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    蓝莓种质资源表型多样性研究

    Phenotypic diversity analysis of blueberry germplasm resources

    • 摘要:
      目的以山东和吉林两地的蓝莓品种为研究对象,探究蓝莓种质资源表型性状遗传多样性,为种质资源保存和基因改良提供理论依据。
      方法对47个蓝莓品种表型性状进行测量,结合主成分分析、相关分析及聚类分析等方法,探讨蓝莓品种间的表型多样性。
      结果主成分分析表明,前4个主成分特征根值大于0.5的包含25个性状,累计贡献率达53.48%,占调查指标的67.57%。表型数量性状间呈极显著相关的有62对,显著相关的有40对。蓝莓品种种质资源表型性状变异丰富,质量性状与数量性状的香浓维纳多样性指数分布在0.32 ~ 1.36(平均0.87)和0.22 ~ 2.06(平均1.8)。其中质量性状15个,包含52个变异类型,数量性状22个,变异系数在11.39% ~ 46.98%之间。说明蓝莓表型数量性状相对于质量性状表现出更广泛的变异。对调查性状降维后,保留25个主要性状做Q型聚类分析,当欧式距离为17时,将47个蓝莓品种分为5个组群,各组群与栽培生境和栽培类群有一定相关性。
      结论本研究明确了吉林、山东两地的40个蓝莓品种表型多样性与亲缘关系,今后在培育蓝莓新品种选择亲本时,应着重考虑树冠型、灌丛高度、树冠长径、果粉厚度、果实萼片着生姿势、果实硬度、果实萼片长宽比、果实萼片类型、长势等指标。研究结果可为优良种质资源评价与筛选提供参考。

       

      Abstract:
      ObjectiveThe genetic diversity of phenotypic traits of blueberry germplasm resources was studied in Shandong and Jilin provinces to provide theoretical basis for the conservation and genetic improvement of the germplasm resources.
      MethodThe phenotypic traits of 47 blueberry varieties were measured, and the phenotypic diversity among blueberry varieties was discussed by principal component analysis, correlation analysis and cluster analysis.
      ResultPrincipal component analysis showed that the first four principal component characteristic root values greater than 0.5 contained 25 traits, with a cumulative contribution rate of 53.477%, accounting for 67.57% of the survey indicators. 62 pairs were significantly correlated with the number of phenotypic traits, 40 pairs were significantly correlated. Blueberry varieties, phenotypic trait variation of rich germplasm resources, quality traits and aromatic wiener diversity index distribution of quantitative traits ranged in 0.32−1.36 (average 0.87) and 0.22−2.06 (average 1.8), the quality characters of 15, contained 52 mutation types. There were 22 quantitative traits, and the coefficient of variation ranged from 11.39% to 46.98%. This indicated that the quantitative traits of blueberry phenotype showed more extensive variation than the qualitative traits.When the Euclidean distance was 17, 47 blueberry varieties were divided into 5 groups. Each group had a certain correlation with the cultivated habitat and cultivated group.
      ConclusionIn this study, we identified 40 blueberry varieties of Jilin Province and Shandong Province both phenotypic diversity and genetic relationship, the choice in cultivating blueberry new varieties, parents should focus on canopy, shrub height, canopy length to diameter, thickness of fruit powder, fruit sepals by posture, fruit hardness, fruit sepals length-width ratio, looks like the fruit sepals type, such as index, the results can provide reference for excellent germplasm resource evaluation and selection.

       

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