PANG Hong-dong, XIANG Lin, ZHAO Kai-ge, LI Xiang, YANG Nan, CHEN Long-qing. Genetic transformation and functional characterization of Chimonanthus praecox SAMT gene in tobacco[J]. Journal of Beijing Forestry University, 2014, 36(5): 117-122. DOI: 10.13332/j.cnki.jbfu.2014.05.019
Citation:
PANG Hong-dong, XIANG Lin, ZHAO Kai-ge, LI Xiang, YANG Nan, CHEN Long-qing. Genetic transformation and functional characterization of Chimonanthus praecox SAMT gene in tobacco[J]. Journal of Beijing Forestry University, 2014, 36(5): 117-122. DOI: 10.13332/j.cnki.jbfu.2014.05.019
PANG Hong-dong, XIANG Lin, ZHAO Kai-ge, LI Xiang, YANG Nan, CHEN Long-qing. Genetic transformation and functional characterization of Chimonanthus praecox SAMT gene in tobacco[J]. Journal of Beijing Forestry University, 2014, 36(5): 117-122. DOI: 10.13332/j.cnki.jbfu.2014.05.019
Citation:
PANG Hong-dong, XIANG Lin, ZHAO Kai-ge, LI Xiang, YANG Nan, CHEN Long-qing. Genetic transformation and functional characterization of Chimonanthus praecox SAMT gene in tobacco[J]. Journal of Beijing Forestry University, 2014, 36(5): 117-122. DOI: 10.13332/j.cnki.jbfu.2014.05.019
1 Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 430070,P. R. China;
In order to investigate the biological function of Chimonanthus praecox SAMT gene, CpSAMT was
introduced into tobacco through Agrobacterium tumefaciens mediated transformation. Among 16
independent transgenic lines obtained, 15 lines were positive, which was revealed by PCR and further
confirmed by RT-PCR. Further analysis showed that transgenic plants were indistinguishable from
untransformed controls in terms of plant size, leaf morphology, flower color, petal size and flowering
time. In addition, HS-SPME-GC-MS was employed to analyse the components of flower flavor. We found
that the content of benzyl alcohol, hexenol, linalool, caryophyllene and benzaldehyde in transgenic
CpSAMT plants increased, while that of methyl salicylate and methyl benzoate was undetectable compared
with non-transgenic controls.
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