Effects of gibberellic acid and chilling treatments on seed germination and seedling growth of Paeonia rockii hybrids

YU Ling; ZHONG Yuan; WANG Ying; CHENG Fang-yun

doi:DOI:10.13332/j.1000-1522.20140240

Journal of Beijing Forestry University > 2015 > 37(4): 120-126. > DOI: DOI:10.13332/j.1000-1522.20140240

YU Ling, ZHONG Yuan, WANG Ying, CHENG Fang-yun. Effects of gibberellic acid and chilling treatments on seed germination and seedling growth of Paeonia rockii hybrids[J]. Journal of Beijing Forestry University, 2015, 37(4): 120-126. DOI: DOI:10.13332/j.1000-1522.20140240

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Effects of gibberellic acid and chilling treatments on seed germination and seedling growth of Paeonia rockii hybrids

College of Landscape Architecture,Beijing Forestry University,Beijing,100083,P. R. China

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Received Date: July 09, 2014
Revised Date: December 21, 2014
Published Date: April 29, 2015

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Abstract

Abstract

We explored the effects of gibberellic acid (GA3) in releasing seed hypocotyl dormancy of Paeonia rockii hybrids, and investigated the effects of root length, duration of the chilling treatment ((3±1) ℃) and GA3 concentration on the epicotyl dormancy breaking and seedling growth. Results showed that 100 mg/L GA3 treatment was the best to promote rooting. During the process of seed germination, interaction of root length and chilling treatment had a significant effect on the first sprouting time and germination rate. GA3 and chilling treatment could effectively promote the germination of seeds with radicle roots longer than 40 mm; on this basis, the chilling treatment for 14 d, combined respectively with 100, 200 mg/L GA3 or 500 mg/L GA3, was in favor of seed germination. GA3 and chilling treatment could also promote effectively the growth of seedlings with radicle roots longer than 40 mm, and the chilling treatment for 14 d, combined with 500 mg/L GA3, was optimum for seedling growth. We summarized the optimum procedure for seed germination as follows. First, the seeds should be soaked in 100 mg/L GA3 solution for 24 h and stratified in sand until roots grew up to 40 mm in length. Then they should be sowed in plug trays under the chilling treatment for 14 d. Finally, after being watered with 500 mg/L GA3 solution, they can grow up into seedlings under the greenhouse conditions.
- Paeonia rockii,
- chilling treatment,
- gibberellic acid (GA3) treatment,
- seed rooting,
- seed germination,
- seedling growth

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[1]	YUAN J H,CORNILLE A,GIRAUD T,et al. Independent domestications of cultivated tree peonies from different wild peony species[J]. Molecular Ecology,2014,23(1):82-95.
[1]	CHENG F Y,LI J J,CHEN D Z,et al. Chinese flare mudan[M]. Beijing: Chinese Forestry Publishing House ,2005:44-46.
[2]	成仿云,李嘉珏,陈德忠,等.中国紫斑牡丹[M].北京:中国林业出版社,2005:44-46.
[2]	CHENG F Y,CHEN D Z. Studies on the selection and breeding of new hybrids from blotched tree peony (Paeonia rockii cvs.) and the cultivars classification of tree peony[J]. Journal of Beijing Forestry University, 1998,20(2):27-32.
[3]	成仿云,陈德忠. 紫斑牡丹新品种选育及牡丹品种分类研究[J].北京林业大学学报,1998,20(2):27-32.
[3]	CHENG F Y,LI J J,CHEN D Z. The natural propagation characteristics of wild tree peony species in China[J]. Acta Horticulturae Sinica, 1997,24 (2):180-184.
[4]	JING X M,ZHENG G H,PEI Y L,et al. A study on the relationship between seed germination of wild Paeonia rockii and P. szechuanica and their endangerment [J]. Chinese Biodiversity,1995,3(2):84-87.
[4]	成仿云,李嘉珏,陈德忠.中国野生牡丹自然繁殖特性研究[J].园艺学报,1997,24 (2):180-184.
[5]	ZENG Y J,WANG Y R,ZHUANG G H,et al. Seed germination responses of Reaumuria soongorica and Zygophyllum xanthoxylum to drought stress and sowing depth[J]. Acta Ecologica Sinica,2004,24(8):1629-1634.
[5]	景新明,郑光华,裴颜龙,等.野生紫斑牡丹和四川牡丹种子萌发特性及与其致濒的关系[J].生物多样性,1995,3(2):84-87.
[6]	ROSS J D. Metabolic aspects of dormancy[M]∥MURRAY D R. Seed physiology Vol. 2:Germination and reserve mobilization. Sydney:Academic Press,1984:45-75.
[6]	LIU Z G,SHEN B,ZHANG Y. Morphological responses to temperature,drought stress and their interaction during seed germination of Platycodon grandiflorum[J]. Acta Ecologica Sinica,2013,33(8):2615-2622.
[7]	FU T T,CHENG H Y,SONG S Q. Advances in studies of seed dormancy [J]. Chinese Bulletin of Botany,2009,44(5): 629-641.
[7]	KOORNNEEF M,BENTSINK L,HILHORST H. Seed dormancy and germination[J]. Current Opinion in Plant Biology,2002,5 (1):33-36.
[8]	曾彦军,王彦荣,庄光辉,等.红砂和霸王种子萌发对干旱与播深条件的适应[J].生态学报,2004,24(8):1629-1634.
[8]	WANG W Q,CHENG H Y. Progress in seed dormancy and germination of Arabidopsis mutants[J]. Chinese Bulletin of Botany,2006,23(6):625-633.
[9]	刘自刚,沈冰,张雁.桔梗种子萌发对低温、干旱及互作胁迫的响应[J].生态学报,2013,33(8):2615-2622.
[9]	PAN R C,WANG X J,LI N H. Plant physiology [M]. 6th ed. Beijing:Higher Education Press, 2008:218.
[10]	CHEN Q L,WANG H L,WANG Z F,et al. Effects of cold stratification and exogenous gibberellic acid (GA3) on seed germination and contents of endogenous gibberellins (GAs) and abscisic acid (ABA) in Cistanche deserticola Y. C. Ma[J]. Plant Physiology Communications,2009,45 (3):270-272.
[10]	付婷婷,程红焱,宋松泉.种子休眠的研究进展[J].植物学报,2009,44(5):629-641.
[11]	王伟青,程红焱. 拟南芥突变体种子休眠与萌发的研究进展[J]. 植物学通报,2006,23(6):625-633.
[11]	LI J J. Chinese peony[M]. Beijing: Chinese Forestry Publishing House, 1999:1-2.
[12]	潘瑞炽,王小菁,李娘辉.植物生理学 [M]. 6版. 北京:高等教育出版社, 2008:218.
[12]	ZENG D X,YIN W L,ZHAO X Q,et al. Propagation of Chinese tree peony (Paeonia suffruticosa Andr.) [J]. Journal of Beijing Forestry University,2000,22 (3):90-95.
[13]	JING X M,ZHENG G H,HONG D Y. Characteristic of germination and storage of seed in cultural Paeonia suffruticosa[J]. Plant Physiology Communications,1995,31 (4): 268-270.
[13]	陈庆亮,王华磊,王志芬,等. 低温层积与外源GA3对肉苁蓉种子萌发及其内源GA和ABA含量的影响[J].植物生理学通讯,2009,45 (3):270-272.
[14]	李嘉珏.中国牡丹与芍药[M].北京:中国林业出版社,1999:1-2.
[14]	ZHENG X M,ZHOU R B,GU L P,et al. The properties of dormancy and germination of Paeonia suffruticosa[J]. Plant Physiology Communications,1995,31(4):260-262.
[15]	CHENG F Y,DU X J. Effects of chilling and gibberellic acid on the seed germination and seedling growth in Paeonia ostii ‘Feng Dan’ [J]. Acta Horticulturae Sinica,2008,35(4):553-558.
[15]	曾端香,尹伟伦,赵孝庆,等.牡丹繁殖技术[J].北京林业大学学报,2000,22 (3):90-95.
[16]	BARTON L V,CHANDLER C. Effects of gibberellic on seed germination of tree peony[J]. Contribs Boyce Thompson Inst,1957,19:201-215.
[16]	MA H,LI Z H,ZHANG Y L,et al. Release of seed dormancy of Paeonia ludlowii[J]. Scientia Silvae Sinicae,2012,48(9):62-67.
[17]	景新明,郑光华,洪德元.栽培牡丹的种子萌发和贮藏特性[J].植物生理学通讯,1995,31 (4):268-270.
[17]	LIU X X,ZHANG Y L,MA H,et al. Studies on seed dormancy release of Paeonia delavayi[J]. Seed,2013,32(2):9-12.
[18]	郑相穆,周阮宝,谷丽萍,等.凤丹种子的休眠和萌发特性[J].植物生理学通讯,1995,31 (4): 260-262.
[18]	LIN S M. Effect of different hormone and moist-chilling on seed germination and seedling growth of Fengdan[D].Nanjing: Nanjing Agricultural University,2007.
[19]	成仿云,杜秀娟.低温与赤霉素处理对‘凤丹’牡丹种子萌发和幼苗生长的影响[J].园艺学报,2008,35 (4):553-558.
[19]	QIAN X Y. Physiological mechanism of breaking seed dormancy and effect of seedling quality on Paeonia ostii by different temperature and gibberellic acid[D]. Nanjing: Nanjing Agricultural University,2009.
[20]	马宏,李正红,张艳丽,等.大花黄牡丹种子休眠的解除[J].林业科学,2012,48(9):62-67.
[20]	GAO S P,LIU T,LIU G X,et al. The effect of GA3 on seed germination and seedling growth of Paeonia suffruticosa[J]. Seed,2009,28(4) :49-50,53.
[21]	刘秀贤,张艳丽,马宏,等.滇牡丹种子休眠解除效应研究[J].种子,2013,32(2):9-12.
[21]	LOU F F,ZHAO L S,LI Z Y. Preliminary study on seed dormancy and germination characteristics of Paeonia lutea wild population on West Mount,Kunming[J]. Journal of Southwest Forestry College, 2007,27(5):34-37.
[22]	林松明.激素与低温处理对凤丹种子萌发及幼苗生长的影响[D].南京:南京农业大学,2007.
[23]	钱仙云.温度与GA3解除凤丹种子休眠的生理代谢变化[D].南京:南京农业大学,2009.
[24]	高水平,刘涛,刘改秀,等. GA3对牡丹种子发芽及幼苗生长的影响[J].种子,2009,28(4) :49-50,53.
[25]	JING X M,ZHENG G H. The characteristics of seed germination and dormancy of four wild species of tree peonies and their bearing on endangerment[J]. Acta Phytophysiologica Sinica,1999,25:214-221.
[26]	娄方芳,赵林森,李宗艳.昆明西山野生黄牡丹种子休眠与萌发特性初步研究[J].西南林学院学报,2007,27(5):34-37.

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Effects of gibberellic acid and chilling treatments on seed germination and seedling growth of Paeonia rockii hybrids

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