• Scopus
  • Chinese Science Citation Database (CSCD)
  • A Guide to the Core Journal of China
  • CSTPCD
  • F5000 Frontrunner
  • RCCSE
Advanced search
PANG Hong-dong, XIANG Lin, ZHAO Kai-ge, LI Xiang, YANG Nan, CHEN Long-qing. Genetic transformation and functional characterization of Chimonanthus praecox SAMT gene in tobacco[J]. Journal of Beijing Forestry University, 2014, 36(5): 117-122. DOI: 10.13332/j.cnki.jbfu.2014.05.019
Citation: PANG Hong-dong, XIANG Lin, ZHAO Kai-ge, LI Xiang, YANG Nan, CHEN Long-qing. Genetic transformation and functional characterization of Chimonanthus praecox SAMT gene in tobacco[J]. Journal of Beijing Forestry University, 2014, 36(5): 117-122. DOI: 10.13332/j.cnki.jbfu.2014.05.019

Genetic transformation and functional characterization of Chimonanthus praecox SAMT gene in tobacco

More Information
  • Published Date: September 29, 2014
  • In order to investigate the biological function of Chimonanthus praecox SAMT gene, CpSAMT was introduced into tobacco through Agrobacterium tumefaciens mediated transformation. Among 16 independent transgenic lines obtained, 15 lines were positive, which was revealed by PCR and further confirmed by RT-PCR. Further analysis showed that transgenic plants were indistinguishable from untransformed controls in terms of plant size, leaf morphology, flower color, petal size and flowering time. In addition, HS-SPME-GC-MS was employed to analyse the components of flower flavor. We found that the content of benzyl alcohol, hexenol, linalool, caryophyllene and benzaldehyde in transgenic CpSAMT plants increased, while that of methyl salicylate and methyl benzoate was undetectable compared with non-transgenic controls.
  • Related Articles

    [1]Feng Xuejing, Ma Ling, Yang Shuang, Bo Wenhao, Chen Xuexun, Pang Xiaoming. Construction of genetic transformation system of ‘Jingzao 39’ callus[J]. Journal of Beijing Forestry University, 2024, 46(10): 74-80. DOI: 10.12171/j.1000-1522.20240055
    [2]Jiang Shuaifei, Cui Ying, Zhao Ruirui, Qi Shuaizheng, Kong Lisheng, Zhao Jian, Li Shanshan, Zhang Jinfeng. Agrobacterium tumefaciens-mediated transformation of hybrid sweetgum embryogenic callus[J]. Journal of Beijing Forestry University, 2021, 43(8): 9-17. DOI: 10.12171/j.1000-1522.20210032
    [3]Cheng Jiali, Ma Jiang, Xiao Aihua, Zhu Zhonglong, Sang Ziyang, Ma Lüyi. Extraction, antioxidant and antibacterial activities of essential oils from Magnolia wufengensis[J]. Journal of Beijing Forestry University, 2020, 42(2): 96-105. DOI: 10.12171/j.1000-1522.20190249
    [4]Deng Wenhong, Zhao Xinrui, Zhang Junqi, Guo Huihong. Determination of plant hormones in plant tissues by UPLC-MS/MS[J]. Journal of Beijing Forestry University, 2019, 41(8): 154-160. DOI: 10.13332/j.1000-1522.20190052
    [5]LI Ran, QI Qi, LI Yun, CHEN Xue-mei, GAI Ying. A method of HPLC-MS/MS to determine chlorogenic acid and other three kinds of active components in Eucommia ulmoids[J]. Journal of Beijing Forestry University, 2016, 38(6): 123-129. DOI: 10.13332/j.1000-1522.20160105
    [6]LUO Shu-qin, ZHANG Xiang, ZHANG Ming, ZHANG Li-ping. Chemical composition of extracts from Larix gmelinii bark extracted by SFE-CO2[J]. Journal of Beijing Forestry University, 2014, 36(5): 142-145. DOI: 10.13332/j.cnki.jbfu.2014.05.003
    [7]LI Yan, ZHAO De-gang. Ipt gene promoting shoot regeneration in genetic transformation of Eucommia ulmoides Oliv[J]. Journal of Beijing Forestry University, 2011, 33(6): 90-93.
    [8]LI Jin-ke, CHEN Hua-jun, CHEN Shao-liang. Quantitative analysis of jasmonic acids, indole-3-acetic acid and abscisic acid in plant tissues by GC-MS[J]. Journal of Beijing Forestry University, 2010, 32(5): 143-148.
    [9]YU Lai, AN Xin-min, CAO Guan-lin, CHEN Zhong, ZHANG Zhi-yi. Genetic transformation of Populus tomentosa Carr. with sterility construct of PtAP3[J]. Journal of Beijing Forestry University, 2010, 32(5): 15-20.
    [10]LI Hui, CHEN Xiao-yang, LI Yun, LI Wei, DING Xia. Optimization of antibiotic concentration in genetic transformation of Populus alba[J]. Journal of Beijing Forestry University, 2005, 27(5): 118-121.

Catalog

    Article views (5665) PDF downloads (61149) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return