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    曲凯, 国浩平, 王宝锐, 周文玲, 侯丽丽, 李琴, 李际红, 程甜甜. 基于SRAP分子标记的流苏树天然群体遗传多样性研究[J]. 北京林业大学学报, 2020, 42(12): 40-50. DOI: 10.12171/j.1000-1522.20200212
    引用本文: 曲凯, 国浩平, 王宝锐, 周文玲, 侯丽丽, 李琴, 李际红, 程甜甜. 基于SRAP分子标记的流苏树天然群体遗传多样性研究[J]. 北京林业大学学报, 2020, 42(12): 40-50. DOI: 10.12171/j.1000-1522.20200212
    Qu Kai, Guo Haoping, Wang Baorui, Zhou Wenling, Hou Lili, Li Qin, Li Jihong, Cheng Tiantian. Genetic diversity analysis of Chionanthus retusus natural population based on SRAP molecular markers[J]. Journal of Beijing Forestry University, 2020, 42(12): 40-50. DOI: 10.12171/j.1000-1522.20200212
    Citation: Qu Kai, Guo Haoping, Wang Baorui, Zhou Wenling, Hou Lili, Li Qin, Li Jihong, Cheng Tiantian. Genetic diversity analysis of Chionanthus retusus natural population based on SRAP molecular markers[J]. Journal of Beijing Forestry University, 2020, 42(12): 40-50. DOI: 10.12171/j.1000-1522.20200212

    基于SRAP分子标记的流苏树天然群体遗传多样性研究

    Genetic diversity analysis of Chionanthus retusus natural population based on SRAP molecular markers

    • 摘要:
        目的  揭示我国不同地区流苏树(Chionanthus retusus)天然群体的遗传多样性,更好地为合理保护和开发利用提供科学依据。
        方法  采用相关序列扩增多态性(SRAP)分子标记技术对不同地区的7个流苏树天然群体的62份样品进行了遗传多样性和群体遗传结构研究。
        结果  (1)7个流苏树天然群体具有较高的遗传多样性,8对SRAP引物共扩增出1 728条清晰条带,其中1 649条具有多态性,PPB(多态性条带比例)为95.43%;群体间的有效等位基因数为 1.213 7,Nei’s基因多样性指数为 0.153 7,Shannon’s信息多样性指数为0.268 0。(2)流苏树天然群体存在较高水平的种群内遗传变异和较低水平的群体间遗传变异(Gst = 0.133 6),7个流苏树天然群体间存在较高水平的基因交流(Nm = 3.243 7)。(3)流苏树群体间的遗传相似系数介于0.898 0 ~ 0.973 6之间,平均值为0.934 4,经Mantel检验(r = 0.288,P = 0.205)及群体间的聚类证明群体间的遗传距离与地理距离之间无明显相关性;62份流苏树初级种质聚类结果表明大部分种质表现为同一群体的多数个体聚在一起,部分种质存在不同群体间的个体聚在一起的现象,表现出群体间遗传变异相对稳定而种群内的遗传变异水平相对较高的特点,与基因多样性分析结果一致。
        结论  综合多因素分析推测,太行山地区可能是我国流苏树种质资源的主要产区。

       

      Abstract:
        Objective  This paper aims to reveal the genetic diversity of natural populations of Chionanthus retusus in different regions of China, and to provide a scientific basis for rational protection development and utilization.
        Method  The genetic diversity and population genetic structure of 62 samples from 7 Chionanthus retusus natural populations in different regions were studied using sequence related amplified polymorphism (SRAP) molecular marker technique.
        Result  Seven natural populations of Chionanthus retusus had higher genetic diversity, and 8 pairs of SRAP primers amplified a total of 1 728 clear bands, of which 1 649 were polymorphic, and the percentage of polymorphic bands (PPB) was 95.43%. The number of effective alleles between populations was 1.213 7, the diversity of Nei’s gene was 0.153 7, and the information diversity index of Shannon’s was 0.268 0. There were higher levels of intra-population genetic variation and lower levels of inter-population genetic variation among natural populations of Chionanthus retusus (Gst = 0.133 6), and higher levels of gene flow among seven natural populations of Chionanthus retusus (Nm = 3.243 7). The genetic similarity coefficient between Chionanthus retusus populations ranged from 0.898 0 to 0.973 6, with an average of 0.934 4. The Mantel test (r = 0.288, P = 0.205) and the clustering among populations proved that there was no significant correlation between genetic distance and geographical distance among populations. The clustering results of 62 primary germplasm showed that most of the germplasms were characterized by the fact that most individuals in the same population came together, and some germplasms had the phenomenon that individuals of different populations gathered together, showing that the genetic variation between populations was relatively stable and the level of genetic variation within the populations was relatively high, which was consistent with the results of genetic diversity analysis.
        Conclusion  Comprehensive multi-factor analysis speculated that Taihang Mountain area may be the main producing area of Chinese Chionanthus retusus germplasm resources.

       

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