MA Qing-guo, WANG Hua, PAN Gang, PEI Dong. FISH-AFLP analysis of walnut advanced trees in Tibet, southwestern China.[J]. Journal of Beijing Forestry University, 2011, 33(6): 86-89.
Citation:
MA Qing-guo, WANG Hua, PAN Gang, PEI Dong. FISH-AFLP analysis of walnut advanced trees in Tibet, southwestern China.[J]. Journal of Beijing Forestry University, 2011, 33(6): 86-89.
MA Qing-guo, WANG Hua, PAN Gang, PEI Dong. FISH-AFLP analysis of walnut advanced trees in Tibet, southwestern China.[J]. Journal of Beijing Forestry University, 2011, 33(6): 86-89.
Citation:
MA Qing-guo, WANG Hua, PAN Gang, PEI Dong. FISH-AFLP analysis of walnut advanced trees in Tibet, southwestern China.[J]. Journal of Beijing Forestry University, 2011, 33(6): 86-89.
1 Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing, 100091, P. R. China; 2 College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, P. R. China; 3 Research Institute of Plateau Ecology, Tibet Agriculture and Animal Husbandry College, Linzhi, 860000, P. R. China.
In order to deeply understand the genetic background and the genetic diversity of Tibet walnut germplasm resources, FISHAFLP was conducted using 9 EcoRⅠ+3/MseⅠ+3 primer combinations to analyse 11 walnut (Juglans regia L.) advanced trees from Tibet at genomic DNA level. Totally 1 103 AFLP fragments were obtained, in which 1 076 were polymorphic and the average percentage of polymorphic bands (ppb) was 97.67%. The value of effective number of alleles (Ne) ranged from 1.00 to 1.99 with an average of 1.48±0.04; Neis gene diversity (H) ranged from 0.00 to 0.49 with an average of 0.29±0.02 and Shannons information index (I) ranged from 0.00 to 0.69 with an average of 0.44±0.02The genetic diversity and sibship among walnut advanced trees in Tibet were investigated and their fingerprintings were established in the research.
DownLoad: