnolle F, Thompson J D. Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of autopolyploid plants[J]. New Phytologist, 1995, 129(1): 1−22. doi: 10.1111/nph.1995.129.issue-1
[17]	Ramanna M S. A re-examination of the mechanisms of 2n gametes formation in potato and its implications for breeding[J]. Euphytica, 1979, 28(3): 537−561. doi: 10.1007/BF00038921
[18]	Vorsa N, Bingham E T. Cytology of 2n pollen formation in diploid alfalfa, Medicago sativa[J]. Canadian Journal of Genetics and Cytology, 1979, 21: 525−530. doi: 10.1139/g79-057
[19]	Becerra Lopez-Lavalle L A, Orjeda G. Occurrence and cytological mechanism of 2n pollen formation in a tetraploid accession of Ipomoea batatas (sweet potato)[J]. Journal of Heredity, 2002, 93(3): 185−192. doi: 10.1093/jhered/93.3.185
[20]	Carputo D, Cardi T, Frusciante L, et al. Male fertility and cytology of triploid hybrids between tetraploid Solanum commersonii (2n−4x−48, 2EBN) and Phureja-Tuberosum haploid hybrids (2n−2x−24, 2EBN)[J]. Euphytica, 1995, 83(2): 123−129. doi: 10.1007/BF01678039
[21]	Zhang Z, Kang X. Cytological characteristics of numerically unreduced pollen production in Populus tomentosa Carr.[J]. Euphytica, 2010, 173(2): 151−159. doi: 10.1007/s10681-009-0051-0
[22]	De Storme N, Geelen D. Sexual polyploidization in plants-cytological mechanisms and molecular regulation[J]. New Phytologist, 2013, 198(3): 670−684. doi: 10.1111/nph.12184
[23]	Risso-Pascotto C, Pagliarini M S, Borges do Valle C, et al. Asynchronous meiotic rhythm as the cause of selective chromosome elimination in an interspecific Brachiaria hybrid[J]. Plant Cell Reports, 2004, 22(12): 945−950.
[24]	Ramsey J, Schemske D W. Pathways, mechanisms, and rates of polyploid formation in flowering plants[J]. Annual Review of Ecology and Systematics, 1998, 29(1): 467−501. doi: 10.1146/annurev.ecolsys.29.1.467

Cited By

Cited by

Periodical cited type(12)

1.	杨春梅，李昱成，田心池，吴方镜，魏明，吴哲. 基于筛分配比的超薄纤维板物理力学性能研究. 林业机械与木工设备. 2024(01): 20-24 .
2.	张镭，邹淼，唐启恒，郭文静. 热压时间和温度对酚醛树脂型超薄高密度纤维板性能的影响. 木材科学与技术. 2023(01): 68-73 .
3.	高黎，常亮，郭文静，范丽颖，涂松，袁旻. 轻质纤维板发展与应用探讨. 中国人造板. 2023(06): 8-11 .
4.	史振丁，贾耀芳，郭勇德. 基于不同水刺水针板缠结性能的研究. 化纤与纺织技术. 2023(05): 20-22 .
5.	陈秀兰，杜官本，范恩庆，杨龙. 工艺参数对脲基超支化聚合物改性效果的影响. 林产工业. 2022(04): 20-24 .
6.	龙慈明，覃掌吉，黎耀健，卿彦，李新功，吴义强，刘明. 脲醛树脂/异氰酸酯胶粘剂组合施胶对纤维板性能的影响. 中国胶粘剂. 2021(02): 8-13 .
7.	黄伟雄，韦盛永，陆锋，冯炳健，李立群，徐健峰. 中密度纤维板的密度稳定性分析——以广西三威林产岑溪市人造板有限公司为例. 林业科技通讯. 2021(04): 63-66 .
8.	孙萍，张健，刘红光，李黎，罗斌. UF/PMDI与PMDI胶黏剂制备轻质刨花板性能对比研究. 林产工业. 2021(06): 7-12 .
9.	伍艳梅，卢胜高，赵丽媛，唐召群，杨娜. 中密度纤维板剖面密度特征参数与主要物理力学性能相关性. 木材科学与技术. 2021(05): 31-37 .
10.	张成旭，花军，陈光伟. 纤维目数对纤维板力学性能和成本的影响研究. 林产工业. 2020(03): 19-24 .
11.	马玉峰，龚轩昂，王春鹏. 木材胶黏剂研究进展. 林产化学与工业. 2020(02): 1-15 .
12.	林国利，覃掌吉，魏云和，安超，杨守禄，刘明. 工艺因子对MDI制备无醛纤维板性能的影响. 林产工业. 2020(08): 25-28 .

Other cited types(5)

Get Citation

PDF

XML

Article views (1916) PDF downloads (49) Cited by(17)

Turn off MathJax

Article Contents

Abstract

References

Meiotic chromosome behavior of pollen mother cells and pollen variation in triploid hybrid between section Tacamahaca and sect. Aigeiros of Populus

Abstract

References

Cited by

Periodical cited type(12)

Other cited types(5)

Catalog

Export File

Citation

Format

Content