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芍药根茎解剖结构特征及生长轮分析

张建军 陈莉祺 李建光 孙苗 范永明 于晓南

张建军, 陈莉祺, 李建光, 孙苗, 范永明, 于晓南. 芍药根茎解剖结构特征及生长轮分析[J]. 北京林业大学学报, 2020, 42(5): 124-131. doi: 10.12171/j.1000-1522.20190096
引用本文: 张建军, 陈莉祺, 李建光, 孙苗, 范永明, 于晓南. 芍药根茎解剖结构特征及生长轮分析[J]. 北京林业大学学报, 2020, 42(5): 124-131. doi: 10.12171/j.1000-1522.20190096
Zhang Jianjun, Chen Liqi, Li Jianguang, Sun Miao, Fan Yongming, Yu Xiaonan. Anatomical structure characteristics and growth ring analysis of underground rhizome of herbaceous peony[J]. Journal of Beijing Forestry University, 2020, 42(5): 124-131. doi: 10.12171/j.1000-1522.20190096
Citation: Zhang Jianjun, Chen Liqi, Li Jianguang, Sun Miao, Fan Yongming, Yu Xiaonan. Anatomical structure characteristics and growth ring analysis of underground rhizome of herbaceous peony[J]. Journal of Beijing Forestry University, 2020, 42(5): 124-131. doi: 10.12171/j.1000-1522.20190096

芍药根茎解剖结构特征及生长轮分析

doi: 10.12171/j.1000-1522.20190096
基金项目: 国家自然科学基金项目(31400591)
详细信息
    作者简介:

    张建军,博士生。主要研究方向:芍药成花机理。Email:zhang727jianjun@126.com 地址:100083北京市海淀区清华东路35号北京林业大学园林学院

    责任作者:

    于晓南,博士,教授。主要研究方向:园林植物资源与育种。Email:yuxiaonan626@126.com 地址:同上

  • 中图分类号: S682.1+2

Anatomical structure characteristics and growth ring analysis of underground rhizome of herbaceous peony

  • 摘要: 目的观察分析不同芍药品种群品种的根茎结构发育特点,对芍药根茎进行解剖学研究,初步解析芍药根茎次生结构及生长轮特征,以期为通过根茎结构判断芍药株龄、优化无性繁殖和栽培管理技术,促进资源的开发和利用研究提供一定的理论依据。方法以芍药不同品种群的6个品种不同发育时期的地下根茎为研究对象,观察并分析其发育更新特征,应用石蜡切片和徒手切片技术,对不同品种、同一品种不同生长年限的根茎次生结构特征进行观察分析。结果不同芍药品种的地下根茎组织架构基本一致,即由根茎、着生于根茎上的根茎芽和根3部分组成,根茎每年有序更新,年龄分级特征明显;四倍体品种‘Cream Delight’根茎发育特征与其余5个品种差异明显;6个芍药品种根茎次生结构由周皮、皮层、次生韧皮部、形成层、次生木质部和中央髓组成。二倍体‘种生粉’、二倍体‘粉玉奴’、三倍体‘Coral Sunset’和四倍体‘Cream Delight’4个品种根茎次生木质部大小导管有规律地依次排列,口径较大的导管和周围的小导管聚集形成群团状,导管群分布较稀疏,两导管群之间的间隔明显。与四倍体‘Cream Delight’相比,三倍体‘Coral Sunset’的导管群分布较紧凑。伊藤杂种两个三倍体品种‘Prairie Charm’和‘Going Bananas’根茎的次生木质部大小导管分布较均匀,形成较连续的环带,并不聚集形成团块状。芍药根茎截面在脱水后维管组织呈白色或淡黄色,间断环状分布,中央髓部组织下凹,位于不同环的维管组织从髓部向皮层呈放射状排列。不同生长年限的根茎中次生木质部口径较大的导管及其周围的小导管聚集呈团块状,导管群切向断续有规律地依次排列成与形成层平行的环,形成清晰的生长轮,且生长轮的数目与其实际生长年龄一致。结论芍药不同品种地下根茎组织架构特征基本一致,且存在明显的龄级特征。二倍体及三倍体各品种根茎发育特征相似,而与四倍体不同。中国芍药及杂种芍药品种群品种根茎次生结构相似而与伊藤杂种差异明显,杂种芍药品种群内三倍体及四倍体品种根茎次生结构差异较大,芍药根茎生长轮次生结构特征与品种倍性无关。芍药根茎中的生长轮即为其年轮,且其数目能够反映芍药的实际生长年限。

     

  • 图  1  芍药根茎结构发育示意

    1YR:1龄生根茎;2YR:2龄生根茎;3YR:3龄生根茎;4YR:4龄生根茎;5YR:5龄生根茎;RB:根茎芽;St:茎;AR:不定根。1YR, 1 year old rhizome; 2YR, 2 years old rhizome; 3YR, 3 years old rhizome; 4YR, 4 years old rhizome; 5YR, 5 years old rhizome; RB, rhizome bud; St, stem; AR, adventitious root.

    Figure  1.  Structural characteristic of rhizome of herbaceous peony

    图  2  芍药不同品种地下根茎发育结构特征

    a、b. ‘珊瑚落日’根茎;c、d. ‘草原风情’根茎;RB:根茎芽;Rt:根;Rh:根茎;St:茎。a, b, the rhizome of‘Coral Sunset’; c, d, the rhizome of ‘Cream Delight’; RB, rhizome bud; Rt, root; Rh, rhizome; St, stem.

    Figure  2.  Structural characteristics of rhizomes of different cultivars of herbaceous peony

    图  3  不同品种根茎解剖结构

    a、b. ‘大富贵’根茎解剖结构;c、d. ‘粉玉奴’根茎解剖结构;e、f. ‘珊瑚落日’根茎解剖结构;g、h. ‘乳霜之愉’根茎解剖结构;i、j. ‘草原风情’根茎解剖结构;k、l. ‘抓狂的香蕉’根茎解剖结构;Pe:周皮;Sp:次生韧皮部;Vc:维管形成层;Sx:次生木质部;Pi:髓。标尺 = 1 000 μm。a, b, rhizome anatomy of ‘Dafugui’; c, d, rhizome anatomy of ‘Fenyunu’; e, f, rhizome anatomy of ‘Coral Sunset’; g, h, rhizome anatomy of ‘Cream Delight’; i, j, rhizome anatomy of ‘Prairie Charm’; k, l, rhizome anatomy of ‘Going Bananas’; Pe, periderm; Sp, secondary phloem; Vc, vascular cambium; Sx, secondary xylem; Pi, pith. Scale bar = 1 000 μm.

    Figure  3.  Anatomical structure of rhizomes of different cultivars of herbaceous peony

    图  4  芍药‘粉玉奴’根茎横切面结构

    a、b. 2龄生根茎;c、d. 6龄生根茎;Sx:次生木质部;Pi:髓。标尺 = 1 000 μm。a, b, 2 years old rhizome; c, d, 6 years old rhizome; Sx, secondary xylem; Pi, pith. Scale bar = 1 000 μm.

    Figure  4.  Cross section structure of rhizome of Paeonia lactiflora ‘Fenyunu’

    图  5  芍药‘粉玉奴’根茎横切面显微结构

    a. 2龄生根茎;b. 4龄生根茎;Vc:维管形成层;Pi:髓。标尺 = 1 000 μm。a, 2 years old rhizome; b, 4 years old rhizome; Vc, vascular cambium; Pi, pith. Scale bar = 1 000 μm.

    Figure  5.  Microstructure of the cross section of the rhizome of Paeonia lactiflora ‘Fenyunu’

    图  6  芍药根茎不同生长年限生长轮观察

    a. 1龄生根茎;b. 2龄生根茎;c. 3龄生根茎;d. 4龄生根茎;e. 5龄生根茎;f、g. 6龄生根茎;h、i. 7龄生根茎;①. 第1个生长轮;②. 第2个生长轮;③. 第3个生长轮;④. 第4个生长轮;⑤. 第5个生长轮;⑥. 第6个生长轮;⑦. 第7个生长轮。标尺 = 1 000 μm。a, 1 year old rhizome; b, 2 years old rhizome; c, 3 years old rhizome; d, 4 years old rhizome; e, 5 years old rhizome; f, g, 6 years old rhizome; h, i, 7 years old rhizome; ①, the first growth ring; ②, the second growth ring; ③, the third growth ring; ④, the fourth growth ring; ⑤, the fifth growth ring; ⑥, the sixth growth ring; ⑦, the seventh growth ring. Scale bar = 1 000 μm.

    Figure  6.  Observation on the growth rings of the rhizome of herbaceous peony under a stereomicroscope

    表  1  供试芍药品种信息

    Table  1.   Variety information of experimental materials

    编号 No.品种名称 Variety name品种群分类 Classification of cultivar groups倍性 Ploidy level
    1 ‘种生粉’ ‘Zhongshengfen’ 中国芍药品种群 Lactiflora group 2n = 2x = 10
    2 ‘粉玉奴’ ‘Fenyunu’ 中国芍药品种群 Lactiflora group 2n = 2x = 10
    3 ‘珊瑚落日’ ‘Coral Sunset’ 杂种芍药品种群 Hybrid group 2n = 3x = 15
    4 ‘乳霜之愉’ ‘Cream Delight’ 杂种芍药品种群 Hybrid group 2n = 4x = 20
    5 ‘草原风情’ ‘Prairie Charm’ 伊藤杂种品种群 Itoh hybrid group 2n = 3x = 15
    6 ‘抓狂的香蕉’ ‘Going Bananas’ 伊藤杂种品种群 Itoh hybrid group 2n = 3x = 15
    下载: 导出CSV
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  • 收稿日期:  2019-02-28
  • 修回日期:  2019-05-23
  • 网络出版日期:  2019-10-17
  • 刊出日期:  2020-07-01

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    /

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    返回