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BpCCR1基因白桦生长适应性分析及优良株系选择

王楚 王阳 邹建军 彭儒胜 刘桂丰 姜静

王楚, 王阳, 邹建军, 彭儒胜, 刘桂丰, 姜静. 转BpCCR1基因白桦生长适应性分析及优良株系选择[J]. 北京林业大学学报, 2022, 44(7): 52-62. doi: 10.12171/j.1000-1522.20200264
引用本文: 王楚, 王阳, 邹建军, 彭儒胜, 刘桂丰, 姜静. 转BpCCR1基因白桦生长适应性分析及优良株系选择[J]. 北京林业大学学报, 2022, 44(7): 52-62. doi: 10.12171/j.1000-1522.20200264
Wang Chu, Wang Yang, Zou Jianjun, Peng Rusheng, Liu Guifeng, Jiang Jing. Growth adaptability analysis of BpCCR1 transgenic Betula platyphylla and selection of elite lines[J]. Journal of Beijing Forestry University, 2022, 44(7): 52-62. doi: 10.12171/j.1000-1522.20200264
Citation: Wang Chu, Wang Yang, Zou Jianjun, Peng Rusheng, Liu Guifeng, Jiang Jing. Growth adaptability analysis of BpCCR1 transgenic Betula platyphylla and selection of elite lines[J]. Journal of Beijing Forestry University, 2022, 44(7): 52-62. doi: 10.12171/j.1000-1522.20200264

BpCCR1基因白桦生长适应性分析及优良株系选择

doi: 10.12171/j.1000-1522.20200264
基金项目: 林木遗传育种国家重点实验室创新项目(2020A01)
详细信息
    作者简介:

    王楚。主要研究方向:林木遗传育种。Email:wangchu1226@163.com 地址:150040 黑龙江省哈尔滨市香坊区和兴路51号东北林业大学林木遗传育种国家重点实验室

    责任作者:

    姜静,教授,博士生导师。主要研究方向:林木遗传育种。Email:jiangjing1960@126.com 地址:同上

  • 中图分类号: S722.5

Growth adaptability analysis of BpCCR1 transgenic Betula platyphylla and selection of elite lines

  • 摘要:   目的  客观评估7年生转BpCCR1白桦在3个试验地点的生长适应性与稳定性,筛选优良转基因株系,为环境释放及生产性试验提供指导。  方法  以21个转BpCCR1白桦株系及1个野生型白桦株系(WT)为研究对象,测定其在帽儿山、石道河林场、生态实验林场3个试验地点的树高、胸径及材积生长,用AMMI模型分析参试株系与环境的互作效应、遗传稳定性和生长适应性。  结果  方差分析结果显示:树高生长在株系间、地点间以及株系与地点的交互作用上的差异达到极显著水平(P < 0.01)。以各试验点参试株系单株材积均值加上标准差为优良转基因株系选择标准,分别选择出各造林地点的最优株系。帽儿山试验点的入选优良株系是FC18、FC29和FC40,材积分别高于群体均值的72.99%、51.28%和64.39%;石道河林场的入选优良株系是FC7和FC33,材积分别高于群体均值的73.98%和81.51%;生态实验林场的入选优良株系是FC28和FC29,分别高于群体均值的69.89%和73.57%。采用AMMI模型对参试株系的树高性状进行稳定性评价,在帽儿山实验林场和生态实验林场生长稳定性较好的株系是:WT、FC11、FC16、FC19、FC28、FC29、FC30、FC31、C4;在石道河林场生长稳定性好的株系是:FC2、FC7、FC13、FC14、FC18、FC27、FC33、FC40、FC41、C6、C10、C17、C19。根据第一主成分分量IPC1及相对稳定性参数Dg值,对22个参试株系进行稳定性评价,结果显示:FC14、FC29、C19、FC27、FC41、WT、FC13、C4、C10、C6、FC16、FC7、FC28、FC40、C17、FC31、FC18属于高产稳产型株系;FC2、FC11、FC19、FC33属于高产非稳产型株系;FC30属于低产稳产型株系。  结论  转BpCCR1正义链及转反义链均能促进白桦树高生长,根据生长性状,选出17个株系作为在帽儿山林场、石道河林场及生态实验林场生长适应性好的高产株系。

     

  • 图  1  树高性状双标图

    Figure  1.  Biplot of tree height traits

    表  1  造林试验地点地理气候因子

    Table  1.   Geographical climate factors of afforestation test locations

    序号
    No.
    试验地点
    Test location
    纬度
    Latitude
    经度
    Longitude
    海拔
    Elevation/m
    年平均温度
    Annual average temperature/℃
    年降水量
    Annual precipitation/mm
    土壤类型
    Soil type
    无霜期
    Frost-free period/d
    1 帽儿山实验林场
    Maoershan Experimental Forest Farm
    45°23′N 127°30′E 477.8 2.4 700.0 暗棕壤
    Dark brown soil
    120
    2 石道河林场
    Shidaohe Forest Farm
    42°38′N 126°39′E 514.8 5.5 737.4 暗棕壤
    Dark brown soil
    135
    3 生态实验林场
    Ecological Experimental Forest Farm
    41°16′N 119°55′E 279.0 6.5 500.0 暗棕壤
    Dark brown soil
    128
    下载: 导出CSV

    表  2  3个试验地点的各试验株系数量

    Table  2.   Number of tested lines at each test site

    株系
    Line
    帽儿山实验林场
    Maoershan
    Experimental
    Forest Farm
    石道河林场
    Shidaohe
    Forest Farm
    生态实验林场
    Ecological
    Experimental
    Forest Farm
    WT 21 16 13
    C4 5 7 3
    C6 3 3 3
    C10 9 8 9
    C17 5 3 3
    C19 4 6 3
    FC2 8 6 3
    FC7 3 5 3
    FC11 3 3 3
    FC13 8 7 5
    FC14 7 4 3
    FC16 6 3 3
    FC18 5 3 3
    FC19 3 3 3
    FC27 3 3 3
    FC28 5 3 3
    FC29 3 3 3
    FC30 3 3 3
    FC31 4 3 3
    FC33 5 6 4
    FC40 6 4 3
    FC41 5 5 4
    总数 Total 124 107 86
    注:WT为野生型,C4、C6、C10、C17、C19为转正义链株系,FC2、FC7、FC11、FC13、FC14、FC16、FC18、FC19、FC27、FC28、FC29、FC30、FC31、FC33、FC40、FC41为转反义链株系。Notes: WT, wild-type; C4, C6, C10, C17; C19, trans sense strand lines; FC2, FC7, FC11, FC13, FC14, FC16, FC18, FC19, FC27, FC28, FC29, FC30, FC31, FC33, FC40, FC41, trans antisense strand lines.
    下载: 导出CSV

    表  3  各试验点参试株系生长性状方差分析

    Table  3.   ANOVA of growth traits of test lines at each test location

    性状
    Trait
    变异来源
    Source of variation
    dfSSMSFP
    树高
    Tree height
    总计
    Total
    316 241.929
    株系
    Line
    21 23.758 1.131 1.820* 0.017
    地点
    Location
    2 9.839 4.920 7.913** < 0.001
    株系 × 地点
    Line × location
    42 43.960 1.047 1.683** 0.008
    误差
    Error
    251 156.057 0.622
    胸径
    DBH
    总计
    Total
    316 380.457
    株系
    Line
    21 49.677 2.366 2.227** 0.002
    地点
    Location
    2 7.350 3.675 3.459* 0.033
    株系 × 地点
    Line × location
    42 50.215 1.196 1.125 0.287
    误差
    Error
    251 266.641 1.062
    材积
    Volume of wood
    总计
    Total
    316 0.001
    株系
    Line
    21 < 0.001 < 0.001 2.128** 0.003
    地点
    Location
    2 < 0.001 < 0.001 2.705 0.069
    株系 × 地点
    Line × location
    42 < 0.001 < 0.001 1.186 0.214
    误差
    Error
    251 0.001 < 0.001
    注:**表示在0.01水平上差异显著,*表示在0.05水平上差异显著。下同。Notes: ** indicates a significant difference at the 0.01 level, * indicates a significant difference at the 0.05 level. The same below.
    下载: 导出CSV

    表  4  不同试验地点转基因白桦生长性状的主要遗传参数

    Table  4.   Main genetic parameters of growth traits of transgenic birch in different test locations

    性状
    Trait
    试验地点
    Test site
    均值
    Mean
    标准差
    SD
    变幅
    Change range
    变异系数
    Coefficient of variation/%
    FP
    树高
    Tree height/m
    帽儿山林场
    Maoershan Experimental Forest Farm
    4.47 b 0.59 2.10 ~ 6.00 19.72 4.207** < 0.001
    石道河林场
    Shidaohe Forest Farm
    4.86 a 0.90 1.50 ~ 6.50 21.90 1.979** 0.006
    生态实验林场
    Ecological Experimental Forest Farm
    4.38 b 0.77 2.80 ~ 5.90 17.72 1.988* 0.014
    胸径
    DBH/cm
    帽儿山林场
    Maoershan Experimental Forest Farm
    3.87 0.86 1.10 ~ 5.90 30.42 4.012** < 0.001
    石道河林场
    Shidaohe Forest Farm
    3.85 1.03 0.80 ~ 6.30 32.04 2.457** 0.001
    生态实验林场
    Ecological Experimental Forest Farm
    4.06 1.05 1.70 ~ 6.00 26.62 1.267 0.222
    材积
    Volume of wood/m3
    帽儿山林场
    Maoershan Experimental Forest Farm
    0.003 1 0.001 5 0.000 8 ~ 0.008 4 62.48 3.718** < 0.001
    石道河林场
    Shidaohe Forest Farm
    0.003 5 0.001 9 0.000 6 ~ 0.010 0 63.81 2.975** < 0.001
    生态实验林场
    Ecological Experimental Forest Farm
    0.003 5 0.002 0 0.000 4 ~ 0.008 2 58.81 1.701* 0.046
    注:不同处理变量差异采用Duncans multiple-range差异性检验进行分析,不同的小写字母表示处理间差异达到显著水平(P < 0.05)。下同。Notes: Duncan multiple range difference test is used to analyze the differences of different treatment variables. Different small letters indicate that the differences between treatments have reached a significant level (P < 0.05). The same below.
    下载: 导出CSV

    表  5  帽儿山实验林场转基因白桦生长性状及保存率比较

    Table  5.   Comparison of growth characteristics and preservation rate of transgenic birch in Maoershan Experimental Forest Farm

    株系
    Line
    树高
    Tree height/m
    DBH/cm材积
    Volume of wood/m3
    保存率
    Preserving rate/%
    WT 3.91 ± 0.71de 3.11 ± 0.82 def 0.001 9 ± 0.001 2de 87.50
    C4 4.90 ± 0.10abc 4.40 ± 0.60abcde 0.004 0 ± 0.000 9abcde 71.43
    C6 3.73 ± 0.49e 3.13 ± 1.11 def 0.001 8 ± 0.001 4de 75.00
    C10 3.80 ± 0.46de 3.20 ± 0.44 cdef 0.001 8 ± 0.000 6e 81.82
    C17 4.43 ± 0.32abcde 3.13 ± 0.50 def 0.001 9 ± 0.000 4de 83.33
    C19 4.13 ± 0.32bcde 3.43 ± 0.32 bcdef 0.002 2 ± 0.000 6cde 80.00
    FC2 4.92 ± 0.24abc 4.43 ± 0.55abcd 0.004 1 ± 0.001 0abcde 80.00
    FC7 4.30 ± 0.79abcde 3.83 ± 0.59 abcdef 0.002 8 ± 0.001 4bcde 75.00
    FC11 4.28 ± 0.67abcde 4.07 ± 0.42 abcdef 0.003 1 ± 0.000 9abcde 100.00
    FC13 4.43 ± 0.25abcde 4.10 ± 0.17 abcdef 0.003 2 ± 0.000 4abcde 100.00
    FC14 4.47 ± 0.06abcde 3.90 ± 0.26 abcdef 0.002 9 ± 0.000 4bcde 58.33
    FC16 4.42 ± 0.58abcde 3.83 ± 0.85 abcdef 0.003 0 ± 0.001 6bcde 75.00
    FC18 5.13 ± 0.40a 4.97 ± 0.76a 0.005 4 ± 0.001 9a 100.00
    FC19 4.45 ± 0.35abcde 3.20 ± 1.00 cdef 0.002 3 ± 0.001 3cde 40.00
    FC27 4.33 ± 0.31abcde 3.00 ± 0.50 f 0.001 8 ± 0.000 7de 100.00
    FC28 4.93 ± 0.12abc 4.53 ± 0.29abc 0.004 3 ± 0.000 6abc 100.00
    FC29 4.70 ± 0.40abcde 4.87 ± 0.60a 0.004 7 ± 0.001 2ab 100.00
    FC30 3.95 ± 1.08cde 3.03 ± 1.36 ef 0.002 0 ± 0.001 5cde 100.00
    FC31 5.03 ± 0.45ab 4.40 ± 0.36abcde 0.004 1 ± 0.000 9abcd 100.00
    FC33 4.10 ± 0.30bcde 3.43 ± 0.67 bcdef 0.002 1 ± 0.000 6cde 83.33
    FC40 5.23 ± 0.61a 4.70 ± 1.06ab 0.005 1 ± 0.002 4ab 100.00
    FC41 4.73 ± 0.65abcd 4.37 ± 0.57 abcdef 0.003 9 ± 0.001 5abcde 100.00
    平均 Mean 4.47 ± 0.59 3.87 ± 0.86 0.003 1 ± 0.001 5 83.67
    下载: 导出CSV

    表  6  石道河林场转基因白桦生长性状及保存率比较

    Table  6.   Comparison of growth characteristics and preservation rate of transgenic birch in Shidaohe Forest Farm

    株系
    Line
    树高
    Tree height/m
    DBH/cm材积
    Volume of wood/m3
    保存率
    Preserving rate/%
    WT 4.86 ± 1.14abcd 4.01 ± 1.47abcde 0.004 0 ± 0.003 1abcd 76.19
    C4 4.50 ± 0.36abcde 4.03 ± 0.25abcde 0.003 1 ± 0.000 1bcde 100.00
    C6 5.00 ± 0.20abcd 4.20 ± 0.00abcd 0.003 7 ± 0.000 2abcde 66.67
    C10 5.05 ± 0.40abcd 3.67 ± 0.59 abcdef 0.003 0 ± 0.001 0bcde 80.00
    C17 5.70 ± 0.00a 4.25 ± 0.05abcd 0.004 4 ± 0.000 1abcd 50.00
    C19 4.87 ± 0.42abcd 3.43 ± 0.57 abcdef 0.002 6 ± 0.000 9bcde 100.00
    FC2 5.17 ± 0.25abcd 4.00 ± 0.98abcde 0.003 7 ± 0.001 7abcde 75.00
    FC7 5.57 ± 0.70a 5.07 ± 1.31a 0.006 4 ± 0.003 6a 83.33
    FC11 3.85 ± 0.15de 2.55 ± 0.05 ef 0.001 1 ± 0.000 0e 100.00
    FC13 5.53 ± 0.35ab 4.57 ± 0.23abc 0.004 9 ± 0.000 6abc 100.00
    FC14 5.03 ± 0.12abcd 3.80 ± 0.10 abcdef 0.003 1 ± 0.000 2bcde 40.00
    FC16 3.90 ± 1.18cde 3.43 ± 0.84 abcdef 0.001 9 ± 0.000 3de 60.00
    FC18 5.17 ± 0.25abcd 4.97 ± 0.15a 0.005 3 ± 0.000 1ab 75.00
    FC19 4.03 ± 1.82bcde 2.70 ± 1.85 def 0.002 1 ± 0.002 3cde 60.00
    FC27 4.70 ± 0.10abcde 2.95 ± 0.25 cdef 0.001 8 ± 0.000 3de 100.00
    FC28 4.65 ± 0.65abcde 3.50 ± 0.10 abcdef 0.002 5 ± 0.000 5bcde 100.00
    FC29 5.20 ± 0.80abcd 4.00 ± 0.80abcde 0.003 9 ± 0.001 9abcde 66.67
    FC30 3.27 ± 1.98e 2.30 ± 1.91 f 0.001 6 ± 0.002 0de 100.00
    FC31 4.40 ± 0.00abcde 3.30 ± 0.10 bcdef 0.002 1 ± 0.000 1cde 100.00
    FC33 5.80 ± 0.26a 5.03 ± 0.21a 0.006 1 ± 0.000 5a 100.00
    FC40 5.40 ± 0.53abc 4.20 ± 0.70abcd 0.004 2 ± 0.001 7abcd 100.00
    FC41 5.30 ± 0.26abcd 4.83 ± 0.42ab 0.005 2 ± 0.001 0ab 100.00
    平均 Mean 4.86 ± 0.90 3.85 ± 1.03 0.003 5 ± 0.001 9 80.00
    下载: 导出CSV

    表  7  生态实验林场转基因白桦生长性状及保存率比较

    Table  7.   Comparison of growth characteristics and preservation rate of transgenic birch in Ecological Experimental Forest Farm

    株系
    Line
    树高
    Tree height/m
    DBH/cm材积
    Volume of wood/m3
    保存率
    Preserving rate/%
    WT 4.45 ± 0.44abcde 4.19 ± 0.69 0.003 4 ± 0.001 3ab 61.90
    C4 4.60 ± 0.70abcde 4.15 ± 1.35 0.004 0 ± 0.002 6ab 28.57
    C6 3.55 ± 0.55e 3.60 ± 1.10 0.002 3 ± 0.001 5ab 50.00
    C10 4.02 ± 0.63bcde 3.73 ± 0.90 0.002 7 ± 0.001 5ab 69.23
    C17 3.95 ± 0.95cde 3.95 ± 0.95 0.003 0 ± 0.001 8ab 33.33
    C19 4.40 ± 0.10abcde 4.95 ± 0.05 0.004 5 ± 0.000 2ab 25.00
    FC2 3.50 ± 0.10e 3.20 ± 0.40 0.001 6 ± 0.000 4ab 25.00
    FC7 5.00 ± 0.60abcd 4.00 ± 1.00 0.003 8 ± 0.002 0ab 50.00
    FC11 4.93 ± 0.86abcd 4.57 ± 1.97 0.005 1 ± 0.003 6ab 75.00
    FC13 4.35 ± 0.73abcde 3.77 ± 0.81 0.002 8 ± 0.001 6ab 62.50
    FC14 4.27 ± 1.08abcde 3.80 ± 1.87 0.003 4 ± 0.003 8ab 21.43
    FC16 4.35 ± 0.75abcde 4.10 ± 1.30 0.003 7 ± 0.002 4ab 33.33
    FC18 3.80 ± 0.90de 3.55 ± 1.85 0.003 0 ± 0.002 6ab 40.00
    FC19 5.17 ± 0.64abc 4.20 ± 0.70 0.004 0 ± 0.001 7 b 33.33
    FC27 3.85 ± 0.05cde 4.00 ± 0.40 0.002 6 ± 0.000 5ab 66.67
    FC28 5.30 ± 0.60ab 5.10 ± 0.70 0.005 9 ± 0.002 1a 50.00
    FC29 5.00 ± 0.10abcd 5.45 ± 0.05 0.006 1 ± 0.000 0a 66.67
    FC30 4.10 ± 0.30abcde 3.15 ± 0.65 0.001 9 ± 0.000 8 b 66.67
    FC31 4.00 ± 0.60abcde 3.50 ± 1.20 0.002 5 ± 0.001 7ab 50.00
    FC33 5.37 ± 0.35a 4.52 ± 0.37 0.004 6 ± 0.000 8ab 57.14
    FC40 4.10 ± 1.35abcde 3.60 ± 1.45 0.002 9 ± 0.002 7ab 50.00
    FC41 4.28 ± 0.58abcde 4.25 ± 0.68 0.003 4 ± 0.001 4ab 80.00
    平均 Mean 4.38 ± 0.77 4.06 ± 1.05 0.003 5 ± 0.002 0 48.03
    下载: 导出CSV

    表  8  各试验点参试株系树高AMMI模型分析

    Table  8.   AMMI model of tree height of test line at each test location

    变异来源
    Source of variation
    dfSS方差分量 Variance component/%MSFP
    总计
    Total
    197 122.842 9 0.623 6
    处理
    Treatment
    65 66.455 7 1.022 4 2.393** < 0.001
    株系
    Line
    21 19.803 9 16.12 0.943 0 2.208** 0.004
    地点
    Location
    2 8.676 6 7.06 4.338 3 10.156** < 0.001
    株系 × 地点
    Line × location
    42 37.975 1 30.91 0.904 2 2.117** 0.001
    PCA1 22 24.168 7 63.64 1.098 6 2.572** 0.001
    PCA2 20 13.806 5 36.36 0.690 3 1.616 0.058
    残差
    Residual error
    0 0.000 0 0.00 0.000 0
    误差
    Error
    132 56.387 2 0.427 2
    下载: 导出CSV

    表  9  各试验点参试株系交互作用主成分轴分量值及稳定性参数

    Table  9.   Main component axial component values and stability parameters of interactions of test lines at each test location

    项目
    Item
    变量
    Variable
    树高平均值
    Average tree height/m
    离差
    Deviation
    IPC1IPC2Dg
    株系
    Line
    FC14 4.59 0.019 0.120 0.008 0.120
    FC29 4.97 0.397 −0.109 0.148 0.184
    C19 4.47 −0.103 0.006 0.194 0.195
    FC27 4.29 −0.275 0.146 −0.130 0.196
    FC41 4.77 0.203 0.220 −0.071 0.231
    WT 4.41 −0.164 −0.009 0.335 0.335
    FC13 4.77 0.203 0.306 0.173 0.352
    C4 4.67 0.097 −0.262 −0.258 0.367
    C10 4.29 −0.278 0.250 0.307 0.396
    C6 4.09 −0.475 0.419 0.182 0.457
    FC16 4.22 −0.348 −0.406 −0.213 0.458
    FC7 4.96 0.386 0.065 0.460 0.464
    FC28 4.96 0.391 −0.478 −0.020 0.478
    FC40 4.91 0.341 0.319 −0.382 0.498
    C17 4.69 0.125 0.539 0.088 0.546
    FC31 4.48 −0.092 −0.070 −0.544 0.549
    FC18 4.70 0.130 0.341 −0.477 0.587
    FC30 3.77 −0.798 −0.566 −0.185 0.595
    FC2 4.53 −0.042 0.469 −0.451 0.651
    FC11 4.35 −0.216 −0.657 0.036 0.658
    FC19 4.55 −0.020 −0.676 0.057 0.679
    FC33 5.09 0.519 0.028 0.742 0.743
    地点
    Location
    帽儿山实验林场
    Maoershan Experimental Forest Farm
    4.47 −0.101 −0.102 1.193 1.197
    石道河林场
    Shidaohe Forest Farm
    4.86 −0.191 −1.137 −0.673 1.321
    生态实验林场
    Ecological Experimental Forest Farm
    4.38 0.292 1.239 −0.519 1.344
    注:Dg为相对稳定性参数。Note: Dg is a relative stability parameter.
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-08-29
  • 录用日期:  2022-06-29
  • 修回日期:  2021-01-20
  • 网络出版日期:  2022-07-01
  • 刊出日期:  2022-08-02

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