Abstract:
Objective The function of actin depolymerization factor (ADF) family genes in Malus micromalus in response to pathogen stress was identified, which provided a certain reference for the genetic improvement of disease resistance in Malus micromalus.
Method Firstly, four candidate genes MmADF1, MmADF3, MmADF8 and MmADF11 in response to pathogen stress were screened. The expression patterns and cis-acting elements of these four genes were analyzed to construct MmADF1 and MmADF11 overexpression vectors. These vectors were introduced into the leaves of Malus micromalus and its related plant Gala (Malus pumila) by vacuum infiltration transformation, and then inoculated with pathogenic Alternaria R1 on the leaves and petals. By counting the infection efficiency, infection area and the percentage of cell death, the role of these genes in resisting pathogen infection was clarified.
Result The results showed that the expression levels of MmADF1 and MmADF11 in infected plant tissues were significantly higher than those of other genes. Overexpression of MmADF1 and MmADF11 can significantly reduce the stomatal aperture of Malus micromalus leaves and enhance the ability of Malus micromalus to resist pathogens. In addition, MmADF1 and MmADF11 showed certain disease resistance in different tissues and organs, which indicated that ADF was conservative in different plant tissues.
Conclusion This study illustrates the important role of MmADF1 and MmADF11 in the resistance of Malus micromalus to fungi by different plants, and provides a theoretical basis for regulation of ADF gene and cultivation of disease-resistant Malus micromalus by molecular technology.
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