Pollen vigor and detection of exogenous genes of litter for transgenic triploid <i>Populus tomentosa</i>

Lü Wei; Sun Yuhan; Zhang Huaxin; Yang Qingshan; Li Juan; Chen Shouyi; Zhang Wanke; Li Yun

doi:10.13332/j.1000-1522.20190105

Journal of Beijing Forestry University > 2019 > 41(7): 91-100. > DOI: 10.13332/j.1000-1522.20190105

Lü Wei, Sun Yuhan, Zhang Huaxin, Yang Qingshan, Li Juan, Chen Shouyi, Zhang Wanke, Li Yun. Pollen vigor and detection of exogenous genes of litter for transgenic triploid Populus tomentosa[J]. Journal of Beijing Forestry University, 2019, 41(7): 91-100. DOI: 10.13332/j.1000-1522.20190105

Citation:

PDF (1848 KB)

Pollen vigor and detection of exogenous genes of litter for transgenic triploid Populus tomentosa

1.
National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
2.
Chinese Academy of Forestry, Beijing 100091, China
3.
Shandong Academy of Forestry, Jinan 250014, Shandong, China
4.
Cangzhou Municipal Forestry Seeding and Cutting Management Center, Cangzhou 061000, Hebei, China
5.
Institute of Genetics and Developmental Biology of Chinese Academy of Sciences, Beijing 100023, China

More Information

Received Date: February 27, 2019
Revised Date: April 15, 2019
Available Online: July 02, 2019
Published Date: June 30, 2019

Graphical Abstract

Abstract

Abstract

ObjectiveLong-term and continuous research on the safety evaluation of transgenic trees is significant to the development of forest tree genetic improvement and the biosafety evaluation of transgenic trees.
MethodIn this study, the pollen vigor and hybrid seed setting rate of transgenic triploid Populus tomentosa with AhDREB1 gene were continuously observed in the field for 11 years and 13 years. In addition, the foreign genes in the transgenic triploid Populus tomentosa were detected under natural conditions, and 44 kanamycin-resistant bacteria were screened from the rhizosphere soil of transgenic triploid P. tomentosa, and the genomic DNA of bacteria was used for PCR detection.
ResultThe results of MTT and TTC staining showed that the transgenic triploid Populus tomentosa pollen had no vigor. The results of hybridization test again showed that the transgenic triploid Populus tomentosa pollen may not have the cultability and fertility, and the hybrid seed setting rate was zero. The litter (branches, stems and leaves) of the transgenic poplars was buried in the soil, falling on the surface and falling on the lawn, and the foreign genes were not detected after 3 months. The exogenous gene in the 13-year-old transgenic triploid Populus tomentosa litter had not been transferred horizontally to the rhizosphere soil culturable bacterial genome.
ConclusionThis study did not find that the transgenic triploid P. tomentosa, which was grown in the field for 13 years, had a significant impact on the surrounding ecological environment.
- transgenic safety,
- vertical gene transfer,
- horizontal gene transfer,
- litter decomposition

FullText(HTML)

References (35)

References

[1]	Zhu Z T, Zhang Z Y. The status and advances of genetic improvement of Populus tomentosa Carr.[J]. Journal of Beijing Forestry University (English Edition), 1997, 6(1): 1−7.
[2]	朱之悌, 林惠斌, 康向阳. 毛白杨异源三倍体B301等无性系选育的研究[J]. 林业科学, 1995, 31(6):499−505. Zhu Z T, Lin H B, Kang X Y. Study on breeding of clonal lines of heterologous triploid B301 from Populus tomentosa[J]. Scientia Silvae Sinicae, 1995, 31(6): 499−505.
[3]	高玉红. 毛白杨试管苗耐盐性分析及耐盐植株初选[D]. 北京: 北京林业大学, 2004. Gao Y H. Analysis of salt-tolerant characteristics and primary selection of its salt-tolerant variants from tube plants of triplod Populus tomentosa[D]. Beijing: Beijing Forestry University, 2004.
[4]	丁莉萍, 王宏芝, 魏建华. 杨树转基因研究进展及展望[J]. 林业科学研究, 2016, 29(1):124−132. doi: 10.3969/j.issn.1001-1498.2016.01.018 Ding L P, Wang H Z, Wei J H. Progress and prospect of research in transgenic poplar[J]. Forest Research, 2016, 29(1): 124−132. doi: 10.3969/j.issn.1001-1498.2016.01.018
[5]	胡建军, 杨敏生, 卢孟柱. 我国抗虫转基因杨树生态安全性研究进展[J]. 生物多样性, 2010, 18(4):336−345. Hu J J, Yang M S, Lu M Z. Advances in biosafety studies on transgenic insect-resistant poplars in China[J]. Biodiversity Science, 2010, 18(4): 336−345.
[6]	Andow D A, Zwahlen C. Assessing environmental risks of transgenic plants[J]. Ecology Letters, 2006, 9(2): 196−214. doi: 10.1111/j.1461-0248.2005.00846.x
[7]	顾立江. 转Bt基因毛白杨外源基因横向转移环境风险分析[D]. 保定: 河北农业大学, 2009. Gu L J. Analysis of translocation environmental risk of transgenic Bt gene [D]. Baoding: Agricultural University of Hebei Province, 2009.
[8]	陈兴玲, 胡建军, 崔建国. 转基因林木生态安全性评价研究现状[J]. 辽宁林业科技, 2007(2):50−52. doi: 10.3969/j.issn.1001-1714.2007.02.016 Chen X L, Hu J J, Cui J G. Research status of ecological safety evaluation of transgenic trees[J]. Journal of Liaoning Forestry Science & Technology, 2007(2): 50−52. doi: 10.3969/j.issn.1001-1714.2007.02.016
[9]	吴迪. 转抗虫基因小黑杨叶片凋落物对土壤生态系统的影响[D]. 哈尔滨: 东北林业大学, 2008. Wu D. Influence of transgenic Populus simonii × P. nigra leaves on soil ecosystem[D]. Harbin: Northeast Forestry University, 2008.
[10]	李学红, 程贯召, 高明刚, 等. 遗传物质的横向传递与转基因植物的安全性[J]. 生态学杂志, 2005, 24(10):1221−1225. doi: 10.3321/j.issn:1000-4890.2005.10.022 Li X H, Cheng G Z, Gao M G, et al. Horizontal transfer of hereditary material and security of transgenic plants[J]. Chinese Journal of Ecology, 2005, 24(10): 1221−1225. doi: 10.3321/j.issn:1000-4890.2005.10.022
[11]	吴元凤, 李刚, 冀国桢, 等. 土壤环境中转基因植物重组DNA持留与水平转移研究进展[J]. 生态学杂志, 2015, 34(3):878−884. Wu Y F, Li G, Ji G Z, et al. Research progress of persistence and horizontal gene transfer of recombinant DNA from genetically modified plants in soil environment[J]. Chinese Journal of Ecology, 2015, 34(3): 878−884.
[12]	Crecchio C, Ruggiero P, Curci M, et al. Binding of DNA from Bacillus subtilis on montmorillonite-humic acids-aluminum or iron hydroxypolymers: effects on transformation and protection against DNase[J]. Soil Science Society of America Journal, 2005, 69(3): 834−841. doi: 10.2136/sssaj2004.0166
[13]	Zhu B, Ma B L, Blackshaw R E. Development of real time PCR assays for detection and quantification of transgene DNA of a Bacillus thuringiensis (Bt) corn hybrid in soil samples[J]. Transgenic Research, 2010, 19(5): 765−774. doi: 10.1007/s11248-009-9353-1
[14]	孙超, 台秀国, 杨振亚, 等. 不同贮藏温度对4种杨树花粉生活力的影响[J]. 山东科学, 2015, 28(1):109−113. doi: 10.3976/j.issn.1002-4026.2015.01.018 Sun C, Tai X G, Yang Z Y, et al. Impact of different storage temperature on pollen viability of four kinds of poplars[J]. Shandong Science, 2015, 28(1): 109−113. doi: 10.3976/j.issn.1002-4026.2015.01.018
[15]	Parton E, Vervaeke I, Delen R, et al. Viability and storage of bromeliad pollen[J]. Euphytica, 2002, 125(2): 155−161. doi: 10.1023/A:1015884019619
[16]	杜克兵, 沈宝仙, 许林, 等. 不同贮藏条件下杨树花粉活力变化及隔年杂交授粉应用的可行性研究[J]. 华中农业大学学报, 2007, 26(3):385−389. doi: 10.3321/j.issn:1000-2421.2007.03.026 Du K B, Shen B X, Xu L, et al. Changes of viability of stored poplar pollens and its feasibility for cross breeding[J]. Journal of Huazhong Agricultural University, 2007, 26(3): 385−389. doi: 10.3321/j.issn:1000-2421.2007.03.026
[17]	左丹丹, 明军, 刘春, 等. 植物花粉生活力检测技术进展[J]. 安徽农业科学, 2007, 35(16):4742−4745. doi: 10.3969/j.issn.0517-6611.2007.16.015 Zuo D D, Ming J, Liu C, et al. Advance in technique of plant pollen viability[J]. Journal of Anhui Agricultural Sciences, 2007, 35(16): 4742−4745. doi: 10.3969/j.issn.0517-6611.2007.16.015
[18]	张超仪, 耿兴敏. 六种杜鹃花属植物花粉活力测定方法的比较研究[J]. 植物科学学报, 2012, 30(1):92−99. Zhang C Y, Geng X M. Comparative study on methods for testing pollen viability of the six species from genus Rhododendron[J]. Plant Science Journal, 2012, 30(1): 92−99.
[19]	吕威, 卢楠, 侯荣轩, 等. 12年生转基因毛白杨外源基因转移及其对土壤微生物的影响[J]. 东北林业大学学报, 2018, 46(9):3−8. Lü W, Lu N, Hou R X, et al. Exogenous gene transfer of 12-year old transgenic hybrid of populus [(Populus tomentosa × P. bolleana) × P. tomentosa] and its effect on soil microbial quantity[J]. Journal of Northeast Forestry University, 2018, 46(9): 3−8.
[20]	朱文旭, 丁昌俊, 张伟溪, 等. 8年生转基因库安托杨外源基因转移及对土壤微生物数量影响的检测[J]. 林业科学研究, 2017, 30(2):349−353. Zhu W X, Ding C J, Zhang W X, et al. Exogenous gene transformation of 8-year-old multi-gene transgenic Populus × euramericana ‘Guariento ’ and its influence on soil microbial quantity[J]. Forest Research, 2017, 30(2): 349−353.
[21]	康向阳, 刘志民, 李胜功. 论转基因林木的潜在生态风险性[J]. 应用生态学报, 2004, 15(7):1281−1284. doi: 10.3321/j.issn:1001-9332.2004.07.036 Kang X Y, Liu Z M, Li S G. Potential ecological risks of transgenic trees[J]. Chinese Journal of Applied Ecology, 2004, 15(7): 1281−1284. doi: 10.3321/j.issn:1001-9332.2004.07.036
[22]	梁艳丽. 林木转基因技术的生态风险及伦理责任规制[J]. 南京林业大学学报(人文社会科学版), 2016, 16(4):30−38. doi: 10.3969/j.issn.1671-1165.2016.04.003 Liang Y L. Ecological risk and regulations of ethical responsibility in GM tree technology[J]. Journal of Nanjing Forestry University (Humanities and Social Sciences Edition), 2016, 16(4): 30−38. doi: 10.3969/j.issn.1671-1165.2016.04.003
[23]	姜金仲, 李云, 薛诺稳, 等. 林木遗传转化及其潜在生态风险研究进展[J]. 西北林学院学报, 2012, 27(1):103−123. doi: 10.3969/j.issn.1001-7461.2012.01.21 Jiang J Z, Li Y, Xue N W, et al. Researches progress on forest transformation and related potential ecological risks[J]. Journal of Northwest Forestry University, 2012, 27(1): 103−123. doi: 10.3969/j.issn.1001-7461.2012.01.21
[24]	Devos Y, Aguilera J, Diveki Z, et al. EFSA’s scientific activities and achievements on the risk assessment of genetically modified organisms (GMOs) during its first decade of existence: looking back and ahead[J]. Transgenic Research, 2013, 23(1): 1−25.
[25]	路兴波, 孙红炜, 杨崇良, 等. 转基因玉米外源基因通过花粉漂移的频率和距离[J]. 生态学报, 2005, 25(9):2450−2453. doi: 10.3321/j.issn:1000-0933.2005.09.044 Lu X B, Sun H W, Yang C L, et al. Gene flow of transgenic corn to cultivated relatives in China[J]. Acta Ecologica Sinica, 2005, 25(9): 2450−2453. doi: 10.3321/j.issn:1000-0933.2005.09.044
[26]	宋小玲, 皇甫超河, 强胜. 抗草丁膦和抗草甘膦转基因油菜的抗性基因向野芥菜的流动[J]. 植物生态学报, 2007, 31(4):729−737. doi: 10.3321/j.issn:1005-264X.2007.04.024 Song X L, Huangpu C H, Qiang S. Gene flow from transgenic glufosinate or glyphosate-tolerant oilseed rape to wild rape[J]. Chinese Journal of Plant Ecology, 2007, 31(4): 729−737. doi: 10.3321/j.issn:1005-264X.2007.04.024
[27]	姜大刚, 梁奕涛, 黄靖, 等. 转基因水稻外源基因的漂移研究[J]. 生物技术通报, 2010, 39(6):95−99. Jiang D G, Liang Y T, Huang J, et al. Study on gene flow of transgenic rice[J]. Biotechnology Bulletin, 2010, 39(6): 95−99.
[28]	张宝红, 郭腾龙. 转基因棉花基因花粉散布频率及距离的研究[J]. 应用与环境生物学报, 2000, 6(1):39−42. doi: 10.3321/j.issn:1006-687X.2000.01.008 Zhang B H, Guo T L. Frequency and distance of pollen dispersal from transgenic cotton[J]. Chinese Journal of Applied and Environmental Biology, 2000, 6(1): 39−42. doi: 10.3321/j.issn:1006-687X.2000.01.008
[29]	Miki B, Mchugh S. Selectable marker genes in transgenic plants: applications, alternatives and biosafety[J]. Journal of Biotechnology, 2004, 107(3): 193−232. doi: 10.1016/j.jbiotec.2003.10.011
[30]	Weinert N, Meincke R, Schioter M, et al. Effects of genetically modified plants on soil microorganisms[M]. Hoboken: John Wiley & Sons Inc, 2010: 235−258.
[31]	Zuo L, Yang R, Zhen Z, et al. A 5-year field study showed no apparent effect of the Bt transgenic 741 poplar on the arthropod community and soil bacterial diversity[J]. Scientific Reports, 2018, 8(1): 1956. doi: 10.1038/s41598-018-20322-3
[32]	Saxena D, Flores S, Stutzky G. Bt toxin is released in root exudates from 12 transgenic corn hybrids representing three transformation events[J]. Soil Biology & Biochemistry, 2002, 34(1): 133−137.
[33]	Gebhard F, Smalla K. Monitoring field releases of genetically modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer[J]. Fems Microbiology Ecology, 1999, 28(3): 261−272. doi: 10.1111/fem.1999.28.issue-3
[34]	邓欣, 赵廷昌, 高必达. 转基因抗虫棉叶围卡那霉素抗性细菌种群动态及nptⅡ基因漂移研究[J]. 中国农业科学, 2007, 40(11):2488−2494. doi: 10.3321/j.issn:0578-1752.2007.11.013 Deng X, Zhao T C, Gao B D. Dynamics in kanamycin resistant bacterial population and shift of nptII gene in the phyllosphere of insect resistant transgenic cotton[J]. Scientia Agricultura Sinica, 2007, 40(11): 2488−2494. doi: 10.3321/j.issn:0578-1752.2007.11.013
[35]	杨凤霞, 毛大庆, 罗义, 等. 环境中抗生素抗性基因的水平传播扩散[J]. 应用生态学报, 2013, 24(10):2993−3002. Yang F X, Mao D Q, Luo Y, et al. Horizontal transfer of antibiotic resistance genes in the environment[J]. Chinese Journal of Applied Ecology, 2013, 24(10): 2993−3002.

Cited By

Get Citation

PDF

XML

Article views (1824) PDF downloads (36)

Turn off MathJax

Article Contents

Abstract

References

Pollen vigor and detection of exogenous genes of litter for transgenic triploid Populus tomentosa

Abstract

References

Catalog

Export File

Citation

Format

Content