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    姜虹, 孟昭军, 李鹏, 薛羿, 姜礅, 李国江, 严善春. 带状混交对长白落叶松防御蛋白活性的影响[J]. 北京林业大学学报, 2022, 44(11): 82-89. DOI: 10.12171/j.1000-1522.20210249
    引用本文: 姜虹, 孟昭军, 李鹏, 薛羿, 姜礅, 李国江, 严善春. 带状混交对长白落叶松防御蛋白活性的影响[J]. 北京林业大学学报, 2022, 44(11): 82-89. DOI: 10.12171/j.1000-1522.20210249
    Jiang Hong, Meng Zhaojun, Li Peng, Xue Yi, Jiang Dun, Li Guojiang, Yan Shanchun. Effects of banding mixed forest on activity of defensive proteins in Larix olgensis[J]. Journal of Beijing Forestry University, 2022, 44(11): 82-89. DOI: 10.12171/j.1000-1522.20210249
    Citation: Jiang Hong, Meng Zhaojun, Li Peng, Xue Yi, Jiang Dun, Li Guojiang, Yan Shanchun. Effects of banding mixed forest on activity of defensive proteins in Larix olgensis[J]. Journal of Beijing Forestry University, 2022, 44(11): 82-89. DOI: 10.12171/j.1000-1522.20210249

    带状混交对长白落叶松防御蛋白活性的影响

    Effects of banding mixed forest on activity of defensive proteins in Larix olgensis

    • 摘要:
        目的  为探明混交林水曲柳化感作用对落叶松防御蛋白的影响,本研究分析了与水曲柳带状混交模式下长白落叶松针叶内防御蛋白活性的变化。
        方法  以长白落叶松为对照,将盆栽和地栽方式种植的长白落叶松与水曲柳分别按1∶1、3∶3、5∶5(HJ1∶1、HJ3∶3、HJ5∶5)带状混交,1年后利用紫外分光光度计测定各处理长白落叶松针叶内防御蛋白活性。
        结果  地栽、盆栽3种带状混交长白落叶松针叶内防御蛋白活性多高于对照。地栽HJ3∶3针叶内苯丙氨酸解氨酶(PAL)、胰凝乳蛋白酶抑制剂(CI)、胰蛋白酶抑制剂(TI)活性及HJ5∶5针叶内多酚氧化酶(PPO)活性始终高于对照(P < 0.05);除8月1日外,地栽HJ3∶3 针叶内PPO活性显著高于对照(P < 0.05),HJ3∶3 与HJ5∶5差异均不显著(P > 0.05)。盆栽HJ3∶3针叶内防御蛋白活性始终高于对照(P < 0.05),且HJ3∶3多显著高于HJ1∶1或HJ5∶5。与盆栽相比,地栽HJ5∶5更有利于提高针叶内PAL活性;而地栽HJ1∶1对针叶内PPO活性及HJ5∶5对针叶内CI活性影响较小。
        结论  与水曲柳带状混交,能够提高长白落叶松针叶内防御蛋白活力,以HJ3∶3混交模式效果最为显著,其防御蛋白活力明显增强。

       

      Abstract:
        Objective  To illuminate the effects of allelogenicity of Fraxinus mandshurica on defense proteins of larch in mixed forest, the activities of defensive proteins in larch conifers of banding mixed forest types with Fraxinus mandshurica were analyzed.
        Method  Taking Larix olgensis as the control, that planted in pots and in the ground was mixed with Manchurian ash in 1∶1, 3∶3 and 5∶5 (HJ1∶1, HJ3∶3, HJ5∶5) bands, respectively. One year later, the activity of defense proteins in the needles of Larix olgensis was measured by ultraviolet spectrophotometer.
        Result  The activities of defense proteins in needles of three kinds of banded mixed Larix olgensis planted in the field and pot were higher than those in the control. The activities of phenylalanine ammonia lyase (PAL), chymotrypsin inhibitor (CI), trypsin inhibitor (TI) and polyphenol oxidase (PPO) in HJ3∶3 needles were always higher than those in the control (P<0.05). Except August 1, the PPO activity in the needles of HJ3∶3 planted in the field was significantly higher than that in control (P<0.05), and there was no significant difference between HJ3∶3 and HJ5∶5 (P>0.05). The activity of defense protein in potted HJ3∶3 needles was always higher than that in the control (P<0.05), and HJ3∶3 was significantly higher than HJ1∶1 or HJ5∶5. Compared with pot culture, field planting HJ5∶5 was more beneficial to increase PAL activity in needles. However, the effects of HJ1∶1 on PPO activity in needles and HJ5∶5 on CI activity in needles were relatively small.
        Conclusion  The zonal mixing with Fraxinus mandshurica can improve the activity of defense protein in the needles of Larix olgensis, and the HJ3∶3 mixing mode has the most significant effect, and its defensive protein activity is significantly enhanced.

       

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