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    山东大青山刺槐无性系叶性状的表型变异分析与饲用优良无性系选择

    Phenotypic variation analysis on leaf traits and selection of optimal forage clones of Robinia pseudoacacia clones in Shandong Province of eastern China

    • 摘要:
        目的  为系统了解山东大青山林场刺槐的无性系变异规律,并初步筛选饲用型刺槐优良无性系。
        方法  对332个刺槐无性系的13个叶表型性状进行多样性、隶属函数、相关性和聚类分析,并在聚类分析的基础上,针对不同性状进行各组间差异分析。
        结果  13个性状的总变异系数为14.883%,其中,4个复叶和9个小叶性状的变异系数分别为16.062%和14.360%。该刺槐资源的隶属函数值介于0.196 ~ 0.717之间,其中无性系SD328拥有最大值(0.717),其在复叶宽、小叶面积和小叶柄角3个性状上具有明显优势。表型和遗传相关性表明,小叶对数与小叶数量有最大的正相关性系数,小叶长/宽与小叶圆度的负相关性系数最大。主成分分析表明,4个主成分因子可以代表刺槐无性系86.447%的原始数据信息。当平方欧式距离为8时进行聚类分析,332份刺槐无性系被分为5类。
        结论  通过系统分析刺槐无性系种质的表型多样性,初步筛选出饲用性刺槐无性系72份,为山东大青山饲用刺槐的选育和种质保存等研究奠定基础。

       

      Abstract:
        Objective  The objective of this experiment was to systematically understand the phenotypic variation rule of Robinia pseudoacacia clones in Feixian County State Owned Daqingshan Forest Farm of Shandong Province, eastern China and selection of optimal forage varieties.
        Method  Diversity, subordinate function, correlation and cluster analysis were performed on 13 leaf phenotypic traits of 332 R. pseudoacacia clones, and on the basis of cluster analysis, the differences among groups were analyzed for different traits.
        Result  The total coefficient of variation of the 13 traits was 14.883%, of which the coefficients of variation of the 4 compound leaves and 9 leaflets were 16.062% and 14.360%, respectively. The subordinate function value of this R. pseudoacacia was between 0.196 and 0.717, in which SD328 clone had the maximum value (0.717) and obvious advantages in the three characteristics of compound leaf width, leaflet area and petiole angle. The phenotype and genetic correlation showed that the leaflet pairs had the largest positive correlation coefficient with the leaflet numbers, and the leaflet length/width had the largest negative correlation coefficient with the leaflet circularity. Principal component analysis showed that the four principal component factors could represent 86.447% of the original data information of R. pseudoacacia clones. When the squared euclidean distance was 8, cluster analysis was performed, and 332 clones of R. pseudoacacia were divided into 5 categories.
        Conclusion  The phenotypic diversity of the R. pseudoacacia germplasm resources was systematically analyzed, seventy-two accessions were selected based on the breeding of ornamental and forage tree species, in order to lay the foundation for the research of breeding and germplasm preservation of R. pseudoacacia.

       

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