Abstract: The composition of polyphenols from Pinus sylvestris L. var. mongolica were analyzed and their structures were identified with UV-VIS, IR, HPLC and LC-MS. The UV-VIS results showed that there are absorption peaks in regions of 240-- 280 nm and 300-- 400 nm, indicating that flavonoids exist in the pine polyphenols. Fourier Transform Infrared Spectrum analysis showed that a strong and broad absorption peak at 3 400 cm-1 is generated from stretching and vibration of phenolic hydroxyl Ar-OH and absorption peaks at 1 390-- 1 315 cm-1 due to characteristics of in-plane bending vibration of hydroxyl δO—H, which indicated that hydroxyl groups existed in the pine polyphenols. Combined application of HPLC and LC-MS showed that there are seven components in P. sylvestris L. var. mongolica polyphenols, i.e., catechuic acid, rutin, chlorogenic acid, gallic acid, sinapic acid-dextrose glucoside, peony-3- glucoside or centaurea-3-(6-o-acetyl)-glucose glycoside, and sinapic acid peonidin-3-sophoroside composition.