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    松壳黄酮提取工艺及其抗氧化活性

    Extraction technology of flavonoids from pine shell and its antioxidant activity

      摘要
    • 摘要:
        目的  红松是东北地区重要的经济型树种，其松壳中所含的化学成分具有多种生物活性，特别是黄酮含量极为丰富，是一类天然抗氧化剂，而松壳多被视为废弃物被送至垃圾场填埋或焚烧，导致资源的浪费。因此深入研究松壳黄酮对红松的开发利用具有现实指导意义。
        方法  以红松松壳为原料，采用响应面法优化超声波辅助低共熔溶剂提取松壳中黄酮类化合物的工艺，并对提取物的抗氧化活性进行了研究。
        结果  松壳黄酮最佳提取工艺参数为：DES含水量40%、超声功率200 W、提取时间30 min、提取温度为60 ℃、固液比(w/v)为1∶55，此条件下黄酮得率为（9.838 ± 0.211）%，较醇提法提高69%。低共熔溶剂黄酮提取物对DPPH•、ABTS+•及•OH清除率的IC50分别为（5.195 ± 0.039）μg/mL、（9.528 ± 0.132）μg/mL、（151.860 ± 0.238）μg/mL，较醇提黄酮分别降低40.98%、52.94%、11.59%。以HepG2细胞建立H2O2氧化损伤模型，黄酮浓度在0.1 ~ 0.5 mg/mL范围内，对HepG2受损细胞具有保护作用，存在显著的剂效关系，且低共熔溶剂所提黄酮的保护作用优于醇提黄酮。
        结论  采用绿色环保的低共熔溶剂可高效提取松壳黄酮，且提取物能够保持良好的抗氧化活性。

       

      Abstract:
        Objective  Pinus koraiensis is an important economic tree species in northeastern China. The chemical components in its pine shell have many biological activities, especially the content of flavonoids is very rich. It is a kind of natural antioxidant, and the pine shell is regarded as the waste, which is sent to the landfill or burned, resulting in the waste of resources. Therefore, in-depth study on flavonoids in pine shell has practical significance for the development and utilization of Korean pine.
        Method  Response surface methodology was used to optimize the extraction process of flavonoids from Korean pine shell by ultrasonic assisted deep eutectic solvents, and the antioxidant activity of the extract was studied.
        Result  The best extraction parameters of flavonoids were: the moisture content of DES was 40%, ultrasonic power was 200 W, extraction time was 30 min, extraction temperature was 60 ℃, solid liquid ratio (w/v) was 1∶55. Under these conditions, the yield of flavonoids was (9.838 ± 0.211)%, 69% higher than that of alcohol extraction. The IC50 of DPPH•, ABTS+• and •OH scavenging rate of flavone extracted by DES were(5.195 ± 0.039) μg/mL, (9.528 ± 0.132) μg/mL, (151.860 ± 0.238) μg/mL, respectively, which were 40.98%, 52.94% and 11.59% lower than those of flavone extracted by alcohol extraction. When the concentration of flavone was in the range of 0.1−0.5 mg/ml, it had a protective effect on HepG2 damaged cells, and the protective effect of flavone extracted by DES was better than that extracted by alcohol.
        Conclusion  The green and environment-friendly DES can be used to extract flavonoids from pine shell, and the extract can maintain good antioxidant activity.

       

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