Meiotic chromosome behavior of pollen mother cells and pollen variation in triploid hybrid between section Tacamahaca and sect. Aigeiros of <i>Populus</i>

Li Daili; Shang Jing; Tian Ju; Song Lianjun; Liu Chunhe; Li Yingchun; Kang Xiangyang; Wang Jun

doi:10.13332/j.1000-1522.20190099

Journal of Beijing Forestry University > 2019 > 41(7): 75-82. > DOI: 10.13332/j.1000-1522.20190099

Li Daili, Shang Jing, Tian Ju, Song Lianjun, Liu Chunhe, Li Yingchun, Kang Xiangyang, Wang Jun. Meiotic chromosome behavior of pollen mother cells and pollen variation in triploid hybrid between section Tacamahaca and sect. Aigeiros of Populus[J]. Journal of Beijing Forestry University, 2019, 41(7): 75-82. DOI: 10.13332/j.1000-1522.20190099

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Meiotic chromosome behavior of pollen mother cells and pollen variation in triploid hybrid between section Tacamahaca and sect. Aigeiros of Populus

1.
National Engineering Laboratory in Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
2.
Beijing Huangfa Nursery, Beijing 102601, China
3.
Breeding and Propagation Base for Tree Varieties in Weixian County, Weixian 054700, Hebei, China

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Received Date: February 27, 2019
Revised Date: May 03, 2019
Available Online: June 30, 2019
Published Date: June 30, 2019

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Abstract

Abstract

ObjectiveRecently, techniques for induction of triploid hybrids between section Tacamahaca and sect. Aigeiros of Populus have been developed and a number of triploid germplasms were produced. However, reproductive developmental characteristics on these triploids are still in lack, which restrains their potential in sexual utilization. This study analyzed meiotic feature of pollen mother cells (PMCs) and pattern of gametic variation in a triploid hybrid, to lay a foundation for chromosomal engineering breeding of Populus using triploids as intermediate materials.
MethodIn this study, meiotic chromosomal behaviors of PMCs and pollen morphological variation between a male triploid hybrid WT-21, which derived from hybridization of induced 2n female gametes of P. pseudo-simonii × P. nigra ‘Zheyin3#’ and P. × beijingensis, and a male diploid hybrid WD-2 from the same combination were compared by the squashed technique with aceto-carmine.
Result(1) Whether for WT-21 or for WD-2, we observed the kinds of meiotic chromosomal behaviors, such as precocious chromosome migration, lagging chromosomes and micronuclei, reflecting high heterogeneity between parental genomes. (2) Misorientation of spindles during the second meiotic division, including parallel spindles, fused spindles and tripolar spindles, were found both in WT-21 and WD-2 and premature cytokinesis was produced in WT-21, which resulted in formation of dyads and triads in meiotic products. (3) In WT-21, 44.55% pollen grains were shrunken, which was higher than that of WD-2. Compared with WD-2, diameter of spherical pollen grains in WT-21 was significantly bigger than that of WD-2. From pollen diameter distribution, we could presume that the WT-21 could produce a small number of unreduced pollen grains. Pollen germination test showed that the germination rate of WT-21 was (1.08 ± 0.44)%, which was significantly lower than that of the WD-2 (28.67% ± 2.04%).
ConclusionAffected by both ploidy effect and heterozygosity, there are complex meiotic chromosomal behaviors during PMC meiosis in triploid hybrids, which would influence gametic development. Pollination with pollen of triploids would produce aneuploid and tetraploid offspings, laying the foundation for chromosome manipulation of Populus.
- Populus spp.,
- allotriploid,
- meiosis,
- pollen

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References

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Meiotic chromosome behavior of pollen mother cells and pollen variation in triploid hybrid between section Tacamahaca and sect. Aigeiros of Populus