Establishment of shoot regeneration system from leaves of Dendranthema×grandiflora

The effects of different factors,such as genotype,seedling age, position of leaves on seedling,Vc,charcoal,AgNO₃ and different hormone combinations, on shoot regeneration from leaves of seven different Dendranthema×grandiflora cultivars were studied.Results indicate that the genotype is an important factor in shoot regeneration. The infant leaves from seedlings with an age of 15--35d have high shoot regeneration.High concentrations of NAA accelerate shoot regeneration of Dendranthema×grandiflora ‘Ziyan'.No positive effect of AgNO₃ on shoot regeneration is observed.The optimum medium for ‘Ziyan' is MS+6-BA 1mg/L+NAA 0.5mg/L and MS+6-BA 2mg/L+NAA 1mg/L.MS+6-BA 2mg/L+NAA 0.2mg/L and MS+6BA 2 mg/L+NAA 0.1 mg/L is suitable for‘L12M57’.