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乙烯对春石斛类原球茎玻璃化超低温保存存活率的影响

张玲玲 任瑞芬 姜雪茹 周好 刘燕

张玲玲, 任瑞芬, 姜雪茹, 周好, 刘燕. 乙烯对春石斛类原球茎玻璃化超低温保存存活率的影响[J]. 北京林业大学学报, 2021, 43(6): 101-107. doi: 10.12171/j.1000-1522.20200308
引用本文: 张玲玲, 任瑞芬, 姜雪茹, 周好, 刘燕. 乙烯对春石斛类原球茎玻璃化超低温保存存活率的影响[J]. 北京林业大学学报, 2021, 43(6): 101-107. doi: 10.12171/j.1000-1522.20200308
Zhang Lingling, Ren Ruifen, Jiang Xueru, Zhou Hao, Liu Yan. Effects of ethylene on the viability of cryopreserved Dendrobium protocorm-like bodies[J]. Journal of Beijing Forestry University, 2021, 43(6): 101-107. doi: 10.12171/j.1000-1522.20200308
Citation: Zhang Lingling, Ren Ruifen, Jiang Xueru, Zhou Hao, Liu Yan. Effects of ethylene on the viability of cryopreserved Dendrobium protocorm-like bodies[J]. Journal of Beijing Forestry University, 2021, 43(6): 101-107. doi: 10.12171/j.1000-1522.20200308

乙烯对春石斛类原球茎玻璃化超低温保存存活率的影响

doi: 10.12171/j.1000-1522.20200308
基金项目: 国家自然科学基金项目(31770741)
详细信息
    作者简介:

    张玲玲。主要研究方向:花卉种质创新与育种。Email:1637946084@qq.com 地址:100083 北京市海淀区清华东路35号北京林业大学园林学院

    责任作者:

    刘燕,教授,博士生导师。主要研究方向:园林植物应用与园林生态、花卉种植资源与遗传育种、花卉繁殖与栽培。Email:chblyan@163.com 地址:同上

  • 中图分类号: S682.31

Effects of ethylene on the viability of cryopreserved Dendrobium protocorm-like bodies

  • 摘要:   目的  为探究乙烯对PLBs超低温保存存活率的影响,以期为提高其存活率开拓思路。  方法  以春石斛‘红梦幻’类原球茎(PLBs)为材料,分别在其玻璃化超低温保存的预培养和装载阶段添加外源乙烯供体乙烯利和乙烯合成抑制剂硝酸钴,测定PLBs超低温保存过程中内源乙烯前体1-氨基环丙烷羧酸(ACC)含量、ACC氧化酶(ACO)活性及PLBs液氮保存后细胞存活率。  结果  结果显示:无论在预培养还是装载阶段,外源乙烯供体乙烯利处理后,超低温保存过程中PLBs内源ACC含量和ACO活性均显著提高,同时液氮保存后存活率也显著提高;在这两个阶段添加外源乙烯合成抑制剂硝酸钴后,超低温保存过程中PLBs内源ACC含量、ACO活性显著降低,液氮保存后存活率也显著降低。相关分析显示,PLBs液氮冻后存活率与其内源ACC含量和ACO活性呈极显著正相关。  结论  本研究表明,外源乙烯调节剂乙烯利和硝酸钴通过调节内源乙烯含量而对玻璃化超低温保存的春石斛类原球茎存活率产生显著影响。

     

  • 图  1  预培养阶段添加外源乙烯调节剂乙烯利和硝酸钴对超低温保存后春石斛‘红梦幻’PLBs存活率的影响

    图中数据用3个重复的平均值 ± 标准误表示,同阶段下不同小写字母表示各处理间差异显著(P < 0.05)。下同。Each bar represents mean ± SE over four repetitions. Columns with different letters are significantly different (P < 0.05). The same below.

    Figure  1.  Effects of adding exogenous ethylene regulator ETH and Co at the preculture stage on the survival rate of Dendrobiumnobile ‘Hamana Lake Dream’ PLBs after cryopreservation

    图  2  装载阶段添加外源乙烯调节剂乙烯利和硝酸钴对超低温保存后春石斛‘红梦幻’PLBs存活率的影响

    Figure  2.  Effects of adding exogenous ethylene regulator ETH and Co at the loading stage on the survival rate of D. nobile ‘Hamana Lake Dream’ PLBs after cryopreservation

    图  3  外源乙烯调节剂乙烯利和硝酸钴的添加对玻璃化超低温保存中PLBs中内源ACC含量的影响

    A、B分别表示在预培养阶段和装载阶段添加外源乙烯调节剂(乙烯利和硝酸钴)对玻璃化超低温保存下PLBs内源ACC含量的影响。Pr. 预培养阶段;L. 装载阶段;UNL. 去装载阶段;CK. 常规玻璃化程序;Pr+Eth和Pr+Co分别指预培养阶段添加乙烯利和硝酸钴的玻璃化程序;L+Eth和L+Co分别指装载阶段添加乙烯利和硝酸钴的玻璃化程序。下同。A, B are the effects of exogenous ethylene added in preculture medium or loading stage on endogenous ACC in PLBs under cryopreservation. Pr, preculture stage; L, loading stage; UNL, unloading stage; CK, common vitrification procedure; Pr+ETH and Pr+Co refer to vitrification procedure with ETH or Co addition at the preculture stage, respectively; L+ETH and L+Co refer to vitrification procedure with ETH or Co addition at the loading stage, respectively. The same below.

    Figure  3.  Effects of the addition of exogenous ethylene regulators ETH and Co on the content of endogenous ACC in PLBs during vitrification cryopreservation

    图  4  外源乙烯调节剂乙烯利和硝酸钴的添加对玻璃化超低温保存中PLBs中内源ACO活性的影响

    Figure  4.  Effects of the addition of exogenous ethylene regulators ETH and Co on the activity of endogenous ACO in PLBs during vitrification cryopreservation

    表  1  存活率和内源ACC、ACO的简单相关系数矩阵

    Table  1.   Correlation coefficient matrix analysis of survival rate, endogenous ACC content and ACO activity

    指标 Index 存活率 Survival rate ACCACO
    存活率 Survival rate1.0000.741*0.956**
    ACC1.0000.732*
    ACO1.000
    注:**表示差异极显著(P < 0.01),*表示差异显著(P < 0.05)。
    Notes: ** indicates that the difference is extremely significant (P < 0.01), and * indicates that the difference is significant (P < 0.05).
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-10-14
  • 修回日期:  2020-11-02
  • 网络出版日期:  2021-06-03
  • 刊出日期:  2021-06-30

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